Water vapor absorption in arthropods by accumulation of myoinositol and glucose

Science

Volumn 285, Issue 5435, 1999, Pages 1909-1911

  Bayley, Mark ^a   Holmstrup, Martin ^a  

^a NATIONAL ENVIRONMENTAL RESEARCH INSTITUTE   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCOSE; INOSITOL; WATER;

ADAPTATION; ANIMAL EXPERIMENT; ARTHROPOD; ARTICLE; BODY WATER; CONTROLLED STUDY; DEHYDRATION; DESICCATION; GLUCOSE METABOLISM; HUMIDITY; NONHUMAN; OSMOTIC PRESSURE; PRIORITY JOURNAL; SOIL; WATER ABSORPTION; WATER VAPOR;

ANIMALIA; ARTHROPODA; CANDIDA; FOLSOMIA CANDIDA; INVERTEBRATA;

EID: 0033578675     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5435.1909     Document Type: Article

References (24)

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18. Folsomia candida is adapted to the range of humidities it will encounter in the soil. The water vapor absorption by F. candida at 98% RH is therefore of equal ecological importance as the water uptake of a desert insect at 50% RH.
19. We did not investigate the process at lower RH, but we expect it to work at even lower humidities because increasing mortality only occurs at RH below 97%. The soil is a very buffered environment, and drying of the soil is a rather stow process. This implies that SP accumulation in F. candida will be induced well before the lethal limit is reached.
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22. -1 of water, respectively, as described (6). Each of these replicates contained 10 animals for measurement of water content and SPs and 20 for the measurement of body fluid osmotic pressure. The animals with access to free water were kept on the Petri dishes until sampling, when the appropriate numbers were randomly chosen for analyses. During the following 7 days, water content, osmotic pressure of body fluids, and the concentration of SPs were measured at the intervals shown in figures. At each sampling time, three replicates were removed for measurement of osmotic pressure and five for measurement of water content and SP concentration. Total water content was determined gravimetrically (grams per gram of dry weight) and converted to OAW by use of the relation given by (16).
23. 2O and filtered through a 0.2-μl filter (Nylon acrodisc; Gelman Sciences, Ann Arbor, MI) ready for high-performance liquid chromatography (HPLC) analysis. Samples of 25 μl were run in triplicate on a Shimadzu (Tokyo, Japan) HPLC system with a LC-6A pump, a 5IL-6B auto-injector, and a C-R4AX integrator. SPs were separated by ion exchange chromatography with a Supelcogel-Ca column (30 mm by 7.8 mm) and a Supelcogel-Ca guard column maintained at 55°C with pure water as the mobile phase. SPs were detected with an evaporative light scattering detector (Sedex 55). Concentrations were calculated from a standard curve with SP standards including trehalose, myoinositol, glucose, mannitol, and sorbitol. Recovery of internal standard averaged 75% (SD 5%). The colligative contribution of SPs to the animals' osmotic pressure was calculated with the conversion table in (17). A supplementary gas chromatography analysis showed that glycerol content was not elevated in drought-stressed animals and that no traces of erythritol, ribitol, fructose, or sucrose were found.
24. This study received financial support from the Danish Natural Science Research Council. We are indebted to M. H. Petersen for technical assistance in chemical analyses and to H. Ravn for advice concerning the HPLC analysis.