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Volumn 121, Issue 11, 1999, Pages 2625-2626

Observation of ligand-based redox chemistry at the active site of a molybdenum enzyme [15]

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; FORMATE DEHYDROGENASE; LIGAND; MOLYBDENUM; OXIDOREDUCTASE;

EID: 0033599569     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9841761     Document Type: Letter
Times cited : (50)

References (22)
  • 2
    • 0000273676 scopus 로고    scopus 로고
    • (b) Hille, R. Chem. Rev. 1996, 96. 2757-2816.
    • (1996) Chem. Rev. , vol.96 , pp. 2757-2816
    • Hille, R.1
  • 8
    • 0032508380 scopus 로고    scopus 로고
    • VI trihydropterin species) have recently been detected in bacterial aldehyde dehydrogenases [Luykx, D. M. A.; Duine, J. A.; de Vries, S. Biochemistry 1998, 37, 11366-11375]. Although such species certainly indicate nonmetal-based redox chemistry, direct involvement of molybdenum ligands is not indicated.
    • (1998) Biochemistry , vol.37 , pp. 11366-11375
    • Luykx, D.M.A.1    Duine, J.A.2    De Vries, S.3
  • 9
    • 13044251016 scopus 로고    scopus 로고
    • 6 in 50 mM N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid buffer at pH 7.5 and frozen in 3 × 10 × 10 mm Lucite sample cuvettes. The enzyme was reduced with 10 mM sodium dithionite solution in the presence of 40 μM methyl viologen for 4 min. Dithionite was chosen in preference to formate as reductant because the latter gives rise to a mixture of oxidation states (analysis of EXAFS of mixtures can be problematic). Data were collected at a sample temperature of 10 K and 16 35-min sweeps were averaged for each sample.
  • 13
    • 13044283975 scopus 로고    scopus 로고
    • note
    • 2 are the Debye-Waller factors, and the values in parentheses are the estimated standard deviations (precisions) obtained from the diagonal elements of the covariance matrix.
  • 14
    • 13044270175 scopus 로고    scopus 로고
    • note
    • We note that EXAFS cannot readily distinguish between backscatterers of similar atomic number. For example, although Se-C and Se-S EXAFS are trivial to tell apart, Se-S and Se-Cl EXAFS are not. It is therefore possible that the selenosulfide sulfur observed in the Se EXAFS is in fact a chloride. We consider this possibility unlikely, as the samples were prepared in chloride-free buffer.
  • 16
    • 13044309961 scopus 로고    scopus 로고
    • note
    • We note that in this case the selenosulfide was retained in the dithionite-treated enzyme, although conditions of reduction differed in that no methyl viologen was added.
  • 20
    • 0000083402 scopus 로고    scopus 로고
    • III EPR studies of well-characterized complexes with ligands that are not biologically relevant [e.g., Pleune, B.; Poli, R.; Fettinger, J. C. J. Am. Chem. Soc. 1998, 120, 3257-3258].
    • (1998) J. Am. Chem. Soc. , vol.120 , pp. 3257-3258
    • Pleune, B.1    Poli, R.2    Fettinger, J.C.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.