Choice of anticoiagulant can influence the analysis using fluorescence in situ hybridization of fetal cells enriched from maternal blood

Prenatal Diagnosis

Volumn 20, Issue 3, 2000, Pages 257-259

  Brombacher, Verena ^a   Kiefer, Vivian ^a   Troeger, Carolyn ^a   Vial, Yvan ^b   Minderer, Sabine ^c   Holzgreve, Wolfgang ^a   Hahn, Sinuhe ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

^b LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^c Frauenarzte und Medizinische Genetik   (Germany)

Author keywords

Fetal cells; Maternal blood; Prenatal diagnosis

Indexed keywords

ANTICOAGULANT AGENT; EDETIC ACID; HEPARIN;

ARTICLE; CELL SELECTION; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FETUS CELL; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN CELL; MATERNAL BLOOD; PRENATAL DIAGNOSIS; PRIORITY JOURNAL; SEX DIAGNOSIS; BLOOD CELL; BLOOD SAMPLING; CELL DEATH; CYTOLOGY; DRUG EFFECT; FEMALE; FETUS; IMMUNOMAGNETIC SEPARATION; MALE; METHODOLOGY; PREGNANCY; SENSITIVITY AND SPECIFICITY;

ANTICOAGULANTS; BLOOD CELLS; BLOOD SPECIMEN COLLECTION; CELL DEATH; EDETIC ACID; FEMALE; FETUS; HEPARIN; HUMANS; IMMUNOMAGNETIC SEPARATION; IN SITU HYBRIDIZATION, FLUORESCENCE; MALE; PREGNANCY; SENSITIVITY AND SPECIFICITY;

EID: 0033621935     PISSN: 01973851     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0223(200003)20:3<257::AID-PD781>3.0.CO;2-V     Document Type: Article

References (13)

1. Blanchi DW, Mahr A, Zickwolf GK, Houseal TW, Flint AF, Klinger KW. 1992. Detection of fetal cells with 47,XY, + 21 karyotype in maternal peripheral blood. Hum Genet 90: 368-370.
2. Cheung MC, Goldberg JD, Kann YW. 1996. Prenatal diagnosis of sickle cell anaemia and thalassaemia by analysis of fetal cells in maternal blood. Nat Genet 14: 264-268.
3. de la Cruz F, Shifrin H, Elias S, Bianchi DW, Jackson L, Evans MI, Simpson JL, Holzgreve W, Klinger K. 1998. Low false-positive rate of aneuploidy detection using fetal cells isolated from maternal blood. Fetal Diagn Ther 13: 380.
4. Elias S, Price J, Dockter M, Wachtel S, Tharapel A, Simpson JL, Klinger KW. 1992. First trimester prenatal diagnosis of trisomy 21 in fetal cells from maternal blood. Lancet 340: 1033.
5. Gänshirt-Ahlert D, Börejesson-Stoll R, Burschyk M, Dohr A, Gerritsen HSP, Helmer E, Miny P, Velasco M, Walde C, Patterson D, Teng N, Bhat NM, Bieber MM, Holzgreve W. 1993. Detection of fetal trisomies 21 and 18 from maternal blood using triple gradient and magnetic cell sorting. Am J Reprod Immun 30: 194-201.
6. Hahn S, Sant R, Holzgreve W. 1998. Fetal cells in maternal blood: current and future perspectives. Mol Hum Reprod 4: 515-521.
7. Hahn S, Kiefer V, Brombacher V, Troeger C, Holzgreve W. 1999. Fetal cells in maternal blood: an update from Basel. Eur J Obstet Gynecol Reprod Biol 85: 101-104.
8. Holzgreve W. 1997. Will ultrasound-screening and ultrasound-guided procedures be replaced by non-invasive techniques for the diagnosis of fetal chromosome anomalies? Editorial. Ultrasound Obstet Gynecol 9: 217-219.
9. Holzgreve W, Garritsen HS, Ganshirt Ahlert D. 1992. Fetal cells in the maternal circulation. J Reprod Med 37: 410-418.
10. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press: New York.
11. Sekizawa A, Kimura T, Sasaki M, Nakamura S, Kobayashi R, Sato T. 1996. Prenatal diagnosis of Duchenne muscular dystrophy using a single fetal nucleated erythrocyte in maternal blood. Neurology 46: 1350-1353.
12. Smits G, Holzgreve W, Hahn S. An examination of different Percoll density gradients in combination with MACS for the enrichment of fetal erythroblasts. Arch Gynecol Obstet (in press).
13. Troeger C, Holzgreve W, Hahn S. 1999. A comparison of different density gradients and antibodies for enrichment of fetal erythroblasts by MACS. Prenat Diagn 19: 521-526.