Transformed lepidopteran insect cells: New sources of recombinant human tissue plasminogen activator

Biotechnology and Bioengineering

Volumn 64, Issue 4, 1999, Pages 426-433

  Farrell, Patrick J ^a   Behie, Leo A ^a   Iatrou, Kostas ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

Cell transformation; Insect cells; Protein expression; Recombinant proteins; Secreted glycoproteins; Signal peptides; Silkmoth cells; Tissue plasminogen activator

Indexed keywords

ANIMAL CELL CULTURE; CELLS; CLONING; DNA; ENZYMES; GENES; MOLECULAR WEIGHT; PROTEINS; TISSUE;

CELL TRANSFORMATION; INSECT CELLS; PROTEIN EXPRESSION; RECOMBINANT PROTEINS; SECRETED GLYCOPROTEINS; SIGNAL PEPTIDES; SILKMOTH CELLS; TISSUE PLASMINOGEN ACTIVATOR;

GENETIC ENGINEERING;

ALTEPLASE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CELL TRANSFORMATION; CHORION; INSECT; NONHUMAN; PLASMID; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN SECRETION; RELATIVE DENSITY;

ANIMALIA; INSECTA; LEPIDOPTERA;

EID: 0033588303     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1097-0290(19990820)64:4<426::aid-bit5>3.0.co;2-%23     Document Type: Article

References (33)

1. Boose JA, Kuismanen E, Gerard R, Sambrook J, Gething M-J. 1989. The single-chain form of tissue-type plasminogen activator has catalytic activity: Studies with a mutant enzyme that lacks the cleavage site. Biochemistry 28:635-643.
2. Collen D, Lijnen HR. 1986. The fibrinolytic system of man. Crit Rev Oncol Hematol 4:249-301.
3. Datar RV, Cartwright T, Rosen C-G. 1993, Process economics of animal cell and bacterial fermentations: a case study analysis of tissue plasminogen activator. Bio/Technology 11:351-357.
4. Davis TR, Wood HA. 1995. Intrinsic glycosylation potentials of insect cell cultures and insect larvae. In Vitro Cell Dev Biol 31:659-663.
5. Dee KU, Shuler ML, Wood HA.. 1997. Inducing single-cell suspension of BTI-TN-5B1-4 insect cells. I. The use of sulfated polyanions to prevent cell aggregation and enhanced recombinant protein production. Biotechnol Bioeng 54:191-205.
6. Farrell PJ, Lu M, Prevost J, Brown C, Behie LA, Iatrou K. 1998. High-level expression of secreted glycoproteins in transformed lepidopteran insect cells using a novel expression vector. Biotechnol Bioeng 60: 656-663.
7. Feingberg AP, Vogelstein B. 1983. A technique for radiolabeling DNA restricted endonuclease fragments to high specific activity. Anal Biochem 132:6-13.
8. Fotaki ME, Iatrou K. 1988. Identification of a transcriptionally active pseudogene in the chorion locus of the silkmoth Bombyx mori. J Mol Biol 203:849-860.
9. Grace TDC. 1967. Establishment of a line of cells from the silkworm Bombyx mori. Nature 216:613.
10. Harris RJ, Leonard CK, Guzetta AW, Spellman MW. 1991. Tissue plasminogen activator has an O-linked fucose attached to threonine-61 in the epidermal growth factor domain. Biochemistry 30:2311-2314.
11. Hippenmeyer P, Highkin M. 1993. High level, stable production of recombinant proteins in mammalian cell culture using the herpesvirus VP16 transactivator. Bio/Technology 11:1037-1041.
12. Hippenmeyer PJ, Pegg LE. 1995. Enhancing expression of recombinant proteins in mammalian cells using the herpes virus VP16 transactivator. Curr Opin Biotechnol 6:548-552.
13. Ichinose A, Kisiel W, Fujikawa K. 1984. Proteolytic activation of tissue plasminogen activator by plasma and tissue enzymes. FEBS Lett 175: 412-418.
14. Jarvis DL, Fleming J-AGW, Kovacs GR, Summers MD, Guarino LA. 1990. Use of early baculovirus promoters for continuous expression and efficient processing of foreign gene products in stably transformed lepidopteran cells. Bio/Technology 8:950-955.
15. Jarvis DL, Summers MD. 1989. Glycosylation and secretion of human tissue plasminogen activator in recombinant baculovirus-infected cells. Mol Cell Biol 9:214-223.
16. Jarvis DL, Summers MD, Garcia A, Jr, Bohlmeyer DA. 1993. Influence of different signal peptides and prosequences on expression and secretion of human tissue plasminogen activator in the baculovirus system. J Biol Chem 268(22): 16754-16762.
17. Jarvis DL, Weinkauf C, Guarino LA. 1996. Immediate-early baculovirus vectors for foreign gene expression in transformed or infected insect cells. Prot Express Purif 8:191-203.
18. Johnson R, Meidinger RG, Iatrou K. 1992. A cellular promoter-based expression cassette for generating recombinant baculoviruses directing rapid expression of passenger genes in infected insects. Virology 190: 815-823.
19. Lemontt JF, Wei C-M, Dackowski WR. 1985. Expression of human uterine tissue plasminogen activator in yeast. DNA 4:419-428.
20. Lu M, Johnson RR, Iatrou K. 1996. Trans-Activation of a cell housekeeping gene promoter by the IE1 gene product of baculoviruses. Virology 218:103-113.
21. Lu M, Farrell PJ, Johnson R, Iatrou K. 1997. A baculovirus (BmNPV) repeat element functions as a powerful constitutive enhancer in transfected insect cells. J Biol Chem 272:30724-30728.
22. Martegani E, Forlani N, Mauri I, Porro D, Schleuning WD, Alberghina L. 1992. Expression of high levels of human tissue plasminogen activator in yeast under the control of an inducible GAL promoter. Appl Microbiol Biotechnol 37:604-608.
23. Monroe TJ, Muhlmann-Diaz MC, Kovach MJ, Carlson JO, Bedford JS, Beaty BJ. 1992. Stable transformation of a mosquito cell line results in extraordinarily high copy numbers of the plasmid. Proc Natl Acad Sci USA 89:5725-5729.
24. Murphy CA, McIntire JR, Davis DvR, Hodgdon H, Seals JR, Young E. 1993. Enhanced expression, and large-scale purification of recombinant HIV-1 gp120 in insect cells using the baculovirus egt and p67 signal peptides. Prot Express Purif 4:349-357.
25. Parekh RB, Dwek RA, Rudd PM, Thomas JR, Rademacher TW. 1989. N-glycosylation and in vitro enzymatic activity of human recombinant tissue plasminogen activator expressed in Chinese hamster ovary cells and a murine cell line. Biochemistry 28:7670-7679.
26. Pennica D, Holmes WE, Kohr WJ, Harkins RN, Vehar GA, Ward CA, Bennett WF, Yelverton E, Seeburg PH, Heyneker HL, Goeddel DV. 1983. Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature 301:214-221.
27. Pohl G, Källström M, Bergsdorf N, Wallen P, Jörnvall H. 1984. Tissue plasminogen activator: peptide analyses confirm an indirect amino acid sequence, identify the active site serine residue, establish glycosylation sites, and localize variant differences. Biochemistry 23: 3701-3707.
28. Spoerel N, Nguyen HT, Kafatos FC. 1986. Gene regulation and evolution in the chorion locus of Bombyx mori: structural and developmental characterization of four eggshell genes and their flanking DNA regions. J Mol Biol 190:23-35.
29. Sprengers ED, Kluft C. 1987. Plasminogen activator inhibitors. Blood 69:381-387.
30. Stavroulakis DA, Kalogerakis N, Behie LA, Iatrou K. 1991. Growth characteristics of a Bombyx mori insect cell line in stationary and suspension cultures. Can J Chem E 69:457-464.
31. Steiner H, Pohl P, Gunne H, Hellers M, Elhammer A, Hansson L. 1988. Human tissue-type plasminogen activator synthesized by using a baculovirus vector in insect cells compared with human plasminogen activator produced in mouse cells. Gene 73:449-457.
32. Tessier DC, Thomas DY, Khouri HE, Laliberte F, Vernet T. 1991. Enhanced secretion from insect cells of a foreign protein fused to the honeybee melittin signal peptide. Gene 98:177-183.
33. Upshall A, Kumar AA, Bailey MC, Parker MD, Favreau MA, Lewison KP, Joseph ML, Maraganore JM, McKnight GL. 1987. Secretion of active tissue plasminogen activator from the filamentous fungus Aspergillus nidulans. Bio/Technology 5:1301-1304.