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Volumn 38, Issue 24, 1999, Pages 3654-3657

Tightening the belt on polymerases: Evaluating the physical constraints on enzyme substrate size

Author keywords

DNA recognition; Polymerases; RNA; Transcription

Indexed keywords

CIRCULAR DNA; DNA POLYMERASE; RNA POLYMERASE;

EID: 0033576703     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1521-3773(19991216)38:24<3654::AID-ANIE3654>3.0.CO;2-S     Document Type: Article
Times cited : (34)

References (24)
  • 14
    • 0343047172 scopus 로고    scopus 로고
    • PhD thesis, University of Rochester
    • S. L. Daubendiek, PhD thesis, University of Rochester, 1997.
    • (1997)
    • Daubendiek, S.L.1
  • 16
    • 0030841496 scopus 로고    scopus 로고
    • E. Alazzouzi, N. Escaja, A. Grandas, E. Pedroso, Angew. Chem. 1997, 109, 1564-1567; Angew. Chem. Int. Ed. Engl. 1997, 36, 1506-1508.
    • (1997) Angew. Chem. Int. Ed. Engl. , vol.36 , pp. 1506-1508
  • 17
    • 0343918823 scopus 로고    scopus 로고
    • There are two expected oligonucleotide products of Taq I cleavage, with length n and n + 1 (where n is the circle length). The n-length product arises from normal cleavage away from the ends of the repeating sequence; the n + 1 product arises from cleavage near the 3′ terminus of a given strand. The 3′ terminus for most strands is the C immediately after the Taq 1 site (TCGA); this is because dGTP concentration is kept low during radiolabeling, leading to termination prior to insertion of the G
    • There are two expected oligonucleotide products of Taq I cleavage, with length n and n + 1 (where n is the circle length). The n-length product arises from normal cleavage away from the ends of the repeating sequence; the n + 1 product arises from cleavage near the 3′ terminus of a given strand. The 3′ terminus for most strands is the C immediately after the Taq 1 site (TCGA); this is because dGTP concentration is kept low during radiolabeling, leading to termination prior to insertion of the G.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.