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9 plaque-forming units per milliliter) (24) and placed on collagen gels. After 12 hours vehicle or 200 pM TGF-β2 was added and each explant analyzed as above after 24 hours. Cells were identified as virally infected by AMV3C2 antibody staining or alkaline phosphatase activity (25). The number of infected mesenchymal cells was divided by the total number of infected cells. All mean comparisons by Student's t test.
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We thank R. Emeson, A. George, L. Limbird, B. Hogan, C. Wright, and members of the Barnett laboratory for critical reading of the manuscript, M. Cleggett for technical assistance, C. Cepko for RCASBP containing alkaline phosphatase, and H. Moses for thoughtful discussion. Supported by grants HL52922 (J.V.B.), 38649 (R.B.R.), 42266 (R.B.R.), and FY98-0446 from the March of Dimes Birth Defects Foundation (J.V.B.), C.B.B. was supported by an institutional training grant from the NIH General Medical Sciences number GM07628. J.V.B. and R.B.R. are Established Investigators of the American Heart Association. J.V.B. wishes to acknowledge the support of the Vanderbilt Cancer Center, NCI CA68485.
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