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Volumn 9, Issue 8, 1999, Pages 1115-1120

Complexation study and anticellular activity enhancement by doxorubicin- cyclodextrin complexes on a multidrug-resistant adenocarcinoma cell line

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRACYCLINE ANTIBIOTIC AGENT; CYCLODEXTRIN; DOXORUBICIN; ISOPROTEIN;

EID: 0033583495     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(99)00150-X     Document Type: Article
Times cited : (36)

References (20)
  • 12
    • 0013533666 scopus 로고    scopus 로고
    • note
    • The Doxorubicin was purchased from Pharmacia (Saclay, France); and the cyclodextrins from Wacker-Chemie GmBH (Lyon, France). The products were solubilised in distilled water and sterilised by filtration through an 0.22 μm membrane (Millipore, Molsheim, France) before use. The human rectal adenocarcinoma cell line HRT-18 was supplied by Laporte (INRA,Jouy-en-Josas, France) and was grown in RPMI 1640 medium (ICN Biomedicals Inc. Costa Mesa, CA, USA) supplemented with fetal calf serum (Life Biotechnologies, Eragny, France) and Ofloxacin (Roussel Uclaf, Romainville, France). The cells were passaged using Trypsin-EDTA (Difco, Michigan, USA). The cellular viability was evaluated with MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, Sigma, St-Louis, MO, USA).
  • 13
    • 0013531536 scopus 로고    scopus 로고
    • note
    • -4 M; 2mL) was gradually added to varying concentrations of α, β or γ-Cd solutions. The final solutions (10 mL) were equilibrated overnight under stirring in the dark at r.t.. Finally, solutions were lyophilised and the resulting powders were stored under argon and in the dark at -20°C.
  • 14
    • 0013550964 scopus 로고    scopus 로고
    • Fluorescence spectra have been recorded on a Fluorolog II (Spex-Jobin-Yvon) photon counting spectrometer, CD spectra on a CD6 (Jobin Yvon) spectrometer
    • Fluorescence spectra have been recorded on a Fluorolog II (Spex-Jobin-Yvon) photon counting spectrometer, CD spectra on a CD6 (Jobin Yvon) spectrometer.
  • 15
    • 0013523022 scopus 로고    scopus 로고
    • note
    • 20 Absorbances in viability assays were measured on a Titertek Multiscan MCC/340 MKII (Labsystems, Helsinki, Finland) at 540 nm on cell culture 96 well microplates. Results shown are average values from triplicate experiments.
  • 16
    • 0013533667 scopus 로고    scopus 로고
    • note
    • 2 (5%) for different post-inoculation times without any change of medium. Each assay was tested in triplicate. Three blank wells received only the culture medium and three wells of cells without drugs serve as controls. After incubation, the medium was removed, the cells washed with saline phosphate buffer (PBS, pH = 7.3, 37°C) and PBS (300 μL) containing SDS (1%) was added for 15 min at 37°C. The cell suspension was harvested with cold PBS (3mL). The amount of DX was quantified by spectrofluorimetry. Each experiment was done in triplicate, with 10% standard deviation average.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.