Activation of a floral homeotic gene in Arabidopsis

Science

Volumn 285, Issue 5427, 1999, Pages 585-587

  Busch, Maximilian A ^a   Bomblies, Kirsten ^a   Weigel, Detlef ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARABIDOPSIS; ARTICLE; ENHANCER REGION; GENE ACTIVATION; GENE EXPRESSION REGULATION; HOMEOBOX; PLANT GROWTH; PRIORITY JOURNAL;

AGAMOUS PROTEIN, ARABIDOPSIS; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BINDING SITES; DNA-BINDING PROTEINS; ENHANCER ELEMENTS (GENETICS); GENE EXPRESSION REGULATION, PLANT; GENES, HOMEOBOX; GENES, PLANT; GENES, REPORTER; MERISTEM; PLANT COMPONENTS; PLANT PROTEINS; POINT MUTATION; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; TRANSCRIPTION FACTORS;

ARABIDOPSIS; EMBRYOPHYTA;

EID: 0033597938     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5427.585     Document Type: Article

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34. GUS fusions were in pDW294, a pCGN1547 derivative [K. E. McBride, K. R. Summerfelt, Plant Mol. Biol. 14, 269 (1990)] with the minimal -46-bp cauliflower mosaic virus promoter [P. N. Benfey and N.-H. Chua, Science 250, 959 (1990)] upstream of GUS [R. A. Jefferson, T. A. Kavanagh, M. W. Sevan, EMBO J. 6, 3901 (1987)]. Transgenic plants in the Columbia ecotype were generated by vacuum infiltration [N. Bechtold, J. Ellis, G. Pelletier, C.R. Acad. Sci. 316, 1194 (1993)]. GUS staining was as described [M. A. Blázquez, L. Soowal, I. Lee, D. Weigel, Development 124, 3835 (1997)].
35. GUS fusions were in pDW294, a pCGN1547 derivative [K. E. McBride, K. R. Summerfelt, Plant Mol. Biol. 14, 269 (1990)] with the minimal -46-bp cauliflower mosaic virus promoter [P. N. Benfey and N.-H. Chua, Science 250, 959 (1990)] upstream of GUS [R. A. Jefferson, T. A. Kavanagh, M. W. Sevan, EMBO J. 6, 3901 (1987)]. Transgenic plants in the Columbia ecotype were generated by vacuum infiltration [N. Bechtold, J. Ellis, G. Pelletier, C.R. Acad. Sci. 316, 1194 (1993)]. GUS staining was as described [M. A. Blázquez, L. Soowal, I. Lee, D. Weigel, Development 124, 3835 (1997)].
36. GUS fusions were in pDW294, a pCGN1547 derivative [K. E. McBride, K. R. Summerfelt, Plant Mol. Biol. 14, 269 (1990)] with the minimal -46-bp cauliflower mosaic virus promoter [P. N. Benfey and N.-H. Chua, Science 250, 959 (1990)] upstream of GUS [R. A. Jefferson, T. A. Kavanagh, M. W. Sevan, EMBO J. 6, 3901 (1987)]. Transgenic plants in the Columbia ecotype were generated by vacuum infiltration [N. Bechtold, J. Ellis, G. Pelletier, C.R. Acad. Sci. 316, 1194 (1993)]. GUS staining was as described [M. A. Blázquez, L. Soowal, I. Lee, D. Weigel, Development 124, 3835 (1997)].
37. GUS fusions were in pDW294, a pCGN1547 derivative [K. E. McBride, K. R. Summerfelt, Plant Mol. Biol. 14, 269 (1990)] with the minimal -46-bp cauliflower mosaic virus promoter [P. N. Benfey and N.-H. Chua, Science 250, 959 (1990)] upstream of GUS [R. A. Jefferson, T. A. Kavanagh, M. W. Sevan, EMBO J. 6, 3901 (1987)]. Transgenic plants in the Columbia ecotype were generated by vacuum infiltration [N. Bechtold, J. Ellis, G. Pelletier, C.R. Acad. Sci. 316, 1194 (1993)]. GUS staining was as described [M. A. Blázquez, L. Soowal, I. Lee, D. Weigel, Development 124, 3835 (1997)].
38. GUS fusions were in pDW294, a pCGN1547 derivative [K. E. McBride, K. R. Summerfelt, Plant Mol. Biol. 14, 269 (1990)] with the minimal -46-bp cauliflower mosaic virus promoter [P. N. Benfey and N.-H. Chua, Science 250, 959 (1990)] upstream of GUS [R. A. Jefferson, T. A. Kavanagh, M. W. Sevan, EMBO J. 6, 3901 (1987)]. Transgenic plants in the Columbia ecotype were generated by vacuum infiltration [N. Bechtold, J. Ellis, G. Pelletier, C.R. Acad. Sci. 316, 1194 (1993)]. GUS staining was as described [M. A. Blázquez, L. Soowal, I. Lee, D. Weigel, Development 124, 3835 (1997)].
39. D. R. Smyth, J. L. Bowman, E. M. Meyerowitz, Plant Cell 2, 755 (1990).
40. Electromobility shift assays were performed with purified His-tagged LFY protein (10).
41. We are grateful to T. Nguyen for technical assistance. We thank M. Blázquez, M. Deyholos, R. Hong, K. Jones, O. Nilsson, F. Parcy, L. Sieburth, D. Wagner, and M. Yanofsky for discussion, review of the manuscript, and communicating unpublished results. Supported by a fellowship from the Human Frontier Science Program Organization (M.B.), and grants from the NSF and Department of Energy (D.W.). D.W. is a NSF Young Investigator.