The effect of trematode infection on amphibian limb development and survivorship

Science

Volumn 284, Issue 5415, 1999, Pages 802-804

  Johnson, Pieter T J ^a,c   Lunde, Kevin B ^a,c   Ritchie, Euan G ^b   Launer, Alan E ^a  

^a STANFORD UNIVERSITY   (United States)

^b JAMES COOK UNIVERSITY   (Australia)

^c CLAREMONT MCKENNA COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHIBIA; ARTICLE; CERCARIA; LIMB DEFORMITY; LIMB DEVELOPMENT; METAMORPHOSIS; NONHUMAN; PRIORITY JOURNAL; SURVIVAL RATE; TREMATODIASIS;

ANIMALS; ANURA; CALIFORNIA; HINDLIMB; METAMORPHOSIS, BIOLOGICAL; TREMATODA; TREMATODE INFECTIONS;

EID: 0033617521     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5415.802     Document Type: Article

References (26)

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13. Snails (P. tenuis) infected with Alaria or Ribeiroia were collected from California field sites, placed in 50-ml vials of commercial spring water, and allowed to shed cercariae for 8 to 10 hours. Within 1 hour after shedding, emerging cercariae were isolated, counted, and added to 100-ml specimen cups containing individual tadpoles. Tadpoles were exposed to cercariae for 90 min. No cercariae were observed in the specimen cups following the exposure period, suggesting that all had penetrated the tadpoles. The control group tadpoles were placed in specimen cups containing only spring water and returned to their containers after 90 min.
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26. We thank D. Zelmer, P. Basch, D. Barton, M. Poteet, and A. Smyth for assistance with parasite histology and taxonomy; J. Reaser for consultation on the design of field research protocols; N. Van Zwol for laboratory facilities; C. and S. Hoebich for support and encouragement; S. Ptak for statistical recommendations; R. Hershler and L. Kools for snail identifications; S. Khandwala for assistance with field work and resources; Santa Clara County Parks Division for property access; S. Holt, S. Sessions, and M. Scott for technical photography assistance; and P. Basch, A. Blaustein, C. Boggs, G. Daily, P. Ehrlich, E. Fleishman, C. Lunde, P. Vitousek, and C. Wheat for helpful comments on the manuscript.