Feedback connections to the lateral geniculate nucleus and cortical response properties

Science

Volumn 286, Issue 5444, 1999, Pages 1552-1554

  Murphy, Penelope C ^a,b   Duckett, Simon G ^a   Sillito, Adam M ^b  

^a ST GEORGE'S UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BRAIN CELL; BRAIN CORTEX; FEEDBACK SYSTEM; HUMAN; HUMAN CELL; LATERAL GENICULATE BODY; PRIORITY JOURNAL; SYNAPSE; THALAMUS; VISUAL SYSTEM;

ANIMALS; AXONS; BRAIN MAPPING; CATS; FEEDBACK; GENICULATE BODIES; LYSINE; PRESYNAPTIC TERMINALS; VISUAL CORTEX; VISUAL PATHWAYS;

GOES;

EID: 0033585037     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5444.1552     Document Type: Article

References (31)

1. J. R. Wilson, M. J. Friedlander, S. M. Sherman, Proc. R. Soc. London Ser. B Biol. Sci. 221, 411 (1984).
2. V. M. Montero, Exp. Brain Res. 86, 257 (1991).
3. A. Erisir, S. C. Van Horn, S. M. Sherman, Proc. Natl. Acad. Sci. U.S.A. 94, 1517 (1997).
4. F. C. Murphy and A. M. Sillito, J. Neurosci. 16, 1180 (1996).
5. _, J. Physiol. (London) 494, P17 (1996).
6. A. R. Harvey, J. Physiol. (London) 302, 507 (1980).
7. K. L. Grieve and A. M. Sillito, J. Neurosci. 15, 4868 (1995).
8. A. M. Sillito, J. Cudeiro, P. C. Murphy, Exp. Brain Res. 93, 6 (1993).
9. F. J. Varela and W. Singer, Exp. Brain Res. 66, 10 (1987).
10. J. Cudeiro and A. M. Sillito, J. Physiol. (London) 490, 481 (1996).
11. G. H. Henry, A. Michalski, B. M. Wimborne, R. J. McCart, Prog. Neurobiol. (Oxford) 43, 381 (1994).
12. A. M. Sillito, H. E. Jones, G. L. Gerstein, D. C. West, Nature 369, 479 (1994).
13. -1 Flaxedil) cats that were prepared for visual physiology as described in (4), in accordance with government guidelines regarding the care and welfare of experimental animals. Cells in layer VI of the visual cortex were recorded with micropipettes filled with 2 to 4% biocytin in trisbuffered 1 M KCl, pH 7.2, (Sigma) and labeled either intracellularly (by pressure ejection for 10 min at 25 psi, 70 to 100 megohms impedance) or extracellularly (with 180 to 260 nA positive current for 1 s on, 1 s off for 50 to 80 min with a 1 μm diameter tip). Animals were perfused after 18 to 40 hours.
14. D. Pinault, J. Neurosci. Methods 65, 113 (1996).
15. K. Albus, Exp. Brain Res. 24, 181 (1975).
16. P. E. Maldonado and C. M. Gray, Vis. Neurosci. 13, 509 (1996).
17. Dorsal LGN was stereotaxically blocked to give a sectioning plane precisely aligned along the mediolateral axis and tilted 20° rostral to caudal so as to lie parallel to the visual projection columns. Sections were treated for biocytin histochemistry. Fully stained axons were identified and drawn with a camera lucida and then reconstructed from serial sections. For each section, the A layers were split into three equal sublayers and subdivided into 90-μm-wide columns oriented perpendicular to the laminar borders. Adjacent sections were accurately aligned, with the reconstructed axons and boundaries as a guide, to give a continuous three-dimensional array of compartments. After correction for tissue shrinkage, these compartments measured 90 μm mediolateral by 55 μm rostrocaudal (that is, the section thickness) by one-third the depth of each A layer. The number of synaptic boutons in each compartment was counted before the array was collapsed by summing those compartments relating to retinotopically corresponding points. In the subsequent analysis, boutons from both A layers were included for binocular axons, and only those from the dominant layer (4) were included for monocular axons.
18. K. S. Sanderson, J. Comp. Neurol. 143, 101 (1971).
19. W. M. Usrey and R. C. Reid, Annu. Rev. Physiology 61, 435 (1999).
20. L. C. Katz, J. Neurosci. 7, 1223 (1987).
21. B. Ahmed, J. C. Anderson, R. J. Douglas, K. A. C. Martin, J. C. Nelson, J. Comp. Neurol. 341, 39 (1994).
22. A. M. Sillito, K. L. Grieve, H. E. Jones, J. Cudeiro, J. Davis, Nature 378, 492 (1995).
23. A. Das and C. D. Gilbert, Nature 399, 655 (1999).
24. I. M. Andolina, W. Wang, H. E. Jones, G. L. Gerstein, A. M. Sillito, unpublished data.
25. W. Singer and C. M. Gray, Annu. Rev. Neurosci. 18, 555 (1995).
26. P. C. Murphy and A. M. Sillito, Nature 329, 727 (1987).
27. D. A. McCormick and M. Von Krosigk, Proc. Natl. Acad. Sci. U.S.A. 89, 2774 (1992).
28. C. Koch, Neuroscience 23, 399 (1987).
29. D. H. Hubel and T. N. Wiesel, J. Physiol. (London) 160, 106 (1962).
30. O. L. Davies and P. L. Goldsmith, Eds., Statistical Methods in Research and Production (Oliver and Boyd, Edinburgh, 1984), p. 208.
31. Many thanks to S. Claxton for excellent technical assistance and to J. M. Bland for invaluable help with the statistical analysis. Supported primarily by Medical Research Council program grant number G8519936, with additional funding from the Physiological Society and St George's Hospital Medical School.