EPR detection of the transient tyrosyl radical in DNA photolyase from Anacystis nidulans [8]

Journal of the American Chemical Society

Volumn 121, Issue 37, 1999, Pages 8659-8660

  Aubert, Corinne ^a   Brettel, Klaus ^a   Mathis, Paul ^a   Eker, André P M ^b   Boussac, Alain ^a  

^a CEA SACLAY   (France)

^b ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBODIPYRIMIDINE PHOTOLYASE; RADICAL; TYROSINE;

ALGA; CHEMICAL REACTION KINETICS; ELECTRON SPIN RESONANCE; ELECTRON TRANSPORT; ENZYME ACTIVATION; LETTER; NONHUMAN; PHOTOACTIVATION; PHYSICAL CHEMISTRY;

EID: 0033595465     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja991938z     Document Type: Letter

References (19)

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2. Stubbe, J.; van der Donk, W. A. Chem. Rev. 1998, 98, 705-762. Very recently, a tyrosyl radical has also been found in cytochrome c oxidase: MacMillan, F.; Kannt, A.; Behr, J.; Prisner, T.; Michel, H. Biochemistry 1999, 38, 9179-9184.
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8. -.
9. Implications of this kinetic isotope effect for the reaction mechanism will be discussed in a forthcoming paper. For a recent treatise of isotope effects on enzymatic reactions, see: Cook, P. F., Ed. Enzyme Mechanism from Isotope Effects; CRC Press: Boca Raton, FL, 1991.
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13. The tryptophanyl radical is too short-lived to be detected at our instrumental time resolution of 10 ms.
14. • spectrum when the excitation flashes were stopped).
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