Control of acetic acid concentration by pH-Stat continuous substrate feeding in heterotrophic culture phase of two-stage cultivation of Alcaligenes eutrophus for production of P(3HB) from CO2, H2, and O2 under non-explosive conditions

Biotechnology and Bioengineering

Volumn 62, Issue 6, 1999, Pages 625-631

  Sugimoto, Toshikazu ^a   Tsuge, Takeharu ^a   Tanaka, Kenji ^a   Ishizaki, Ayaaki ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Acetic acid; Alcaligenes eutrophus; Feed control; PHB; Substrate inhibition

Indexed keywords

ACETIC ACID; BACTERIA; CARBON DIOXIDE; FRUCTOSE; HYDROGEN; ORGANIC POLYMERS; OXYGEN; PH EFFECTS; SUBSTRATES;

AUTOTROPHIC CELL CULTURE; CELL INHIBITION; HETEROTROPHIC CELL CULTURE; POLYHYDROXYBUTYRATE;

BATCH CELL CULTURE;

ACETIC ACID; CARBON DIOXIDE; FRUCTOSE; HYDROGEN; LACTIC ACID; OXYGEN; POLY(3 HYDROXYBUTYRIC ACID);

ACIDITY; ARTICLE; BACTERIAL COUNT; BACTERIAL GROWTH; BACTERIUM CULTURE; BIOTECHNOLOGY; CONTROLLED STUDY; FERMENTATION; GROWTH INHIBITION; INCUBATION TIME; NONHUMAN; OXYGEN CONCENTRATION; REACTION ANALYSIS; SYNTHESIS; WAUTERSIA EUTROPHA;

ALCALIGENES; BACTERIA (MICROORGANISMS); BACTERIUM ALCALIGENES; NEGIBACTERIA; WAUTERSIA EUTROPHA;

EID: 0033586350     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990320)62:6<625::AID-BIT1>3.0.CO;2-D     Document Type: Article

References (30)

1. Allen RJL. 1940. The estimation of phosphorus. Biochem J 34:858-865.
2. Anderson AJ, Dawes EA. 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev 54:450-472.
3. Decicco BT, Stuccos PE. 1968. Autotrophic and heterotrophic metabolism of Hydrogenomonas. J Bacteriol 95:1469-1475.
4. Friedrich CG, Barbel F, Bowien B. 1981. Formation of enzymes of autotrophic metabolism during heterotrophic growth of Alcaligenes eutrophus. J Gen Microbiol 122:69-78.
5. Herbert D, Phipps J, Strange RE. 1971. Chemical analysis of microbial cells: Determination of protein. In: Morris JR and Ribbons DW. editors. Methods in Microbiology. New York: Academic Press. VoL 5, p 243-249.
6. Ikeda S, Ishizaki A. Hirose Y. 1973. Acid-base equilibrium in aerobic fermentations: Influence of un-utilizable acids and bases on the acetic acid concentration in culture liquid. Biotechnol Bioeng 15:1147-1158.
7. T using recycled gas closed circuit culture system. J Ferment Bioeng 69:170-174.
8. T. J Ferment Bioeng 69:170-174.
9. Ishizaki A, Ueda T, Tanaka K, Stanbury PF. 1992. L-Lactatc production from xylose employing Lactococcus lactis IO-1. Biotechnol Lett 14: 599-604.
10. Ishizaki A, Ueda T, Tanaka K, Stanbury PF. 1993. The kinetics of end product inhibition of i.-lactate production from xylose and glucose by Lactococcus lactis IO-1. Biotechnol Lett 15:489-494.
11. Kim BS, Lee SC, Lee SY, Chang HN, Chang YK, Woo SI. 1994. Production of poly(β-hydroxybutyric-co-β-hydroxyvaleric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control. Biotechnol Bioeng 43:892-898.
12. Kim SW, Kim P, Lee HS, Kim JH. 1996. High production of poly-ß-hydroxybutyrate (PHB) from Methylobacterium organophilum under potassium limitation. Biotechnol Lett 18:25-30.
13. Kuehn GD, Mcfadden BA. 1968. Factors affecting the synthesis and degradation of ribulose-1,5-diphosphate carboxylase in Hydrogenomonas facials and Hydrogenomonas eutropha. J Bacteriol 95:937-946.
14. Lee IY, Kim MK, Kim GJ, Chang HN, Park YH. 1995. Production of poly(β-hydroxybutyrate-co-β-hydroxyvalerate) from glucose and valerate in Alcaligenes eutrophus. Biotechnol Lett 17:571-574.
15. Luli GW, Schlasner SM, Ordaz DE, Mason M, Strohl WR. 1987. An automatic on-line glucose analyzer for feed control of fed-batch growth of Escherichia coli. Biotechnol Tech 1:225-230.
16. Mizutani S, Iijima S, Morikawa M, Shimizu K, Matsubara M, Ogawa Y, Izumi R, Matsumato K, Kobayashi T. 1987. On-line control of glucose concentration using an automatic glucose analyzer. J Ferment Technol 65:325-331.
17. Mori H, Yano T, Kobayashi T, Shimizu S. 1979. High density cultivation of biomass in fed-batch system with DO-stat. J Chem Eng Jpn 12: 313-319.
18. Park YS. 1996. Strategies for the enhancement of biological and metabolic activity in microorganism. Seibutsu-kogaku 74:171-183 (in Japanese).
19. Russell JA. 1944. The colorimetric estimation of small amounts of ammonia by the phenol-hypochlorite reaction. J Biol Chem 156:457-461.
20. Shimizu H, Tamura S, Shioya S, Suga K. 1993. Kinetic study of poly-D-3-hydroxybutyric acid (PHB) production and its molecular weight distribution control in a fed-batch culture of Alcaligenes eutrophus. J Ferment Bioeng 76:465-469.
21. Siegel RS, Ollis DF. 1984. Kinetics of growth of the hydrogen-oxidizing bacterium Alcaligenes eutrophus ATCC 17707 in chemostat culture. Biotechnol Bioeng 26:764-770.
22. Smolders GJF, Bulstra DJ, Jacobs R, van Loosdrecht MCM, Heijnen JJ. 1995. A metabolic model of the biological phosphorus removal process: II. Validation during start-up conditions. Biotechnol Bioeng 48: 234-245.
23. Stukus PE, Decicco BT. 1970. Autotrophic and heterotrophic metabolism of Hydrogenomonas: Regulation of autotrophic growth by organic substrate. J Bacteriol 101:339-345.
24. Suzuki T, Yamanc T, Shimizu S. 1990. Phenomenological background and some preliminary trials of automated supply in pH-stat modal fedbatch culture using a setpoint high limit. J Ferment Bioeng 69: 292-297.
25. Tags N, Tanaka K, Ishizaki A. 1997. Effects of Theological change by addition of carboxymethylcellulose in culture media of an air-lift fermenter on poly-D-3-hydroxybutyric acid productivity in autotrophic culture of hydrogen-oxidizing bacterium. Alcaligenes eutrophus. Biotechnol Bioeng 53:529-533.
26. T. J Ferment Bioeng 77:425-427.
27. 2 by high cell density autotrophic cultivation of Alcaligenes eutrophus. Biotechnol Bioeng 45:268-275.
28. Tanaka K, Katamune K, Ishizaki A. 1995b. Fermentative production of poly-β-hydroxybutyric acid from xylose via 1-lactate by a two-stage culture method employing Lactococcus lactis IO-1 and Alcaligenes eutrophus. Can J Microbiol 41:257-261.
29. Yamane T, Fukunaga M, Lee YW. 1996. Increased PHB productivity by high-cell-density fed-batch culture of Alcaligenes latus, a growth associated PHB producer. Biotechnol Bioeng 50:197-202.
30. Yano T, Mori H, Kobayashi T, Shimizu S. 1978. Fed-batch culture of methanol-utilizing bacterium with DO-stat. J Ferment Technol 56: 416-420.