Electrically immobilized enzyme reactors: Bioconversion of a charged substrate. Hydrolysis of penicillin G by penicillin G acylase

Biotechnology and Bioengineering

Volumn 64, Issue 4, 1999, Pages 383-391

  Bossi, Alessandra ^a   Guerrera, Stefania ^a   Righetti, Pier Giorgio ^a  

^a UNIVERSITY OF VERONA   (Italy)

Author keywords

Immobilized reactors; Isoelectric membranes; Penicillin G acylase

Indexed keywords

BIOLOGICAL MEMBRANES; BIOREACTORS; ELECTRIC FIELD EFFECTS; ELECTROPHORESIS; ENZYME IMMOBILIZATION; ENZYMES; HYDROLYSIS; IMPURITIES;

AMPHOTERIC NATURE; ELECTROLYZER; ELECTROPHORETIC TRANSPORT; ISOELECTRIC MEMBRANES; MULTICOMPARTMENT IMMOBILIZED ENZYME REACTOR; PENICILLIN ACYLASE;

BIOCONVERSION;

6 AMINOPENICILLANIC ACID; IMMOBILIZED ENZYME; PENICILLIN AMIDASE; PENICILLIN G; PHENYLACETIC ACID;

ARTICLE; BIOREACTOR; CATALYSIS; ELECTRIC FIELD; ENZYME ACTIVITY; ENZYME METABOLISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROLYSIS; PROTON TRANSPORT;

BIOREACTORS; BUFFERS; ELECTRICITY; ENZYMES, IMMOBILIZED; HYDROLYSIS; PENICILLIN AMIDASE; PENICILLIN G; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

PAA;

EID: 0033588008     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990820)64:4<383::AID-BIT1>3.0.CO;2-H     Document Type: Article

References (22)

1. Arnott IA, Weatherley LR. 1995. The stability of penicillin G during recovery by electrically enhanced extraction. Process Biochem 30:447-455.
2. Benedict RG, Schmidt WH, Coghill RD, Oleson AP. 1945. Penicillin. III. The stability of penicillin in aqueous solutions. J Bacteriol 49:85-95.
3. Blaha JM, Knevel AM, Kessler DP, Mincy JW, Hem SL. 1976. Kinetic analysis of penicillin G degradation in acidic media. J Pharm Sci 8:1165-1170.
4. Bossi A, Cretich M, Righetti PG. 1998. Production of D-phenylglycine from racemic (D,L)-phenylglycine via isoelectrically trapped penicillin G acylase. Biotechnol Bioeng 20:454-461.
5. Castagnola M, Messana I, Rossetti DV. 1996. Capillary zone electrophoresis of peptides. In: Righetti PG, editor. Capillary Electrophoresis in Analytical Biotechnology. Boca Raton: CRC Press. p 239-275.
6. Chiari M, Casale E, Santaniello E, Righetti PG. 1989a. Synthesis of buffers for generating immobilized pH gradients. I. Acidic acrylamido buffers. Appl Theor Electr 1:99-102.
7. Chiari M, Casale E, Santaniello E, Righetti PG. 1989b. Synthesis of buffers for generating immobilized pH gradients. II. Basic acrylamido buffers. Appl Theor Electr 1:103-107.
8. Chiari M, Dell'Orto N, Mendozza M, Carrea G, Righetti PG. 1996. Enzyme reactions in a multicompartment electrolyzer with isoelectrically trapped enzymes. J Biochem Biophys Methods 31:93-104.
9. Gelfi C, Orsi A, Leoncini F, Righetti PG. 1995. Fluidified polyacrylamides as molecular sieves in capillary zone electrophoresis of DNA fragments. J Chromatogr A 689:97-105.
10. Giaffreda E, Tonani C, Righetti PG. 1993. A pH gradient simulator for electrophoretic techniques in a windows environment. J Chromatogr 630:313-327.
11. Ishimura F, Suga KI. 1992. Hydrolysis of penicillin G by combination of immobilized penicillin G acylase and electrodialysis. Biotechnol Bioeng 39:171-175.
12. Nembri F, Bossi A, Ermakov S, Righetti PG. 1997. Isoelectrically trapped enzymatic bioreactors in a multimembrane cell coupled to an electric field: Theoretical modelling and experimental validation with urease. Biotechnol Bioeng 53:110-119.
13. Nidetzky B, Neuhauser W, Haltrich D, Kulbe KD. 1996. Continuous enzymatic production of xylitol with simultaneous coenzyme regeneration in a charged membrane reactor. Biotechnol Bioeng 52:387-396.
14. Righetti PG. 1983. Isoelectric focusing: Theory, methodology and applications. Amsterdam: Elsevier.
15. Righetti PG. 1990. Immobilized pH gradients: Theory and methodology. Amsterdam: Elsevier.
16. Righetti PG, Bossi A. 1998. An isoelectrically trapped enzyme reactor operating in an electric field. Electrophoresis 19:1075-1080.
17. Righetti PG, Delpech M, Moisand F, Kruh J, Labie D. 1984. Immobilized pH gradients for isoelectric focusing: Interaction between histones and histone-like proteins with the charged polyacrylamide matrix. Electrophoresis 4:393-398.
18. Righetti PG, Nembri F, Bossi A, Mortarino M. 1997. Continuous enzymatic hydrolysis of β-casein and isoelectric collection of some of the biologically active peptides in an electric field. Biotechnol Prog 13: 258-264.
19. Righetti PG, Wenisch E, Faupel M. 1989. Preparative protein purification in a multi-compartment electrolyzer with Immobiline membranes. J Chromatogr 475:293-309.
20. Righetti PG, Wenisch E, Jungbauer A, Katinger H, Faupel, M. 1990. Preparative purification of human monoclonal antibody isoforms in a multicompartment electrolyzer with Immobiline membranes. J Chromatogr 500:681-696.
21. Svensson H. 1962. Isoelectric fractionation, analysis and characterization of ampholytes in natural pH gradients. II. Properties of carrier ampholyte buffers. Acta Chem Scand 16:456-466.
22. Vesterberg O. 1969. Synthesis and isoelectric fractionation of carrier ampholytes. Acta Chem Scand 23:2653-2665.