Time-resolved fluoroimmunoassay

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 13, Issue 3, 1999, Pages 215-222

  Degan, Paolo ^a   Podestà, Adriano ^b   Montagnoli, Giorgio ^b  

^a UNIVERSITY OF GENOA   (Italy)

^b UNIVERSITY OF PISA   (Italy)

Author keywords

Europium ion; Flourescence enhancement; Fluoimmunoassay; Time resolution

Indexed keywords

EUROPIUM; PENTETIC ACID;

ANALYTIC METHOD; ANTIBODY LABELING; ANTIBODY TITER; ANTIGEN ANTIBODY REACTION; ARTICLE; FLUOROIMMUNOASSAY;

EID: 0033573552     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:13:3:215     Document Type: Article

References (17)

1. 1. Soini, E. and Hemmilä, I. (1979) Fluoroimmunoassay: present status and key problems. Clin. Chem. 25, 353-362.
2. 2. Hemmilä, I., Dakubu, S., Mukkala, V.-M., Siitari, H., and Lövgren, T. (1984) Europium as a label in time-resolved immunofluorometric assays. Anal. Biochem. 137, 335-343.
3. 3. Ekins, R. P. and Dakubu, S. (1985) The development of high sensitivity pulsed light, time-resolved fluoroimmunoassays. Pure Appl. Chem. 57, 473-482.
4. 4. Mukkala, V.-M., Mikola, H., and Hemmilä, I. (1989) The synthesis and use of activated N-benzyl derivatives of diethylenetriaminetetraacetic acids: alternative reagents for labeling of antibodies with metal ions. Anal. Biochem. 178, 319-325.
5. 5. Keelan, J. A., France, J. T., and Barling, P. M. (1987) An alternative fluorescence enhancement solution for use in lanthanide-based time resolved fluoroimmunoassays. Clin. Chem. 33, 2292-2295.
6. 6. Degan, P., Abbondandolo, A., and Montagnoli, G. ( 1990) A new fluorescence enhancement solution for europium-based time-resolved fluoroimmunoassays J. Biolum. Chemilum. 5, 207-212.
7. 7. Evangelista, R. A., Pollak, A., Brian, A., Templeton, E. F., Morton, R. C., and Diamandis, E. P. (1988) A new europium chelate for protein labelling and time-resolved fluorometric applications. Clin. Biochem. 21, 173-178.
8. 8. Diamandis, E. P., Morton, R. C., Reichstein, E. and Khosravi, M. J. (1988) Multiple fluorescence labeling with europium chelators. Application to time-resolved fluoroimmunoassays. Anal. Chem. 61, 48-52.
9. 9. Diamandis, E. P. (1988) Immunoassays with time-resolved fluorescence spectroscopy: principles and applications. Clin. Biochem. 21, 139-150.
10. 10. Ferguson, R. A., Yu He, Kalyvas M., Zammit S., and Diamandis E. (1996) Ultra sensitive detection of prostate-specific antigen by a time-resolved immunofluorometric assay and the Immulite immunochemiluminescent third-generation assay: potential applications in prostate and breast cancers. Clin. Chem. 42, 675-684.
11. 11. Degan, P., Montesano, R., Abbondandolo, A., and Montagnoli, G. (1990). Time resolved fluoroimmunoassay of 7-methyl-2'-deoxyguanosine imidazole (ring open) J. Immunol. Methods 133, 119-125.
12. 12. Ekins, R. (1981) Merits and disadvantage of different labels and methods of immunoassay, in Immunoassays for the '80s (Voller, A. Bartlett, A., and Bidwell, D., eds.), MTP Press, Lancaster, pp. 5-16.
13. 13. Harris, M. I. and Coleman, J.E. (1968) The biosynthesis of Apo-and metalloalkaline phosphatase of Escherichia coli. J. Biol. Chem. 243, 5063-5073.
14. 14. Krejcarek, G. E. and Tucker, K. L. (1977) Covalent attachment of chelating group to macromolecules. Biochem. Biophys. Res. Commun. 77, 581-585.
15. 15. Ius, A., Ferrara, L., Meroni, G., and Bacigalupo, M. (1985) Eu-conjugated protein-A as a label of IGg antibodies Fresenius Z. Anal. Chem. 322, 509-510.
16. 16. Hemmilä, I., Holttinen, S., Pettersson, K., and Lövgren, T. (1987) Double-label time-resolved immunofluorometry of lutropin and follitropin in serum. Clin. Chem. 33, 2281-2283.
17. 17. Bador, R., Déchaud, H., Claustrat, F., and Desuzinges, C. (1987) Europium and samarium as labels in time-resolved immunofluorometric assay of follitropin. Clin. Chem. 33, 48-51.