Extensive clear zone and defective ruffled border formation in osteoclasts of osteopetrotic (ia/ia) rats: Implications for secretory function

Experimental Cell Research

Volumn 251, Issue 2, 1999, Pages 477-491

  Reinholt, Finn P ^a,b   Hultenby, Kjell ^a   Heinegård, Dick ^c   Marks Jr , Sandy C ^d   Norgård, Maria ^a   Anderson, Göran ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^b UNIVERSITY OF OSLO   (Norway)

^c LUND UNIVERSITY   (Sweden)

^d UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Bone matrix; Integrin subunits; Osteopetrosis; Osteopontin; Ultrastructural immunocytochemistry

Indexed keywords

ACID PHOSPHATASE; INTEGRIN; MESSENGER RNA; OSTEOPONTIN; PROTEIN KINASE P60; RNA;

ALBERS SCHOENBERG DISEASE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; BONE MATRIX; CELL ULTRASTRUCTURE; CONTROLLED STUDY; CYTOPLASM VESICLE; DNA SEQUENCE; ENZYME ACTIVITY; IMMUNOCYTOCHEMISTRY; NONHUMAN; NUCLEOTIDE SEQUENCE; ONCOGENE SRC; OSTEOCLAST; OSTEOLYSIS; PRIORITY JOURNAL; RAT; SECRETORY CELL;

ANIMALIA;

EID: 0033568206     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.1999.4585     Document Type: Article

References (59)

1. Baron R., Neff L., Louvard D., Courtory P. J. Cell-mediated extracellular acidification and bone resorption: Evidence for a low pH in resorption lacunae and localization of 100 kDa lysosomal membrane protein at the osteoclast ruffled border. J. Cell Biol. 101:1985;2210-2222.
2. Flores M., Norgård M., Heinegård D., Reinholt F. P., Andersson G. RGD-directed attachment of isolated rat osteoclasts to osteopontin, bone sialoprotein and fibronectin. Exp. Cell Res. 201:1992;526-530.
3. Reinholt F. P., Hultenby K., Oldberg Å., Heinegård D. Osteopontin - A possible anchor of osteoclast to bone. Proc. Natl. Acad. Sci. USA. 87:1990;4473-4475.
4. 3 integrin is colocalizied with vinculin and talin in podosomes of osteoclastoma giant cells. Exp. Cell Res. 182:1989;45-52.
5. Grano M., Zigrino P., Colucci S., Zambonin G., Trusolini L., Serra M., Baldini N., Teti A., Marchisio P. C., Zambonin-Zallone A. Adhesion properties and integrin expression of cultured human osteoclast-like cells. Exp. Cell Res. 212:1994;209-218.
6. Ruoslahti E. RGD and other recognition sequences for integrins. Annu. Rev. Cell Dev. Biol. 12:1996;697-715.
7. Helfrich M. H., Nesbitt S. A., Dorey E. L., Horton M. A. Integrins on rat osteoclasts: Characterization of two monoclonal antibodies (F4 and F11) to rat β3. J. Bone Miner. Res. 7:1992;345-351.
8. 3 integrin subunits in rat osteoclasts in situ. J. Bone Miner. Res. 8:1993;403-414.
9. Clover J., Dodds R. A., Gowen M. Integrin subunit expression by human osteoblasts and osteoclasts in situ and in culture. J. Cell Sci. 103:1992;267-271.
10. Hughes D. E., Salter D. M., Dedhar S., Simpson R. Integrin expression in human bone. J. Bone Miner. Res. 8:1993;527-533.
11. Hultenby K., Reinholt F. P., Heinegård D. Distribution of integrin subunits on rat methaphyseal osteoclasts and osteoblasts. Eur. J. Cell Biol. 62:1993;86-93.
12. Oldberg Å., Franzén A., Heinegård D. Cloning and sequence analysis of rat bone sialoprotein (osteopontin) cDNA reveals an arg-gly-asp cell-binding sequence. Proc. Natl. Acad. Sci. USA. 83:1986;8819-8823.
13. Franzén A., Heinegård D. Isolation and characterization of two sialoproteins present only in bone calcified matrix. Biochem. J. 232:1985;715-724.
14. Lakkakorpi P. T., Horton M. A., Helfrich M. H., Karhukorpi E. K., Väänänen H. K. Vitronectin receptor has a role in bone resorption but does not mediate tight sealing zone attachment of osteoclasts to the bone surface. J. Cell Biol. 115:1991;1179-1186.
15. Holt I., Marchall M. J. Integrin subunit β3 plays a crucial role in the movement of osteoclasts from the periosteum to the bone surface. J. Cell. Physiol. 175:1998;1-9.
16. Nakamura I., Gailit J., Sasaki T. Osteoclast integrin is present in the clear zone and contributes to cellular polarization. Cell Tissue Res. 286:1996;507-515.
17. Mark M. P., Prince C. W., Oosawa T., Gay S., Bronckers A. L. J. J., Butler W. Immunohistochemical demonstration of a 44-KD phosphoprotein in developing rat bones. J. Histochem. Cytochem. 35:1987;707-715.
18. 2-HS-glycoprotein) proteins in rat bone. J. Bone Miner. Res. 8:1993;485-496.
19. Dodds R. A., Connor J. R., James I. E., Rykaczewski E. L., Appelbaum E., Dul E., Gowen E. Human osteoclasts, not osteoblasts, deposit osteopontin onto resorption surfaces: An in vitro and ex vitro study of remodeling bone. J. Bone Min. Res. 10:1995;1666-1680.
20. Tezuka K., Sato T., Kamioka H., Nijweide P. J., Tanaka K., Matsuo T., Ohta M., Kurihara N., Hekda Y., Kumegawa M. Identification of osteopontin in isolated rabbit osteoclasts. Biochem. Biophys. Res. Commun. 186:1992;911-917.
21. Denhardt D. T., Guo X. Osteopontin: A protein with diverse functions. FASEB J. 7:1993;1475-1482.
22. Weinman J. P., Sicher H. Bone and Bones: Fundamentals of Bone Biology. 1947;Mosby, St. Louis.
23. Greep R. O. A hereditary absence of the incisor teeth. J. Heredity. 32:1941;397-398.
24. Marks S. C. Jr. Osteopetrosis in ia rat cured by spleen cells from a normal littermate. Am. J. Anat. 146:1976;331-338.
25. Marks S. C. Jr. Pathogenesis of osteopetrosis in the ia rat: Reduced bone resorption due to reduced osteoclast function. Am. J. Anat. 138:1973;165-190.
26. Ek-Rylander B., Marks S. C. Jr., Hammarström L. E., Andersson G. N. Osteoclastic acid ATPase: Biochemical and histochemical studies of osteopetrotic mutations in the rat. Bone Miner. 5:1989;309-321.
27. Soriano P., Montgomery C., Geske R., Bradley A. Targeted disruption of c-src proto-oncogene leads to osteopetrosis in mice. Cell. 64:1991;693-702.
28. c-src expression for osteoclasts to form ruffled borders and resorb bone in mice. J. Clin. Invest. 90:1992;1622-1627.
29. Horne W. C., Neff L., Chatterjee D., Lomri A., Levy J. B., Baron R. Osteoclasts express high levels of pp60c-src in association with intracellular membranes. J. Cell Biol. 119:1992;1003-1013.
30. c-src, preferentially on ruffled border membranes. FEBS Lett. 313:1992;85-89.
31. Ek-Rylander B., Barkhem T., Ljusberg J., Öhman L., Andersson K., Andersson G. Comparative studies of rat recombinant purple acid phosphatase and bone tartrate-resistant acic phosphatase. Biochem. J. 321:1997;305-311.
32. Hultenby K., Reinholt F. P., Oldberg Å., Heinegård D. Ultrastructural immunolocalization of osteopontin in metaphyseal and cortical bone. Matrix. 11:1991;206-213.
33. Tokuyasu K. T. A technique for ultracryotomy of cell suspension and tissue. J. Cell Biol. 57:1973;551-565.
34. Möller C., Arner P., Sonnenfeld T., Norstedt G. Quantitative comparison of insulin-like growth factor mRNA levels in human and rat tissues analysed by a solution hybridization assay. Mol. Endocrinol. 7:1991;213-222.
35. Ek-Rylander B., Bill P., Norgård M., Nilsson S., Andersson G. Cloning, sequence and developmental expression of a type 5, tartrate-resistant acid phosphatase of rat bone. J. Biol. Chem. 266:1991;24684-24689.
36. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:1987;156-159.
37. Fort P. H., Piechaczyk M., El Sabrouty S., Dani C. H., Jeantour P. H., Blanchard J. M. Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate dehydrogenase multigenic family. Nucl. Acids Res. 13:1985;1431-1442.
38. Zerwekh J. E., Marks S. C. Jr., McGuire J. L. Elevated serum 1,25-dihydroxyvitamin D in osteopetrotic mutations in three species. Bone Miner. 2:1987;193-199.
39. 3 subunit gene and its regulation by 1,25-dihydroxyvitamin D3. J. Biol. Chem. 268:1993;27371-27380.
40. 3. J. Biol. Chem. 268:1993;1456-1461.
41. 3-integrin subunit gene in avian osteoclast precursors. Endocrinology. 134:1994;1061-1066.
42. Arai N., Ohya K., Ogura H. Osteopontin mRNA expression during bone resorption: An in situ hybridization study of induced ectopic bone in rat. Bone Miner. 122:1993;129-145.
43. 3 potentiate bone resorption. J. Biol. Chem. 268:1993;9901-9907.
44. 3 integrin stimulates immediate cell signals in osteoclasts. J. Biol. Chem. 266:1991;20369-20374.
45. 3 integrins. Exp. Cell Res. 227:1996;40-46.
46. Helfrich M. H., Nesbitt S. A., Dorey E. L., Horton M. A. Rat osteoclasts adhere to a wide range of RGD (Arg-Gly-Asp) peptide-containing proteins, including the bone sialoproteins and fibronectine, via a β3 integrin. J. Bone Miner. Res. 7:1992;335-343.
47. Rittling S. R., Matsumoto H. N., McKee M. D., Nanci A., An X. R., Novick K. E., Kowalski A. J., Noda M., Denhardt D. T. Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro. J. Bone Miner. Res. 13:1998;1101-1111.
48. Aubin J. E. Perspectives on osteoclast adhesion and resorption: The role of podosomes. J. Bone Miner. Res. 7:1992;365-368.
49. Teti A., Marchisio P. C., Zambonin-Zallone A. Clear zone in osteoclast function: Role of podosomes in regulation of bone-resorbing activity. Am. J. Physiol. 261:1991;C1-C7.
50. Miller S. C., Marks S. C. Jr. Osteoclast kinetics in osteopetrotic (ia) rats cured by spleen cell transfers from normal littermates. Calcif. Tissue Int. 34:1982;422-427.
51. Shalhoub V., Bortell R., Jackso M. E., Marks S. C. Jr., Stein J. L., Lian J. O., Stein G. S. Transcriptionally active nuclei isolated from intact bone reflect modified levels of gene expression in skeletal development and pathology. J. Cell Biochem. 55:1994;182-189.
52. Reinholt F. P., Mengarelli-Widholm S., Ek-Rylander B., Andersson G. Ultrastructural localization of a tartrate-resistant acid ATPase in bone. J. Bone Miner. Res. 5:1990;1055-1061.
53. 3 by osteopontin stimulates phosphatidylinositol 3-hydroxyl kinase activity. Endocrinology. 136:1995;2984-2982.
54. Chackalaparampil I., Peri A., Nemir M., McKee M., Lin P. H., Mukherjee B. B., Mukherjee A. B. Cells in vivo and in vitro from osteopetrotic mice homozygous for c-src disruption show suppression of synthesis of osteopontin, a multifunctional extracellular matrix protein. Oncogene. 12:1996;1457-1467.
55. Tezuka K., Denhardt D. T., Rodan G. A., Harada S. Stimulation of mouse osteopontin promotor by v-src is mediated by CCAAT box-binding factor. J. Biol. Chem. 271:1996;22713-22717.
56. Tanaka S., Amling M., Neff L., Peyman A., Uhlman E., Levy J. B., Baron R. C-Cbl is downstream of c-Src in a signaling pathway necessary for bone resorption. Nature. 383:1996;528-531.
57. Sakai R., Iwamatsu A., Hirano N., Ogawa S., Tanaka T., Mano H., Yazaki Y., Hirai H. A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk an v-Src in a tyrosine phosphorylation-dependent manner. EMBO J. 13:1994;3748-3756.
58. Ek-Rylander B., Flores M., Wendel M., Heinegård D., Andersson G. Dephosphorylation of osteopontin and bone sialoprotein by osteoclastic tartrate-resistant acid phosphatase - Modulation of osteoclast adhesion in vitro. J. Biol. Chem. 269:1994;14853-14856.
59. Katayama Y., House C. M., Udagawa N., Kazama J. J., McFarland R. J., Martin T. J., Findlay D. M. Casein kinase 2 phosphorylation of recombinant osteopontin enhances adhesion of osteoclasts but not osteoblasts. J. Cell. Physiol. 176:1998;179-187.