A fluorescence-based high-performance liquid chromatographic assay to determine acid ceramidase activity

Analytical Biochemistry

Volumn 274, Issue 2, 1999, Pages 264-269

  He, Xingxuan ^a   Li, Chi Ming ^a   Park, Jae Ho ^a   Dagan, Arie ^b   Gatt, Shimon ^b   Schuchman, Edward H ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Acid ceramidase; Farber disease; Fluorescent ceramide; High performance liquid chromatography

Indexed keywords

ALKANOLAMINES; CELL CULTURE; CERAMIDES; DIGITAL STORAGE; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUIDS; RADIOACTIVITY;

ACID CERAMIDASE; CHROMATOGRAPHIC TECHNIQUES; EXPERIMENTAL CONDITIONS; FEMTOMOLE QUANTITIES; FLUORESCENT CERAMIDE; LIQUID CHROMATOGRAPHIC ASSAY; LYSOSOMAL ENZYMES; RADIOACTIVE SUBSTRATES;

FATTY ACIDS;

ACYLSPHINGOSINE DEACYLASE; CERAMIDE; LAURIC ACID; LYSOSOME ENZYME;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; FARBER DISEASE; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; KIDNEY; LYMPHOBLAST; MOUSE; NONHUMAN; PRIORITY JOURNAL; SKIN FIBROBLAST;

ANIMALIA;

EID: 0033570079     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4284     Document Type: Article

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