The effect of temperature and protein synthesis on the renaturation of firefly luciferase in intact H9c2 cells

Cellular and Molecular Life Sciences

Volumn 55, Issue 11, 1999, Pages 1473-1481

  Souren, J E M ^a   Wiegant, F A C ^a   Van Hof, P ^a   Van Aken, J M ^a   Van Wijk, R ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

H9c2 myoblasts; Heat shock; Heat shock protein; Luciferase; Luminescence

Indexed keywords

CYCLOHEXIMIDE; HEAT SHOCK PROTEIN; LUCIFERASE;

ANIMAL CELL; ARTICLE; ENZYME ACTIVITY; ENZYME DENATURATION; GENETIC TRANSFECTION; HEAT SHOCK; HYPERTHERMIA; NONHUMAN; PROTEIN SYNTHESIS; RAT; RENATURATION; TEMPERATURE;

ANIMALS; BEETLES; CELL LINE; CYCLOHEXIMIDE; HEAT; HEAT-SHOCK PROTEINS; LUCIFERASES; PROTEIN RENATURATION; PROTEIN SYNTHESIS INHIBITORS; RATS; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 0033618871     PISSN: 1420682X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s000180050386     Document Type: Article

References (32)

1. Nover L. (1991) Heat Shock Response, CRC, Boca Raton, FL
2. Parsell D. A. and Lindquist S. (1994) Heat shock proteins and stress tolerance. In: The Biology of Heat Shock Proteins and Molecular Chaperones, pp. 457-494, Morimoto R. L., Tissières A. and Georgopoulos C. (eds), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
3. Morimoto R. I., Jurivich D. A., Kroeger P. E., Mathur S. K., Murphy S. P., Nakai A. et al. (1994) Regulation of heat shock gene transcription by a familiy of heat shock factors. In: The Biology of Heat Shock Proteins and Molecular Chaperones, pp. 417-455, Morimoto R. I., Tissières A. and Georgopoulos C. (eds), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
4. Kampinga H. H. (1993) Thermotolerance in mammalian cells: protein denaturation and aggregation, and stress proteins. J. Cell. Sci, 104; 11-17
5. Parsell D. A. and Lindquist S. (1993) The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu. Rev. Genet. 27: 437-496
6. Bensaude O., Bellier S., Dubois M.-F., Giannoni F. and Nguyen V. T. (1996) Heat-shock induced protein modifications and modulation of enzyme activities, In: Stress-Inducible Cellular Responses, pp. 199-219, Feige U., Morimoto R. I., Yahara I. and Polla B. S. (eds), Basel, Birkhauser
7. Hahn G. M. and Li G. C. (1990) Thermotolerance, thermoresistance and thermosensitization. In: Stress Proteins in Biology and Medicine, pp. 79 100, Morimoto R. I., Tissieres A. and Georgopoulos C. (eds), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
8. Laszlo A. (1992) The thermoresistant state: protection from initial damage or better repair? Exp. Cell. Res. 202: 519-531
9. Li G. C., Mivechi N. F.and and Weitzel G. (1995) Heat shock proteins, thermotolerance, and their relevance to clinical hyperthermia. Int. J. Hyperthermia 11: 459-488
10. Lindegaard J. C. and Overgaard J. (1990) Step-down heating in a C3H mammary carcinoma in vivo: effects of varying the time and temperature of the sensitizing treatment. Int, J. Hyperthermia 6: 607-617
11. Wiegant F. A. C., Souren J. E. M., Van Rijn J. and Van Wijk R. (1993) Arsenite-induced sensitization and self-tolerance of Reuber H35 hepatoma cells. Cell. Biol. Toxicol. 9: 49-59
12. Wiegant F. A. C., Van Rijn J. and Van Wijk R. (1997) Enhancement of the stress response by minute amounts of cadmium in sensitized Reuber H35 hepatoma cells. Toxicology 116: 27-37
13. Vitn Wijk R., Ovelgönne J. H., Koning E. de, Jaarsveld K., Van Rijn J. and Wiegant F. A. C. (1994) Mild step-down heating causes increased transcription leveis of Hsp68 and Hsp84 mRNA and enhances thermotolerance development in Reuber H35 hepatoma cells. Int. J. Hyperthermia 10: 115-125
14. Ovelgönne J. H., Wiegant F. A. C., Souren J. E. M., Van Rijn J. and Van Wijk R. (1995) Enhancement of the stress response by low concentrations of arsenite in arsenite-pretreated H35 hepatoma cells. Toxicol. Appl. Pharmacol. 132: 146-155
15. Wiegant F. A. C., Spieker N. and Van Wijk R. (1998) Stressor-specific enhancement of Hsp induction by low doses of stressors in conditions of self-and cross-sensitization. Toxicology 127: 107-119
16. Wiegant F. A. C., Souren J. E. M. and Van Wijk R. (1999) Stimulation of survival capacity in heat shocked cells by subsequent exposure to minute amounts of chemical stressors: role of similarity in hsp-inducing effects. Hum. Exp. Toxicol, 18: 460-470
17. Morimoto R. I. (1993) Cells in stress: transcriptional activation of heat shock genes. Science 259: 1409-1410
18. Voellmy R. (1996) Sensing stress and responding to stress. In: Stress-Inducible Cellular Responses, pp. 121-137, Feige U., Morimoto R. I., Yahara I. and Polla B. S. (eds), Birkhäuser, Basel
19. Peper A., Grimbergen C. A., Spaan J. A. E., Souren J. E. M. and Van Wijk R. (1998) A mathematical model of the Hsp70 regulation in the cell. Int. J. Hyperthermia 14: 97-104
20. Ovelgönne J. H. and Van Wijk R. (1995) Modulation of Hsp68 gene expression after heat shock in thermosensitized and thermotolerant cells is not solely regulated by binding of HSF to HSE. Int. J. Hyperthermia 11: 719-732
21. Nguyen V. T., Morange M. and Bensaude O. (1989) Protein denaturation during heat shock and related stress: Escherichia coli β-galactosidase and Photinus pyralis luciferase inactivation in mouse cells, J. Biol. Chem, 264: 10487-10492
22. Pinto M., Morange M. and Bensaude O. (1991) Denaturation of proteins during heat shock: in vivo recovery of solubility and activity of reporter enzymes. J. Biol. Chem, 266: 13941-13946
23. Forreiter C., Kirschner M. and Novor L. (1997) Stable transformation of an Arabidopsis cell suspension culture with firefly luciferase providing a cellular system for analysis of chaperone activity in vivo. Plant Cell 9: 2171-2181
24. Souren J. E. M., Wiegant F. A. C. and Van Wijk R. (1999.) The role of hsp70 in protection and repair of luciferase activity in vivo; experimental data and mathematical modelling. Cell, Mol. Life Sci 55: 799-811
25. Laemmli U. K. (1970) Cleavage of the structural protein during the assembly of the head of the bacteriophage T4. Nature 227: 680-686
26. Souren J. E. M., Van Der Mast C. and Van Wijk R. (1999) NADPH-oxidase-dependent superoxide production by myocyte-derived H9c2 cells: influence of ischemia, heat shock, cycloheximide and eytochalasin D. J. Mol. Cell. Cardiol. 29: 2803-2812
27. Lindquist S. (1993) Autoregulation of the heat-shock response. In: Translational Regulation of Gene Expression 2, pp. 279-320, Han J. (ed.), Plenum, New York
28. Lee G. J., Roseman A. M., Saibil H. R. and Vierling E. (1997) A small heat shock protein stably binds heat-denatured model substrates and can maintain a substrate in a folding-competent state. EMBO J. 16: 659-671
29. Thulasiraman V. and Matts R. L. (1996) Effect of geldanamycin on the kinetics of chaperone-mediated renaturation of firefly luciferase in rabbit reticulocyte lysate, Biochemistry 35: 13443-13450
30. Schumacher R. J., Hansen W. J., Freeman B. C., Alnemri E., Litwack G. and Toft D. O. (1996) Cooperative action of Hsp70, Hsp90, and DnaJ proteins in protein renaturation. Biochemistry 35: 14889-14898
31. Schneider C., Sepplorenzino L., Nimmesgern E., Ouerfelli O., Danishetsky S., Rosen N. et al. (1996) Pharmacologic shifting of a balance between protein refolding and degradation mediated by Hsp90. Proc. Natl. Acad. Sci. USA 93: 14536-14541
32. Johnson B. D., Schumacher R. J., Ross, E. D. and Toft D. O. (1998) Hop modulates Hsp70/Hsp90 interactions of protein folding. J. Biol. Chem. 273: 3679-3686