Three-dimensional reconstruction of Lumbricus terrestris hemoglobin at 22 Å resolution: Intramolecular localization of the globin and linker chains

Journal of Molecular Biology

Volumn 289, Issue 5, 1999, Pages 1343-1359

  Taveau, Jean Christophe ^a   Boisset, Nicolas ^a   Vinogradov, Serge N ^b   Lamy, Jean N ^a  

^a UNIVERSITÉ DE TOURS   (France)

^b WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cryoelectron microscopy; Frozen hydrated specimens; Hemoglobin; Lumbricus terrestris; Three dimensional reconstruction

Indexed keywords

GLOBIN; HEMOGLOBIN; OLIGOMER; PROTEIN SUBUNIT;

ARTICLE; CRYOELECTRON MICROSCOPY; EARTHWORM; MODEL; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN TERTIARY STRUCTURE;

LUMBRICUS TERRESTRIS; PHERETIMA SIEBOLDI; STAPHYLOCOCCUS PHAGE 3A;

EID: 0033603389     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.2824     Document Type: Article

References (28)

1. Adrian M., Dubochet J., Lepault J., McDowall A. W. Cryoelectron microscopy of viruses. Nature. 308:1984;32-36.
2. Boekema E. J., Van Heel M. Molecular shape of Lumbricus terrestris erythrocruorin studied by electron microscopy and image analysis. Biochim. Biophys. Acta. 957:1989;370-379.
3. Boisset N., Penczek P. A., Taveau J. C., You V., de Haas F., Lamy J. Overabundant single-particle electron microscope views induce a three-dimensional reconstruction artifact. Ultramicroscopy. 74:1998;201-207.
4. de Haas F., Taveau J. C., Boisset N., Lambert O., Vinogradov S. N., Lamy J. N. Three-dimensional reconstruction of the chlorocruorin of the polychaete annelid Eudistylia vancouverii. J. Mol. Biol. 255:1996a;140-163.
5. de Haas F., Boisset N., Taveau J. C., Lambert O., Vinogradov S. N., Lamy J. N. Three-dimensional reconstruction of Macrobdella decora (Leech) hemoglobin by cryoelectron microscopy. Biophys. J. 70:1996b;1973-1984.
6. de Haas F., Zal F., Lallier F. H., Toulmond A., Lamy J. N. Three-dimensional reconstruction of the hexagonal bilayer hemoglobin of the hydrothermal vent tube worm Riftia pachyptila by cryoelectron microscopy. Proteins: Struct. Funct. Genet. 26:1996c;241-256.
7. de Haas F., Zal F., You V., Lallier F. H., Toulmond A., Lamy J. N. Three-dimensional reconstruction by cryoelectron microscopy of the giant hemoglobin of the polychaete worm Alvinella pompejana. J. Mol. Biol. 264:1996d;111-120.
8. de Haas F., Kuchumov A., Taveau J. C., Boisset N., Vinogradov S. N., Lamy J. N. Three-dimensional reconstruction of native and reassembled Lumbricus terrestris extracellular hemoglobin. Localization of the monomeric globin chains. Biochemistry. 36:1997;7330-7338.
9. Dubochet J., Adrian M., Chang J.-J., Homo J.-C., Lepault J., McDowall A. W., Schultz P. Cryoelectron microscopy of vitrified specimens. Quart. Rev. Biophys. 21:1988;129-228.
10. Felsenstein J. PHYLIP-phylogeny inference package (version 3. 2). Cladisties. 5:1989;164-166.
11. Frank J., Radermacher M., Penczek P., Zhu J., Li Y., Ladjadj M., Leith A. Spider and web: processing and visualization of images in 3D electron microscopy and related fields. J. Struct. Biol. 116:1996;190-199.
12. Harauz G., van Heel M. Exact filters for general geometry three-dimensional reconstruction. Optik. 73:1986;146-156.
13. Henderson R. Image contrast in high-resolution electron microscopy of biological macromolecules. TMV in ice. Ultramicroscopy. 46:1992;1-10.
14. Lambert O., Boisset N., Taveau J. C., Préaux G., Lamy J. N. Three-dimensional reconstruction of the αD- and βC-hemocyanins of Helix pomatia from frozen-hydrated specimens. J. Mol. Biol. 248:1995;431-448.
15. Martin P. D., Kuchumov A. R., Green B. N., Oliver R. W. A., Braswell E. H., Wall J. S., Vinogradov S. N. Mass spectrometry composition and molecular mass of Lumbricus terrrestris hemoglobin: a refined model of its quaternary structure. J. Mol. Biol. 255:1996;154-169.
16. Milligan R. A., Brisson A., Unwin P. N. T. Molecular structure determination of crystalline specimens in frozen aqueous solutions. Ultramicroscopy. 13:1984;1-10.
17. Orlova E. V., Dube P., Harris J. R., Beckman E., Zemlin E., Markl J., van Heel M. Structure of keyhole limpet hemocyanin type 1 (KLH1) at 15 Å resolution by electron cryomicroscopy and angular reconstruction. J. Mol. Biol. 271:1997;417-437.
18. Ownby D. W., Zhu H., Schneider K., Beavis B. T., Chait B. T., Riggs A. F. The extracellular hemoglobin of the earthworm Lumbricus terrestris. Determination of subunit stoichiometry. J. Biol. Chem. 268:1993;13539-13547.
19. Penczek P. Appendix: measures of resolution using Fourier Shell Correlation. J. Mol. Biol. 280:1998;115-116.
20. Penczek P., Radermacher M., Frank J. Three-dimensional reconstruction of single particles embedded in ice. Ultramicroscopy. 40:1992;33-53.
21. Penczek P. A., Grassucci R. A., Frank J. The ribosome at improved resolution: new techniques for merging and orientation refinement in 3D cryoelectron microscopy of biological material. Ultramicroscopy. 53:1994;251-253.
22. Royer W. E. Jr, Hendrickson W. A., Love W. Crystals of Lumbricus erythrocruorin. J. Mol. Biol. 197:1987;149-153.
23. Saxton W. O., Baumeister W. The correlation averaging of a regularly arranged bacterial envelope protein. J. Microscop. 127:1982;127-138.
24. Schatz M., Orlova E. V., Dube P., Jaeger J., Van Heel M. Structure of Lumbricus terrestris hemoglobin at 30 Å resolution determined using angular reconstruction. J. Struct. Biol. 114:1995;28-40.
25. Taveau J. C. Presentation of the Sigma software: Software of Imagery and Graphics for Molecular Architecture. J. Struct. Biol. 116:1996;223-229.
26. Vinogradov S. N., Sharma P. K. Preparation and characterization of invertebrate globin complexes. Methods Enzymol. 231:1994;112-124.
27. Vinogradov S. N., Lugo S. D., Mainwaring M. G., Kapp O. H., Crewe A. V. Bracelet protein: a quaternary structure proposed for the giant extracellular hemoglobin of Lumbricus terrestris. Proc. Natl Acad. Sci. USA. 83:1986;8034-8038.
28. Zhu H., Ownby D. W., Riggs C. K., Nolasco N. J., Stoops J. K., Riggs A. F. Assembly of the gigantic hemoglobin of the earthworm Lumbricus terrestris. J. Biol. Chem. 271:1996;30007-300021.