Biosafety considerations for flow cytometric analysis of human immunodeficiency virus-infected samples

Communications in Clinical Cytometry

Volumn 38, Issue 5, 1999, Pages 195-200

  Schmid, Ingrid ^a,d   Kunkl, Annalisa ^b   Nicholson, Janet K A ^c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF GENOA   (Italy)

^c CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^d 12 236 Factor Building ^*  (United States)

Author keywords

Biohazard; Flow cytometry; HIV; Laboratory safety; Occupational health

Indexed keywords

2 PROPANOL; ALCOHOL; CHLORINE; DISINFECTANT AGENT; FORMALDEHYDE; HYPOCHLORITE;

AEROSOL; ARTICLE; CELL SELECTION; CHEMOPROPHYLAXIS; CLINICAL LABORATORY; DISINFECTION; FLOW CYTOMETRY; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 2; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; INFECTION RISK; INSTRUMENTATION; LABORATORY AUTOMATION; LABORATORY PERSONNEL; LYMPHOCYTE COUNT; NEEDLESTICK INJURY; OCCUPATIONAL HAZARD; OCCUPATIONAL SAFETY; PRACTICE GUIDELINE; PRIORITY JOURNAL; SKIN ABRASION; STAFF TRAINING; TISSUE FIXATION; TRANSPORT MEDIUM; ULTRAVIOLET IRRADIATION; VIRUS INACTIVATION; VIRUS INFECTIVITY; VIRUS TRANSMISSION; WASTE DISPOSAL;

CELL SEPARATION; CONTAINMENT OF BIOHAZARDS; FLOW CYTOMETRY; HIV INFECTIONS; HUMANS; LABORATORIES, HOSPITAL; OCCUPATIONAL HEALTH; SAFETY MANAGEMENT; SPECIMEN HANDLING;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 2;

EID: 0033569623     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19991015)38:5<195::AID-CYTO1>3.0.CO;2-P     Document Type: Article

References (37)

1. Aloisio CH, Nicholson JKA. Recovery of infectious human immunodeficiency virus from cells treated with 1% paraformaldehyde. J Immunol Methods 1990;128:281-285.
2. Ammann AJ, Abrams D, Conant M, Chudwin D, Cowan M, Volberding P, Lewis B, Casavant C. Acquired immune dysfunction in homosexual men: immunologic profiles. Clin Immunol Immunopathol 1983;27: 315-325.
3. Centers for Disease Control. Recommendations for prevention of HIV transmission in health-care settings. Morb Mortal Wkly Rep 1987;36 (suppl no. 2S):3S-18S.
4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. Morb Mortal Wkly Rep 1988;37:378-388.
5. Centers for Disease Control. Biosafety in microbiological and biomedical laboratories. Washington: U.S. Government Printing Office.; 1993.
6. Centers for Disease Control. Update: provisional public health service recommendations for chemoprophylaxis after occupational exposure to HIV. Morb Mortal Wkly Rep 1996;45:468-472.
7. CoryJM, Rapp F, Ohlsson-Wilhelm BM. Effects of cellular fixatives on human immunodeficiency virus production. Cytometry 1990;11:647-651.
8. Druce JD, Jardine D, Locarnini SA, Birch CJ. Susceptibility of HIV to inactivation by disinfectants and ultraviolet light. J Hosp Inf 1995;152: 400-403.
9. Ericson JG, Trevino AV, Toedter GP, Mathers LE, Newbound GC, Lairmore MD. Effects of whole blood lysis and fixation on the infectivity of human T-lymphotrophic virus type 1(HTLV-1). Cytometry 1994;18:49-54.
10. Ferbas J, Chadwick KR, Logar A, Patterson AE, Gilpin RW, Margolick JB. Assessment of aerosol containment on the ELITE flow cytometer. Cytometry 1995;22:45-47.
11. Giorgi JV. Cell sorting of biohazardous specimens for assay of immune function. Methods Cell Biol 1994;42:359-369.
12. Giorgi JV, LandayA: HIV infection: diagnosis and disease progression evaluation. In: Darzynkiewicz Z, Robinson JP, Crissman HA, editors. Flow cytometry. 2nd ed. Orlando, FL: Academic Press; 1994. p 437-455.
13. + T cell levels: results of 6 years of follow-up. J Acquir Immune Defic Syndr 1993;6:904-912.
14. Herbein G, Illei P, Montaner LJ, William J, Gordon S. Comparison of p24 measurement by ELISA versus indicator cells for detecting residual HIV infectivity in vitro. J Virol Methods 1996;58:167-173.
15. Landay A, Ohlsson-Wilhelm BM, Giorgi JV. Application of flow cytometry to the study of HIV infection. AIDS 1990;4:479-497.
16. Laser Institute of America. Laser Safety Guide. Orlando: Laser Institute of America; 1993.
17. Lifson JD, Sasaki DT, Engleman EG. Utility of formaldehyde fixation for flow cytometry and inactivation of the AIDS associated retrovirus. J Immunol Methods 1986;86:143-149.
18. + T cells is a marker of poor prognosis in HIV infection: results of 6 years of follow-up. Cytometry 1996;26:1-7.
19. Martin LS, Loskoski SL, McDougal JS. Inactivation of human T-lymphotropic virus type III/lymphadenopathy-associated virus by formaldehyde reagents. Appl Environ Microbiol 1987;53:708-709.
20. Martin LS, McDougal JS, Loskoski SL. Disinfection and inactivation of the human T lymphotropic virus type III/ lymphadenopathy-associated virus. J Infect Dis 1985;152:400-403.
21. Merrill JT. Evaluation of selected aerosol-control measures on flow sorters. Cytometry 1981;1:342-345.
22. National Committee for Clinical Laboratory Standards. Procedures for the handling and processing of blood specimens. Document H18-A. 1990.
23. National Committee for Clinical Laboratory Standards. Clinical laboratory waste management. Document GP5-A. 1993.
24. National Committee for Clinical Laboratory Standards. Protection of laboratory workers from infectious disease transmitted by blood, body fluids, and tissue. Document M29-A. 1997.
25. Nicholson JKA, Browning SW. Ability of "Optilyse" lysing/fixing reagents to inactivate HIV-infected H9 cells in whole blood. J Immunol Methods 1994;168:283-284.
26. Nicholson JKA, Browning SW, Orloff SL, McDougal JS. Inactivation of HIV-infected H9 cells in whole blood preparations by lysing/fixing reagents used in flow cytometry. J Immunol Methods 1993;160:215-218.
27. Resnick L, Veren K, Salahuddin SZ, Tondreau S, Markham PD. Stability and inactivation of HTLV-III/LAV under clinical and laboratory environments. J Am Med Assoc 1986;255:1887-1891.
28. Sattar SA, Springthorpe VS. Survival and disinfectant inactivation of the human immunodeficiency virus: a critical review. Rev Infect Dis 1991;13:430-447.
29. Schmid I, Hultin LE, Ferbas J. Testing the efficiency of aerosol containment during cell sorting. In: Robinson JP, Darzynkiewicz Z, Dean PN, Dressler LG, Tanke HJ, Rabinovitch PS, Stewart CC, Wheeless LL, editors. Current protocols in cytometry. New York: John Wiley & Sons; 1997. p. Unit 33.
30. Schmid I, Nicholson JKA, Giorgi JV, Janossy G, Kunkl A, Lopez PA, Perfetto S, Seamer LC, Dean PN. Biosafety guidelines for sorting of unfixed cells. Cytometry 1997;28:99-117.
31. Schroff RW, Gottlieb MS, Prince HE, Chai LL, Fahey JL. Immunological studies of homosexual men with immunodeficiency and Kaposi's Sarcoma. Clin Immunol Immunopathol 1983;27:300-314.
32. Taylor JM, Fahey JL, Detels R, Giorgi JV. CD4 percentage, CD4 number, and CD4:CD8 ratio in HIV infection: which to choose and how to use. J Acquir Immune Defic Syndr 1989;2:114-124.
33. United States Federal Code Regulation: Occupational exposure to bloodborne pathogens. CFR PART 1910.1030: 1991.
34. United States Federal Code Regulation: Hazardous materials regulations. 49 CFR Part 171-178: 1998.
35. Van Bueren J, Larkin DP, Simpson RA. Inactivation of human immunodeficiency virus type 1 by alcohols. J Hosp Inf 1994;28:137-148.
36. Van Bueren J, Simpson RA, Salman H, Farrelly HD, Cookson BD. Inactivation of HIV-1 by chemical disinfectants: sodium hypochlorite. Epidemiol Infect 1995;115:567-579.
37. World Health Organization. Guidelines for the safe transport of infectious substances and diagnostic specimens. Geneva: 1997.