The lack of gonadotrophin-releasing hormone (GnRH) receptor desensitisation in αT3-1 cells is not due to GnRH receptor reserve or phosphatidylinositol 4,5-bis-phosphate pool size

Molecular and Cellular Endocrinology

Volumn 147, Issue 1-2, 1999, Pages 161-173

  Forrest Owen, W ^a   Willars, G B ^b   Nahorski, S R ^b   Assefa, D ^c   Davidson, J S ^c   Hislop, J ^a   McArdle, C A ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b UNIVERSITY OF LEICESTER   (United Kingdom)

^c UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Desensitization; G protein coupled receptor; Gonadotrophin releasing hormone; Intracellular calcium; Muscarinic receptor

Indexed keywords

CALCIUM ION; GONADORELIN RECEPTOR; GUANINE NUCLEOTIDE BINDING PROTEIN; MUSCARINIC RECEPTOR; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; PHOSPHOLIPASE C;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL TYPE; CONTROLLED STUDY; ENZYME ACTIVATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; RECEPTOR DOWN REGULATION;

ANIMALS; BUSERELIN; CALCIUM; CELL LINE; DOWN-REGULATION; ENDOTHELIN-1; GONADOTROPIN-RELEASING HORMONE; GTP-BINDING PROTEINS; HUMANS; INOSITOL PHOSPHATES; KINETICS; LITHIUM CHLORIDE; METHACHOLINE CHLORIDE; MICE; OXYTOCIN; PHOSPHATIDYLINOSITOL 4,5-DIPHOSPHATE; PHOSPHOLIPASE C; PHOTOAFFINITY LABELS; RECEPTOR, MUSCARINIC M3; RECEPTORS, CELL SURFACE; RECEPTORS, LHRH; RECEPTORS, MUSCARINIC; URIDINE TRIPHOSPHATE;

EID: 0033601852     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0303-7207(98)00201-9     Document Type: Article

References (44)

1. Akerman S.N., Zorec R., Cheek T.R., Moreton R.B., Berridge M.J., Mason W.T. Fura-2 imaging of thyrotropin-releasing hormone and dopamine effects on calcium homeostasis of bovine lactotrophs. Endocrinology. 129:1991;475-488.
2. Anderson L., McGregor A., Cook J.V., Chilvers E., Eidne K.A. Rapid desensitization of GnRH-stimulated intracellular signalling events in αT3-1 and HEK-293 cells expressing the GnRH receptor. Endocrinology. 136:1995;5228-5231.
3. Assefa, D., Pawson, A., McArdle, C.A., Forrest-Owen, W., Millar, R.P., Flanagan, C.A., Roeske, R., Davidson, J.S., 1998, in preparation.
4. Barbieri R.L. Clinical applications of GnRH and its analogues. Trends Endocrinol. Metab. 3:1992;30-34.
5. 3H]inositol 1,4,5-trisphosphate binding sites in adrenal-cortical membranes; characterization and validation of radioreceptor assay. Biochem. J. 265:1990;421-427.
6. Chen Z.-P., Kratzmeier M., Poch A., Xu S., McArdle C.A., Levy A., Mukhopadhyay A.K., Lightman S. Effects of extra-cellular nucleotides in the pituitary: ATP receptor mediated intra-cellular responses in gonadotrope-derived αT3-1 cells. Endocrinology. 137:1996;248-256.
7. Dohlman H.G., Thorner J., Caron M.G., Lefkowitz R.J. Model systems for the study of seven transmembrane segment receptors. Ann. Rev. Biochem. 60:1991;653-688.
8. Davidson J.S., Wakefield I.K., Millar R.P. Absence of rapid desensitization of the mouse gonadotropin-releasing hormone receptor. Biochem. J. 300:1994;299-302.
9. Evans J.J., McArdle C.A. Evidence for stimulation of phosphoinositidase C-linked intracellular processes by oxytocin and αT3-1 cells, a gonadotrope-derived cell line. Endocrinology. 138:1997;2049-2055.
10. Falck-Pedersen E., Heinflink M., Alvira M., Nussenzveig D.R., Gershengorn M.C. Expression of thyrotropin-releasing hormone receptors by adenovirus-mediated gene transfer reveals that thyrotropin-releasing hormone desensitization is cell-specific. Mol. Endocrinol. 45:1994;684-689.
11. Freedman N.J., Ament A.S., Oppermann M., Stoffel R.H., Exum S.T., Lefkowitz R.J. Phosphorylation and desensitization of human endothelin A and B receptors; evidence for G-protein-coupled receptor kinase specificity. J. Biol. Chem. 272:1997;17734-17743.
12. Hille B., Tse A., Tse F.W., Bosma M.M. Signaling mechanisms during the response of pituitary gonadotropes to GnRH. Rec. Prog. Horm. Res. 50:1995;75-95.
13. Huckle W.R., Conn P.M. The relationship of gonadotropin releasing hormone-stimulated luteinizing hormone release and inositol phosphate production: studies with calcium antagonists and protein kinase C activators. Endocrinology. 120:1987;160-169.
14. Jasper J.R., Harrell C.M., O'Brien J.A., Pettibone D.J. Characterization of the human oxytocin receptor stably expressed in 293 human embryonic kidney cells. Life Sci. 57:1995;2253-2261.
15. 1 muscarinic receptors. Mol. Pharmacol. 46:1994;1138-1148.
16. Jinnah H.A., Conn P.M. GnRH-mediated desensitization of cultured rat anterior pituitary cells can be uncoupled from LH release. Endocrinology. 118:1986;2599-2604.
17. Kaiser U.B., Conn P.M., Chin W.W. Studies of gonadotropin-releasing hormone (GnRH) action using GnRH receptor-expressing pituitary cell lines. Endocr. Rev. 18:1997;46-70.
18. Heding A., Vrecl M., Bogerd J., McGregor A., Sellar R., Taylor P.L., Eidne K.A. Gonadotropin-releasing hormone receptors with intracellular carboxyl-terminal tails undergo acute desensitization of total inositol phosphate production and exhibit accelerated internalization kinetics. J. Biol. Chem. 273:1998;11472-11477.
19. Lefkowitz R.J., Hausdorff W.P., Caron M.G. Role of phosphorylation in desensitization of the β-adrenoceptor. Trends Pharmacol. Sci. 11:1990;190-194.
20. 2+ in the αT3-1, gonadotrope-derived cell line. Mol. Cell Neurosci. 3:1992a;124-132.
21. McArdle C.A., Forrest-Owen W. Effects of pituitary adenylate cyclase activating polypeptide (PACAP) desensitize in αT3-1 gonadotrophs. J. Neuroendocrinol. 9:1997;893-902.
22. McArdle C.A., Forrest Owen W., Willars G., Davidson J., Poch A., Kratzmeier M. Desensitization of gonadotropin-releasing hormone action in the gonadotrope-derived αT3-1 cells line. Endocrinology. 136:1995;4864-4871.
23. McArdle C.A., Gorospe W.C., Huckle W.R., Conn P.M. Homologous down-regulation of gonadotropin releasing hormone receptors and desensitization of gonadotropes: lack of dependence on protein kinase C. Mol. Endocrinol. 1:1987;420-429.
24. McArdle C.A., Schomerus E., Gröner I., Poch A. Estradiol regulates gonadotropin-releasing hormone receptor number, growth, and inositol phosphate production in αT3-1 cells. Mol. Cell Endocrinol. 87:1992b;95-103.
25. 2+ mobilization. J. Biol. Chem. 271:1996;23711-23717.
26. Neill J.D., Duck L.W., Sellers J.C., Musgrove L.C., Scheschonka A., Druey K.M., Kehrl J.H. Potential role for a regulator of G-protein signaling (RGS3) in gonadotropin-releasing hormone (GnRH) stimulated desensitization. Endocrinology. 138:1997;843-846.
27. Neill J.D., Duck L.W., Musgrove L.C., Sellers J.C. Potential regulatory roles for G-protein-coupled receptor kinases and beta-arrestins in gonadotropin-releasing hormone receptor signaling. Endocrinology. 139:1998;1781-1788.
28. Neill J.D., Sellers J.C., Musgrove L.C., Duck W. Epitope-tagged gonadotropin-releasing hormone receptors heterologously-expressed in mammalian (COS-1) and insect (Sf9) cells. Mol. Cell Endocrinol. 127:1997;143-154.
29. Pratt, W.B., Taylor, P., 1990. Principles of Drug Action: The Basis of Pharmacology, 3rd ed., Churchill Livingstone, NY.
30. Sealfon S.C., Weinstein H., Millar R.P. Molecular mechanisms of ligand interaction with the gonadotropin-releasing hormone receptor. Endocrinol. Rev. 18(2):1997;180-205.
31. Stojilkovic S.S., Catt K.J. Expression and signal transduction pathways of endothelin receptors in neuroendocrine cells. Front Neuroendocrinol. 7:1996;327-369.
32. Stojilkovic S.S., Reinhart J., Catt K.J. Gonadotropin-releasing hormone receptors: structure and signal-transuction pathway. Endocrinol. Rev. 15:1994;462-499.
33. Tobin A.B. Phosphorylation of phospholipase C-coupled receptors. Phammacol. Ther. 75:1998;135-151.
34. Tobin A.B., Lambert D.G., Nahorski S.R. Rapid desensitization of muscarinic M3 receptor-stimulated polyphosphoinositide responses. Mol. Pharmacol. 42:1992;1042-1048.
35. Troskie, B.E., Sun, Y., Hapgood, J., Sealfon, S.C., Illing, N., Millar, R.P., 1997. Mammalian GnRH receptor functional features revealed by comparative sequences of goldfish, frog, and chicken receptors. Endocrine Society 79th Annual Meeting, Minneapolis, MN, abstract.
36. Tsutsumi M., Zhou W., Millar R.P., Mellon P.L., Roberts J.L., Flanagan C.A., Dong K., Gillo B., Sealfon S.C. Cloning and functional expression of a mouse gonadotropin-releasing hormone receptor. Mol. Endocrinol. 6:1992;1163-1169.
37. Wilkinson G.F., Purkiss J.R., Boarder M.R. Differential heterologous and homologous desensitization of two receptors for ATP (P2y purinoceptors and nucleotide receptors) coexisting on endothelial cells. Mol. Pharmacol. 45:1994;731-736.
38. Willars, G.B., McArdle, C.A., Nahorski, S.R., 1998a. Acute desensitization of phospholipase C-coupled muscarinic M3 - but not gonadotropin-releasing hormone (GnRH)-receptors co-expressed in αT3-1 cells - implications for mechanisms of rapid desensitization. Biochem. J., in press.
39. 2+ signalling in human neuroblastoma cells. Br. J. Pharmacol. 114:1995a;1133-1142.
40. 2+ store depletion? Mol. Pharmacol. 47:1995b;509-516.
41. Willars G.B., Nahorski S.R., Challis R.A.J. Differential regulation of muscarinic acetylcholine receptor-sensitive polyphosphoinositide pools and consequences for signaling in human neuroblastoma cells. J. Biol. Chem. 273:1998b;5037-5046.
42. Windle J.J., Weiner R.I., Mellon P.L. Cell lines of the pituitary gonadotrope lineage derived by targeted oncogenesis in transgenic mice. Mol. Endocrinol. 4:1990;597-603.
43. Wojcikiewicz R.J.H., Tobin A.B., Nahorski S.R. Desensitization of cell signalling mediated by phosphoinositidase C. Trends Pharmacol. Sci. 14:1993;279-285.
44. 2+ and by rapid desensitization. J. Neurochem. 63:1994;177-185.