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0013533666
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note
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The Doxorubicin was purchased from Pharmacia (Saclay, France); and the cyclodextrins from Wacker-Chemie GmBH (Lyon, France). The products were solubilised in distilled water and sterilised by filtration through an 0.22 μm membrane (Millipore, Molsheim, France) before use. The human rectal adenocarcinoma cell line HRT-18 was supplied by Laporte (INRA,Jouy-en-Josas, France) and was grown in RPMI 1640 medium (ICN Biomedicals Inc. Costa Mesa, CA, USA) supplemented with fetal calf serum (Life Biotechnologies, Eragny, France) and Ofloxacin (Roussel Uclaf, Romainville, France). The cells were passaged using Trypsin-EDTA (Difco, Michigan, USA). The cellular viability was evaluated with MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide, Sigma, St-Louis, MO, USA).
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13
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0013531536
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note
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-4 M; 2mL) was gradually added to varying concentrations of α, β or γ-Cd solutions. The final solutions (10 mL) were equilibrated overnight under stirring in the dark at r.t.. Finally, solutions were lyophilised and the resulting powders were stored under argon and in the dark at -20°C.
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14
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0013550964
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Fluorescence spectra have been recorded on a Fluorolog II (Spex-Jobin-Yvon) photon counting spectrometer, CD spectra on a CD6 (Jobin Yvon) spectrometer
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Fluorescence spectra have been recorded on a Fluorolog II (Spex-Jobin-Yvon) photon counting spectrometer, CD spectra on a CD6 (Jobin Yvon) spectrometer.
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15
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0013523022
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note
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20 Absorbances in viability assays were measured on a Titertek Multiscan MCC/340 MKII (Labsystems, Helsinki, Finland) at 540 nm on cell culture 96 well microplates. Results shown are average values from triplicate experiments.
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16
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0013533667
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note
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2 (5%) for different post-inoculation times without any change of medium. Each assay was tested in triplicate. Three blank wells received only the culture medium and three wells of cells without drugs serve as controls. After incubation, the medium was removed, the cells washed with saline phosphate buffer (PBS, pH = 7.3, 37°C) and PBS (300 μL) containing SDS (1%) was added for 15 min at 37°C. The cell suspension was harvested with cold PBS (3mL). The amount of DX was quantified by spectrofluorimetry. Each experiment was done in triplicate, with 10% standard deviation average.
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17
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0013558412
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Duchêne D., Ed.; Edition de Santé, Paris
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Minutes
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Lechat, F.2
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18
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0025633165
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Beckers, O.; Beijnen, J.H.; Otagiri, M.; Bult, A.; Underberg, W.J.M. Pharm. Biomed. Anal. 1990, 8, 671.
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0000817098
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Reilley, C.N., Ed. Interscience Publishers, John Wiley & Sons
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Higuchi, T.; Connors, K.A. In Advances in Analytical Chemistry and Instrumentation, Reilley, C.N., Ed. Interscience Publishers, John Wiley & Sons, 1965; Vol. 4, pp 117-212.
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Higuchi, T.1
Connors, K.A.2
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0028673204
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