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4 spermine (60 μM), and 0.4% biocytin. When indicated, 10 or 30 mM BAPTA was added to the internal solution. Stimulating electrodes (patch pipettes filled with artificial cerebrospinal fluid (ACSF) or monopolar platinum iridium electrodes) were positioned in the CA3 pyramidal cell layer. Electrical stimuli (10 to 100 μA) were delivered every 3 or 6 s. Synaptic responses were filtered at 3 kHz and digitized at 30 kHz. Input and series resistances were monitored periodically; only cells with stable series and input resistances were used for the study. All drugs were dissolved in known quantities of ACSF and delivered through the bath perfusion system. After recording, all slices containing biocytin-filled interneurons were retained for morphological identification of the recorded neuron as described (31).
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Supported by the NIH (R.D.), the American Epilepsy Society (J.D.), and Sigma XI (F.L). We thank R. Calabrese and D. Jaeger for Neurolucida software, S. F. Traynelis for npm52 software, D. Leander for LY303070, D. Schoepp and J. Conn for LY341495, and A. Young for brain sections hybridized in situ for mGluR7 mRNA. We thank J. Conn, D. Mott, S. Traynelis, C McBain, and G. Maccaferri for useful comments on the manuscript.
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