A simple, two-component buffer enhances use of chromatofocusing for processing of therapeutic proteins

Biotechnology and Bioengineering

Volumn 62, Issue 2, 1999, Pages 208-215

  Logan, Kathleen A ^a   Lagerlund, Inger ^b   Chamow, Steven M ^c,d  

^a NOTRE DAME DE NAMUR UNIVERSITY   (United States)

^b GE Healthcare Bio Sciences AB   (Sweden)

^c GENENTECH INC   (United States)

^d SCIOS INC   (United States)

Author keywords

Biochemical manufacturing; Chromatofocusing; Multicomponent buffers; PH gradients; Preparative chromatography; Therapeutic proteins

Indexed keywords

BIOCHEMISTRY; CHEMICAL REACTORS; INDUSTRIAL APPLICATIONS; ION EXCHANGE; ION EXCHANGERS; PH EFFECTS; PROTEINS;

CATIONIC BUFFER COMPONENTS; CHROMATOFOCUSING; PH GRADIENTS; SEPHAROSE FAST FLOW ION EXCHANGERS; THERAPEUTIC PROTEINS;

CHROMATOGRAPHY;

2 DIETHYLAMINOETHANOL; BUFFER; CARBONATE DEHYDRATASE; CD4 ANTIGEN; HUMAN GROWTH HORMONE; IMMUNOGLOBULIN G; MYOGLOBIN; PROTEIN; RIBONUCLEASE A; SEPHAROSE;

ARTICLE; CHROMATOFOCUSING; PH; PROTEIN PROCESSING;

BIOTECHNOLOGY; BUFFERS; CHROMATOGRAPHY, ION EXCHANGE; ESCHERICHIA COLI; EVALUATION STUDIES; HUMAN GROWTH HORMONE; HUMANS; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 0033585490     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990120)62:2<208::AID-BIT11>3.0.CO;2-8     Document Type: Article

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