A convenient, solid-phase coupling of rhodamine dye acids to 5' amino-oligonucleotides

Tetrahedron Letters

Volumn 40, Issue 43, 1999, Pages 7611-7613

  Vinayak, Ravi ^a  

^a APPLIED BIOSYSTEMS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE; RHODAMINE;

ARTICLE; CHEMICAL MODIFICATION; DRUG SYNTHESIS; MOLECULAR INTERACTION; SOLID;

EID: 0033595864     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(99)01556-7     Document Type: Article

References (8)

1. Beaucage, S. L.; Iyer, R. P. Tetrahedron 1993, 49, 6123-6194.
2. Goodchild, J. Bioconjugate Chemistry 1990, 1, 165-187.
3. Theisen, P.; McCollum, C.; Upadhya, K.; Jacobson, K.; Vu, H.; Andrus, A. Tetrahedron Lett. 1992, 5033-5036; Theisen, P.; McCollum, C.; Andrus, A. Nucleic Acid Symposium Series; Oxford University Press: Oxford, UK, 1992; Vol. 27, pp. 99-100.
4. Theisen, P.; McCollum, C.; Upadhya, K.; Jacobson, K.; Vu, H.; Andrus, A. Tetrahedron Lett. 1992, 5033-5036; Theisen, P.; McCollum, C.; Andrus, A. Nucleic Acid Symposium Series; Oxford University Press: Oxford, UK, 1992; Vol. 27, pp. 99-100.
5. Mullah, B.; Andrus, A. Tetrahedron Lett. 1997, 5751-5754.
6. van der Laan, A. C.; Brill, R.; Kuimelis, R. G.; Kuyl-Yeheskiely, E.; van Boom, J. H.; Andrus, A.; Vinayak, R. Tetrahedron Lett. 1997, 2249-2252.
7. 2O (1:1:2) for 3 h at 65°C; Ref. 4 and US Patent. No. 4,965,349. The oligonucleotides were purified using RP-HPLC gradient as described in Ref. 7.
8. 3CN) with a flow rate of 1.0 ml/min. The used gradient was 15% B to 35% B in 30 min.