Negative selection driven by cytosine deaminase gene in Lycopersicon esculentum hairy roots

Plant Science

Volumn 141, Issue 2, 1999, Pages 175-181

  Hashimoto, R Y ^a   Menck, C F M ^b   Van Sluys, M A ^a  

^a R do Matao 277 05508 900 Cd U   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Cytosine deaminase gene; Hairy root clones; Negative selection; Tomato

Indexed keywords

CYTOSINE DEAMINASE;

ARTICLE; GENE EXPRESSION; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; PLANT ROOT; TOMATO; TRANSGENIC PLANT;

AGROBACTERIUM RHIZOGENES; LYCOPERSICON ESCULENTUM;

EID: 0033593783     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-9452(98)00242-8     Document Type: Article

References (20)

1. Mullen C.A., Kilstrup M., Blaese R.M. Transfer of the bacterial gene for cytosine deaminase to mammalian cells confers lethal sensitivity to 5-fluorocytosine: A negative selection system. Proc. Natl. Acad. Sci. USA. 89:1992;33-37.
2. Béclin C., Charlot F., Botton E., Jouanin L., Doré C. Potential use of aux2 gene from Agrobacterium rhizogenes as a conditional negative marker in transgenic cabbage. Transgenic Res. 2:1993;48-55.
3. Miao Z.H., Lam E. Targeted disruption of the TGA3 locus in Arabidopsis thaliana. Plant J. 7:1995;359-365.
4. Perera R.J., Linard C.G., Singer E.R. Cytosine deaminase as a negative selective marker for Arabidopsis. Plant Mol. Biol. 23:1993;793-799.
5. Kobayashi T., Hisajima S., Stougaard J., Ichikawa H. A conditional negative selection for Arabibopsis expressing a bacterial cytosine deaminase gene. Jpn. J. Genet. 70:1995;409-422.
6. Thykjaer T., Finnemann J., Schauser L., Christensen L., Poulsen C., Stougaard J. Gene targeting approaches using positive-negative selection and large flanking regions. Plant Mol. Biol. 35:1997;523-530.
7. Riseeuw E., Franke-van Dijk M. E., Hooykaas P.J. Gene targeting and instability of Agrobacterium T-DNA loci in the plant genome. Plant J. 11:1997;717-728.
8. Danielsen S., Kilstrup M., Barilla K., Jochimsen B., Neuhard J. Characterization of the Escherichia coli codBA operon encoding cytosine permease and cytosine deaminase. Mol. Microbiol. 6:1992;1335-1344.
9. Damon L.E., Cadwin E., Benz C. Enhancement of 5-fluorouracil antitumor effects by the prior administration of methotrexate. Pharmacol. Ther. 43:1989;155-185.
10. Tempé J., Casse-Delbart F. Plant gene vectors and genetic transformation: Agrobacterium Ri plasmids. Schell J., Indra V. Molecular Biology of Nuclear Genes. 1989;25-49 Academic Press, New York.
11. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd edn:1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
12. Gynheung A., Ebert P.R., Mitra A., Há S.B. Binary vectors. Gelvin S.B., Schilperoort P.A., Verma D.P.S. Plant Gene Research Manual. 1989;1-27 Martinus Nijhoff, Dordrecht.
13. Ditta G., Stanfield S., Corbin D., Helinski D.R. Broad hoot range DNA cloning system from Gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA. 12:1980;7347-7351.
14. Petit A., Tempé J. Isolation of Agrobacterium Ti-plasmid regulatory mutants. Mol. Gen. Genet. 167:1978;147-155.
15. Murashige T., Skoog F.A. A revised medium for rapid growth and bioassays with Tobacco tissue cultures. Physiol. Plant. 15:1962;473-497.
16. Mettler I.J. A simple and rapid method for minipreparation of DNA from tissue cultured plant cells. Plant Mol. Biol. Rep. 5:1989;346-349.
17. Van Sluys M.A., Tempé J. Behavior of the maize transposable element Activator in Daucus carota. Mol. Gen. Genet. 219:1989;313-319.
18. Sukhapinda K., Spivey R., Simpson R.B., Shahin E.A. Transgenic tomato (Lycopersicon esculentum L.) transformed with a binary vector in Agrobacterium rhizogenes: non-chimeric origin of callus clone and low copy numbers of integrated vector T-DNA. Mol. Gen. Genet. 206:1987;491-497.
19. Schlaman H.R.M., Hooykaas P.J.J. Effectiveness of the bacterial gene codA encoding cytosine deaminase as a negative selectable marker in Agrobacterium-mediated plant transformation. Plant J. 11:1997;1377-1385.
20. Liljegren S.J., Yanofsky M.F. response: Targeting Arabidopsis. Trends Plant Sci. 3(3):1998;79-80.