Estrogen receptor-α gene transfer into bovine aortic endothelial cells induces eNOS gene expression and inhibits cell migration

Cardiovascular Research

Volumn 43, Issue 3, 1999, Pages 788-797

  Tan, Enqing ^a   Gurjar, Milind V ^a   Sharma, Ram V ^a   Bhalla, Ramesh C ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

Adenoviral vector; Endothelial function; Estrogen receptor; Gene expression; Gene therapy; Nitric oxide

Indexed keywords

ANTIESTROGEN; COLLAGEN TYPE 4; ESTROGEN RECEPTOR; FULVESTRANT; MESSENGER RNA; NITRIC OXIDE SYNTHASE;

ANIMAL CELL; ARTICLE; ATHEROSCLEROSIS; CARBOXY TERMINAL SEQUENCE; CATTLE; CELL FUNCTION; CELL MIGRATION; CLONING VECTOR; CONTROLLED STUDY; ENDOTHELIUM CELL; GENE EXPRESSION; GENE OVEREXPRESSION; GENE TRANSFER; GENETIC TRANSFECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RECEPTOR UPREGULATION; REGULATORY MECHANISM;

ANIMALS; AORTA; BLOTTING, NORTHERN; BRADYKININ; CATTLE; CELL MOVEMENT; CELLS, CULTURED; ENDOTHELIUM, VASCULAR; ENZYME INDUCTION; ESTRADIOL; ESTROGEN ANTAGONISTS; ESTROGEN RECEPTOR ALPHA; GENE EXPRESSION; GENE THERAPY; GENE TRANSFER TECHNIQUES; NITRIC OXIDE SYNTHASE; NITRIC OXIDE SYNTHASE TYPE III; RECEPTORS, ESTROGEN;

EID: 0033566896     PISSN: 00086363     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0008-6363(99)00159-5     Document Type: Article

References (46)

1. Mendelsohn M.E., Karas R.H. Estrogen and the blood vessel wall. Curr Opin Cardiol. 9:1994;619-626.
2. Farhat M.Y., Lavigne M.C., Ramwell P.W. The vascular protective effects of estrogen. FASEB J. 10:1996;615-624.
3. Bhalla R.C., Toth K.F., Bhatty R.A., Thompson L.P., Sharma R.V. Estrogen reduces proliferation and agonist-induced calcium increase in coronary artery smooth muscle cells. Am J Physiol. 272:1997;H1996-H2003.
4. Collins P., Shay J., Jiang C., Moss J. Nitric oxide accounts for dose-dependent estrogen-mediated coronary relaxation after acute estrogen withdrawal. Circulation. 90:1994;1964-1968.
5. Hayashi T., Yamada K., Esaki T., et al. Estrogen increases endothelial nitric oxide by a receptor-mediated system. Biochem Biophys Res Commun. 214:1995;847-855.
6. Arnal J.F., Clamens S., Pechet C., et al. Ethinylestradiol does not enhance the expression of nitric oxide synthase in bovine endothelial cells but increases the release of bioactive nitric oxide by inhibiting superoxide anion production. Proc Natl Acad Sci USA. 93:1996;4108-4113.
7. Bhalla R.C., Toth K.F., Bhatty R.A., Sharma R.V. Regulation of endothelial nitric oxide synthase in bovine aortic endothelial cells by estradiol. Stamler J.S., Gross S.S., Moncada S. The Biology of nitric oxide, Part 4. 1996;66 Portland Press, VT.
8. Harrison D.G., Sayegh H., Ohara Y., Inoue N., Venema R.C. Regulation of expression of the endothelial cell nitric oxide synthase. Clin Exp Pharmacol Physiol. 23:1996;251-255.
9. Hishikawa K., Nakaki T., Marumo T., et al. Upregulation of endothelial nitric oxide synthase by estradiol in human aortic endothelial cells. FEBS Lett. 360:1995;291-293.
10. Tschugguel W., Zhegu Z., Schneeberger C., et al. Estrogen does not induce the calcium-dependent nitric oxide syntahse in cultured human uterine endothelial and myometrial smooth muscle cells. J Vasc Res. 34:1997;281-288.
11. Macritchie A.N., Jun S.S., Chen Z., et al. Estrogen upregulates endothelial nitric oxide synthase gene expression in fetal pulmonary artery endothelium. Circ Res. 81:1997;355-362.
12. Spyridopoulos I., Sullivan A.B., Kearney M., Isner J.M., Losordo D.W. Estrogen-receptor-mediated inhibition of human endothelial cell apoptosis: estradiol as a survival factor. Circulation. 95:1997;1505-1514.
13. Venkov C.D., Rankin A.B., Vaughan D.E. Identification of authentic estrogen receptor in cultured endothelial cells: A potential mechanism for steroid hormone regulation of endothelial function. Circulation. 94:1996;727-733.
14. Morales D.E., Kelly A., McGowan B.S., et al. Estrogen promotes angiogenic activity in human umbilical vein endothelial cells in vitro and in a murine model. Circulation. 91:1995;755-763.
15. Drummond A.E., McPherson S.J., Laslett A., Hearn M.T.W. Application of a chromogenic bioassay procedure for the measurement of the proliferation of endothelial cells in vitro under the influence of the effects of steroid hormones and growth factors. J Biochem Biophys Methods. 31:1996;123-134.
16. Sankar S., Mahooti-Brooks N., Hu G., Madri J.A. Modulation of cell spreading and migration by pp125 FAK phosphorylation. J Am Pathol. 147:1995;601-608.
17. Re F., Zaneti A., Sironi M., et al. Inhibition of anchorage-dependent cell spreading triggers apoptosis in cultured human endothelial cells. J Cell Biol. 127:1994;537-546.
18. Fang S., Sharma R.V., Bhalla R.C. eNOS gene transfer inhibits PDGF-BB stimulated FAK and paxillin phosphorylation in vascular smooth muscle cells. Biochem Biophy Res Commun. 236:1997;706-711.
19. Schaller M.D., Parsons J.T. Focal adhesion kinase and associated proteins. Curr Opin Cell Biol. 6:1994;705-710.
20. Hatai M., Hashi H., Mogi A., et al. Stimulation of tyrosine- and serine-phosphorylation of focal adhesion kinase in mouse 3T3 cells by fibronectin and fibroblast growth factor. FEBS Lett. 350:1994;113-116.
21. Ekena K., Weis K.E., Katzenellenbogen J.A., Katzenellenbogen B.S. Identification of amino acids in the hormone binding domain of the human estrogen receptor important in estrogen binding. J Biol Chem. 271:1996;20053-20059.
22. Mark D.I., Karas R.H., Aronovitz M., et al. Estrogen inhibits the vascular injury response in estrogen receptor alpha-deficient mice. Nature Med. 3:1997;545-548.
23. Kim-Schulze S., McGowan K.A., Hubchak S.C., et al. Expression of an estrogen receptor by human coronary artery and umbilical vein endothelial cells. Circulation. 94:1996;1402-1407.
24. Karas R.H., Patterson B.L., Mendelsohn M.E. Human vascular smooth muscle cells contain functional estrogen receptor. Circulation. 89:1994;1943-1950.
25. Losordo D.W., Keaney M., Kim E.A., Jekanowski J., Isner J.M. Variable expression of the estrogen receptor in normal and atherosclerotic coronary arteries of premenopausal women. Circulation. 89:1994;1501-1510.
26. Lou H., Zhao Y., Delafontaine P., et al. Estrogen effects on insulin-like growth factor-I (IGF-I)-induced cell proliferation and IGF-I expression in native and allograft vessels. Circulation. 96:1997;927-933.
27. MacRitchie A.N., Jun S.S., Chen Z., et al. Estrogen upregulates endothelial nitric oxide synthase gene expression in fetal pulmonary artery endothelium. Circ Res. 81:1997;355-362.
28. Lamas S., Marsden P.A., Li G.K., Tempst P., Michel T. Endothelial nitric oxide synthase: Molecular cloning and characterization of a distinct constitutive enzyme isoform. Proc Natl Acad Sci USA. 89:1992;6348-6352.
29. Greene G.L., Gilna P., Waterfield M., et al. Sequence and expression of human estrogen receptor complementary DNA. Science. 231:1986;1150-1154.
30. Sharma R.V., Fang S., Bhalla R.C. Factors influencing adenovirus-mediated gene transfer to endothelial and vascular smooth muscle cells in vivo and in vitro. Pandalai S.G. Current development in molecular biology. 1997;41-50 Research Signpost, Trivendrum, India.
31. Shasby S.S. Endothelial cells grown on permeable membrane supports. J Tissue Cult Meth. 14:1992;247-252.
32. Sharma RV, Enqing T, Fang S, Milind VG, Bhalla RC. NOS gene transfer inhibits expression of cell cycle regulatory molecules in vascular smooth muscle cells. Am J Physiol 1999;276(Heart Circ Physiol 45):H1450-1459.
33. Sharma RV, Enqing T, Fang S, Milind VG, Bhalla RC. NOS gene transfer inhibits expression of cell cycle regulatory molecules in vascular smooth muscle cells. Am J Physiol 1999;276(Heart Circ Physiol 45):H1450-1459.
34. Sharma R.V., Bhalla R.C. PDGF-induced mitogenic signaling is not mediated through protein kinase C and c-fos pathways in VSM cells. Am J Physiol. 264:1993;C71-C79.
35. Fang S., Sharma R.V., Bhalla R.C. Enhanced recovery of injury-caused downregulation of paxillin protein by eNOS gene expression in rat carotid artery: Mechanism of NO inhibition of intimal hyperplasia? Arterioscler Thromb Vasc Biol. 19:1999;147-152.
36. 2+ and nitric oxide in bradykinin-stimulated vascular endothelial cells. Circ Res. 76:1995;922-924.
37. Karas R.H., Gauer E.A., Bieber H.E., Baur W.E., Mendelsohn M.E. Growth factor activation of the estrogen receptor in vascular cells occurs via a MAP kinase-independent pathway. J Clin Invest. 101:1998;2851-2861.
38. Hayashi T., Fukuto J.M., Ignaro L.J., Chaudhari C. Basal release of nitric oxide from aortic rings is greater in female rabbits than in male rabbits: implications for atherosclerosis. Proc Natl Acad Sci USA. 89:1992;11259-11261.
39. Bunone G., Briand P.A., Miksicek R.J., Picard D. Activation of the unliganded estrogen receptor by EGF involves the MAP kinase pathway and direct phosphorylation. EMBO J. 15:1996;2174-2183.
40. O'Malley B.W., Schrader W.T., Mani S., et al. An alternative ligand-independent pathway for activation of steroid receptors. Recent Prog Horm Res. 50:1995;333-347.
41. Huttenlocher A., Sandborg R.R., Horwitz A.F. Adhesion in cell migration. Curr Opin Cell Biol. 7:1995;697-706.
42. Dimmila P.A., Barbee K., Lauffenburger D.A. Mathematical model for the effects of adhesion and mechanics on cell migration speed. Biophys J. 60:1991;15-37.
43. Romer L.H., McLean N., Turner C.E., Burridge K. Tyrosine kinase activity, cytoskeletal organization, and motility in human vascular endothelial cells. Mol Biol Cell. 5:1994;349-361.
44. Richardson A., Parsons J.T. A mechanism for regulation of the adhesion-associated protein tyrosine kinase pp125FAK. Nature. 380:1996;538-540.
45. Illic D., Furuta Y., Kanazawa S., et al. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature. 377:1995;539-544.
46. Cary L.A., Chang J.F., Guan J.L. Stimulation of cell migration by overexpression of focal adhesion kinase and its association with Src and Fyn. J Cell Sci. 109:1996;1787-1794.