Roles of Gln81 and Cys80 in catalysis by glycosylphosphatidylinositol- phospholipase C from Trypanosoma brucei

European Journal of Biochemistry

Volumn 264, Issue 3, 1999, Pages 914-920

  Rashid, Mohammad B ^a   Russell, Malissa ^a   Mensa Wilmot, Kojo ^a  

^a UNIVERSITY OF GEORGIA   (United States)

Author keywords

Glycosylphosphatidylinositol specific phospholipase C; Mutagenesis; p chloromercuriphenyl sulfonate; T. brucei

Indexed keywords

GLYCOSYLPHOSPHATIDYLINOSITOL; PHOSPHOLIPASE C;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME STRUCTURE; MUTAGENESIS; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS; TRYPANOSOMA BRUCEI;

4-CHLOROMERCURIBENZENESULFONATE; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CATALYTIC DOMAIN; CYSTEINE; DNA PRIMERS; ENZYME INHIBITORS; ESCHERICHIA COLI; GLUTAMINE; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHATIDYLINOSITOL DIACYLGLYCEROL-LYASE; PHOSPHOLIPASE C; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TRYPANOSOMA BRUCEI BRUCEI;

BACTERIA (MICROORGANISMS); FELIS CATUS; PROTOZOA; TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0033568108     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00690.x     Document Type: Article

References (26)

1. 1. Hereld, D., Krakow, J.L., Bangs, J.D., Hart, G.W. & Englund, P.T. (1986) A phospholipase C from Trypanosoma brucei which selectively cleaves the glycolipid on the variant surface glycoprotein. J. Biol. Chem. 261, 13813-13819.
2. 2. Bülow, R. & Overath, P. (1986) Purification and characterization of the membrane-form variant surface glycoprotein hydrolase of Trypanosoma brucei. J. Biol. Chem. 261, 11918-11923.
3. 3. Fox, J.A., Duszenko, M., Ferguson, M.A., Low, M.G. & Cross, G.A.M. (1986) Purification and characterization of a novel glycanphosphatidylinositol-specific phospholipase C from Trypanosoma brucei. J. Biol. Chem. 261, 15767-15771.
4. 4. Webb, H., Carnall, N., Vanhamme, L., Rolin, S., Van den Abbeele, J., Welburn, S., Pays, E. & Carrington, M. (1997) The GPI-phospholipase C of Trypanosoma brucei is nonessential but influences parasitemia in mice. J. Cell Biol. 139, 103-114.
5. 5. Mensa-Wilmot, K. & Englund, P.T. (1992) Glycosyl phosphatidylinositol-specific phospholipase C of Trypanosoma brucei: Expression in Escherichia coli. Mol. Biochem. Parasitol. 56, 311-322.
6. 6. Hereld, D., Hart, G.W. & Englund, P.T. (1988) cDNA encoding the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei. Proc. Natl Acd. Sci. USA 85, 8914-8918.
7. 7. Carrington, M., Bülow, R., Reinke, H. & Overath, P. (1989) Sequence and expression of the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei. Mol. Biochem. Parasitol. 33, 289-296.
8. 8. Morris, J.C., Lei, P.S., Zhai, H.X., Shen, T.Y. & Mensa-Wilmot, K. (1998) Inhibition of GPI phospholipase C from Trypanosoma brucei by fluoro-inositol dodecylphosphonates. Biochem. Biophys. Res. Commun. 244, 873-876.
9. 9. Morris, J.C., Ping-Sheng, L., Shen, T.Y. & Mensa-Wilmot, K. (1995) Glycan requirements of glycosylphosphatidylinositol phospholipase C from Trypanosoma brucei. Glucosaminylinositol derivatives inhibit phosphatidylinositol phospholipase C. J. Biol. Chem. 270, 2517-2524.
10. 10. Butikofer, P., Boschung, M., Brodbeck, U. & Menon, A.K. (1996) Phosphatidylinositol hydrolysis by Trypanosoma brucei glycosylphosphatidylinositol phospholipase C. J. Biol. Chem. 271, 15533-15541.
11. 11. Armah, D.A. & Mensa-Wilmot, K. (1999) S-Myristoylation of a GPI-phospholipase C in Trypanosoma brucei. J. Biol. Chem 274, 5931-5939.
12. 12. Kuppe, A., Evans, L.M., McMillen, D.A. & Griffith, O.H. (1989) Phosphatidylinositol-specific phospholipase C of Bacillus cereus: cloning, sequencing, and relationship to other phospholipases. J. Bacteriol. 171, 6077-6083.
13. 13. Daugherty, S. & Low, M.G. (1993) Cloning, expression, and mutagenesis of phosphatidylinositol-specific phospholipase C from Staphylococcus aureus: a potential staphylococcal virulence factor. Infect. Immun. 61, 5078-5089.
14. 14. Griffith, O.H., Volwerk, J.J. & Kuppe, A. (1991) Phosphatidylinositol-specific phospholipases C from Bacillus cereus and Bacillus thuringiensis. Methods Enzymol. 197, 493-502.
15. 15. Carnall, N., Webb, H. & Carrington, M. (1997) Mutagenesis study of the glycosylphosphatidylinositol phospholipase C of Trypanosoma brucei. Mol. Biochem. Parasitol. 90, 423-432.
16. 16. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
17. 17. Studier, F.W., Rosenberg, A.H., Dunn, J.J. & Dubendorf, J.W. (1990) Use of T7 RNA polymerase to direct the expression of cloned genes. Methods Enzymol. 185, 60-89.
18. 18. Mensa-Wilmot, K., Morris, J.C., Al-Qahtani, A. & Englund, P.T. (1995) Purification and use of recombinant glycosylphosphatidylinositol phospholipase C. Methods Enzymol. 250, 641-655.
19. 19. Liang, Q., Chen, L. & Fulco, A.J. (1995) An efficient and optimized PCR method with high fidelity for site-directed mutagenesis. PCR. Methods Appl. 4, 269-274.
20. 20. Mensa-Wilmot, K., Hereld, D. & Englund, P.T. (1990) Genomic organization, chromosomal localization, and developmentally regulated expression of the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei. Mol. Cell. Biol. 10, 720-726.
21. 21. Henner, D.J., Yang, M., Chen, E., Hellmiss, R., Rodriguez, H. & Low, M.G. (1988) Sequence of the Bacillus thuringiensis phosphatidylinositol-specific phospholipase C. Nucleic Acids Res. 16, 10383.
22. 22. Mensa-Wilmot, K., Doering, T.L., Raper, J., Buxbaum, L., Hart, G.W. & Englund, P.T. (1991) Glycosyl phosphatidylinositol membrane anchors in African trypanosomes. In Molecular and Immunological Aspects of Parasitism. (Wang, C.C., ed.), pp. 43-54. AAAS, Washington, DC.
23. 23. Kyte, J. & Doolittle, R.F. (1982) A simple method for displaying the hydropathic character of a protein. J. Mol.. Biol. 157, 105-132.
24. 24. Heinz, D.W., Essen, L.O. & Williams, R.L. (1998) Structural and mechanistic comparison of prokaryotic and eukaryotic phosphoinositide-specific phospholipases C. J. Mol Biol. 275, 635-650.
25. 25. Vernet, T., Tessier, D.C., Chatellier, J., Plouffe, C., Lee, T.S., Thomas, D.Y., Storer, A.C. & Menard, R. (1995) Structural and functional roles of asparagine 175 in the cysteine protease papain. J. Biol. Chem. 270, 16645-16652.
26. 26. Hendle, J., Mattevi, A., Westphal, A.H., Spee, J., de Kok, A., Teplyakov, A. & Hol, W.G. (1995) Crystallographic and enzymatic investigations on the role of Ser558, His610, and Asn614 in the catalytic mechanism of Azotobacter vinelandii dihydrolipoamide acetyltransferase (E2p). Biochemistry 34, 4287-4298.