Immunoelectron microscopy of AMPA receptor subunits reveals three types of putative glutamatergic synapse in the rat vestibular end organs

Brain Research

Volumn 819, Issue 1-2, 1999, Pages 58-64

  Matsubara, Atsushi ^a   Takumi, Yutaka ^a   Nakagawa, Takashi ^b   Usami, Shin Ichi ^a   Shinkawa, Hideichi ^a   Ottersen, Ole P ^c  

^a HIROSAKI UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

^c UNIVERSITY OF OSLO   (Norway)

Author keywords

Afferent; AMPA; Immunocytochemistry; Neurotransmitter; Rat; Vestibular end organ

Indexed keywords

AMPA RECEPTOR; GLUTAMIC ACID; RECEPTOR SUBUNIT;

ANIMAL TISSUE; ARTICLE; CELL ULTRASTRUCTURE; CONTROLLED STUDY; EMBEDDING; HAIR CELL; IMMUNOCYTOCHEMISTRY; IMMUNOELECTRON MICROSCOPY; NERVE ENDING; NONHUMAN; PRIORITY JOURNAL; RAT; SENSORY NERVE; SYNAPSE; VESTIBULE;

ANIMALS; GLUTAMINE; HAIR CELLS, VESTIBULAR; IMMUNOHISTOCHEMISTRY; MICROSCOPY, IMMUNOELECTRON; NEURONS, AFFERENT; PRESYNAPTIC TERMINALS; RATS; RECEPTORS, AMPA; SYNAPSES; VESTIBULAR NERVE; VESTIBULE;

EID: 0033585835     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-8993(98)01345-6     Document Type: Article

References (26)

1. Anniko M., Lundquist P.G. Temporal bone morphology after systemic arterial perfusion or intralabyrinthine in situ immersion: 1. Hair cells of the vestibular organs and the cochlea. Micron. 11:1980;73-83.
2. Baird R.A., Desmadryl G., Fernández C., Goldberg J.M. The vestibular nerve of the chinchilla: II. Relation between afferent response properties and peripheral innervation patterns in the semicircular canals. J. Neurophysiol. 60:1988;182-203.
3. Chaudhry F.A., Lehre K.P., von Lookeren Campagne M., Ottersen O.P., Danbolt N.C., Storm-Mathisen J. Glutamate transporters in glial plasma membranes: highly differentiated localizations revealed by quantitative ultrastructural immunocytochemistry. Neuron. 15:1995;711-720.
4. Demêmes D., Lleixa A., Dechesne C.J. Cellular and subcellular localization of AMPA-selective glutamate receptors in the mammalian peripheral vestibular system. Brain Res. 671:1995;83-94.
5. Demêmes D., Wenthold R.J., Moniot B., Sans A. Glutamate-like immunoreactivity in the peripheral vestibular system of mammals. Hearing Res. 46:1990;261-270.
6. Devau G., Lehouelleur J., Sans A. Glutamate receptors on type I vestibular hair cells of guinea-pig. Eur. J. Neurosci. 5:1993;1210-1217.
7. H. Engström, The first-order vestibular neuron, Fourth Symposium on the Role of the Vestibular Organs in Space Exploration, vol. SP-187, US Govt. Printing Office, Washington, DC, 1970, pp. 123-134.
8. Eybalin M. Neurotransmitters and neuromodulators of the mammalian cochlea. Physiol. Rev. 73:1993;309-373.
9. Fujita S., Usami S., Shinkawa H., Sato K., Kiyama H., Tohyama M. Expression of NMDA receptor subunit mRNA in the vestibular ganglion of the rat and guinea-pig. NeuroReport. 5:1994;862-864.
10. Goldberg J.M., Lysakowski A., Fernandez C. Morphological and ultrastructural studies in the mammalian cristae ampullares. Hearing Res. 49:1990;89-102.
11. Goldberg J.M. Theoretical analysis of intercellular communication between the vestibular type I hair cell and its calyx ending. J. Neurophysiol. 76:1996;1942-1957.
12. Hjelle O.P., Chaudhry F.A., Ottersen O.P. Antisera to glutathione: characterization and immunocytochemical application to the rat cerebellum. Eur. J. Neurosci. 6:1994;793-804.
13. Kataoka Y., Ohmori H. Activation of glutamate receptors in response to membrane depolarization of hair cells isolated from chick cochlea. J. Physiol. 477:1994;403-414.
14. Matsubara A., Laake J.H., Davanger S., Usami S., Ottersen O.P. Organization of AMPA receptor subunits at a glutamate synapse: a quantitative immunogold analysis of hair cell synapses in the inner ear. J. Neurosci. 16:1996;4457-4467.
15. Niedzielski A.S., Wenthold R.J. Expression of AMPA, kainate, and NMDA receptor subunits in cochlear and vestibular ganglia. J. Neurosci. 15:1995;2338-2353.
16. Ottersen O.P., Takumi Y., Matsubara A., Landsend A.S., Laake J.H., Usami S. Molecular organization of a type of peripheral glutamate synapse: the afferent synapses of hair cells in the inner ear. Prog. Neurobiol. 54:1998;127-148.
17. Petralia R.S., Wenthold R.J. Light and electron immunocytochemical localization of AMPA-selective glutamate receptors in the rat brain. J. Comp. Neurol. 318:1992;329-354.
18. Raymond J., Demêmes D., Nieoullon A. Neurotransmitters in vestibular pathways. Prog. Brain Res. 76:1988;29-43.
19. Ross M.D. Anatomic evidence for peripheral neural processing in mammalian graviceptors. Aviat. Space Environ. Med. 56:1985;338-343.
20. Usami S., Matsubara A., Fujita S., Shinkawa H., Hayashi M. NMDA (NMDAR1) and AMPA-type (GluR2/3) receptor subunits are expressed in the inner ear. NeuroReport. 6:1995;1161-1164.
21. Usami S., Ottersen O.P. Differential cellular distribution of glutamate and glutamine in the rat vestibular end organs: an immunocytochemical study. Brain Res. 676:1995;285-292.
22. Usami S., Osen K.K., Zhang N., Ottersen O.P. Distribution of glutamate-like and glutamine-like immunoreactivities in the rat organ of Corti: a light microscopic and semiquantitative electron microscopic analysis with a note on the localization of aspartate. Exp. Brain Res. 91:1992;1-11.
23. van Lookeren Campagne M., Oestreicher B., van der Krift T.P., Gispen W.H., Verkleij A.J. Freeze-substitution and Lowicryl HM20 embedding of fixed rat brain: suitability for immunogold ultrastructural localization of neural antigens. J. Histochem. Cytochem. 39:1991;1267-1279.
24. Wenthold R.J., Yokotani N., Doi K., Wada K. Immunochemical characterization of the non-NMDA glutamate receptor using subunit-specific antibodies. Evidence for a hetero-oligomeric structure in rat brain. J. Biol. Chem. 267:1992;501-507.
25. Wersäll J. Studies on the structure and innervation of the sensory epithelium of the cristae ampullares in the guinea pig. A light and electron microscopic investigation. Acta Oto-Laryngol. Suppl. (Stockh.). 126:1956;1-85.
26. Yamashita M., Ohmori H. Synaptic responses to mechanical stimulation in calyceal and bouton type vestibular afferents studied in an isolated preparation of semicircular ampullae of chicken. Exp. Brain Res. 80:1990;475-488.