Conversion of cucumber linoleate 13-lipoxygenase to a 9-lipoxygenating species by site-directed mutagenesis

Proceedings of the National Academy of Sciences of the United States of America

Volumn 96, Issue 7, 1999, Pages 4192-4197

  Hornung, Ellen ^a   Walther, Matthias ^b   Kühn, Hartmut ^b   Feussner, Ivo ^a  

^a LEIBNIZ INSTITUTE OF PLANT BIOCHEMISTRY   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Active site modeling; Cucumis sativus L.; Oxygenated lipids; Product specificity

Indexed keywords

FATTY ACID; LIPOXYGENASE; TRILINOLEIN;

AMINO ACID SUBSTITUTION; ARTICLE; CUCUMBER; ENZYME MODIFICATION; GENE ACTIVITY; GENE MUTATION; NONHUMAN; POSITION EFFECT; PRIORITY JOURNAL; PROTEIN EXPRESSION; REACTION TIME; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ARABIDOPSIS; BINDING SITES; COMPUTER SIMULATION; CUCUMIS SATIVUS; HISTIDINE; KINETICS; LIPOXYGENASE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SOLANUM TUBEROSUM; SUBSTRATE SPECIFICITY; VALINE;

CUCUMIS SATIVUS; PERO;

EID: 0033616797     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.96.7.4192     Document Type: Article

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