Properties of the methylcobalamin:H4folate methyltransferase involved in chloromethane utilization by Methylobacteriurn sp. strain CM4

European Journal of Biochemistry

Volumn 264, Issue 1, 1999, Pages 242-249

  Studer, Alex ^a   Vuilleumier, Stéphane ^a   Leisinger, Thomas ^a,b  

^a Institut für Mikrobiologie   (Switzerland)

^b ETH ZURICH   (Switzerland)

Author keywords

Chloromethane; Dehalogenation; Methylobacterium; Methyltransferase; Tetrahydrofol ate

Indexed keywords

5 METHYLTETRAHYDROFOLATE HOMOCYSTEINE METHYLTRANSFERASE; MECOBALAMIN; METHYL CHLORIDE; PRIMER DNA; PROTEIN METHYLTRANSFERASE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL METABOLISM; DEHALOGENATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; METHYLOBACTERIUM; PRIORITY JOURNAL; PROTEIN EXPRESSION; AMINO ACID SEQUENCE; CATALYSIS; ENZYMOLOGY; GRAM NEGATIVE AEROBIC RODS AND COCCI; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE HOMOLOGY; TEMPERATURE;

AMINO ACID SEQUENCE; BASE SEQUENCE; CATALYSIS; CHROMATOGRAPHY, ION EXCHANGE; DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GRAM-NEGATIVE AEROBIC BACTERIA; HYDROGEN-ION CONCENTRATION; METHYL CHLORIDE; MOLECULAR SEQUENCE DATA; PROTEIN O-METHYLTRANSFERASE; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE;

EID: 0033566374     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00629.x     Document Type: Article

References (29)

1. 1. Doronina, N.V., Sokolov, A.P. & Trotsenko, Y.A. (1996) Isolation and initial characterization of aerobic chloromethane-utilizing bacteria. FEMS Microbiol. Lett. 142, 179-183.
2. 2. Vannelli, T., Studer, A., Kertesz, M. & Leisinger, T. (1998) Chloromethane metabolism by Methylobacterium sp. strain CM4. Appl. Environ. Microbiol. 64, 1933-1936.
3. 3. Vannelli, T., Messmer, M., Studer, A., Vuilleumier, S. & Leisinger, T. (1999) A corrinoid-dependent catabolic pathway for growth of a Methylobacterium strain with chloromethane. Proc. Natl Acad. Sci. USA 96, 4615-4620.
4. 4. Burke, S.A., Lo, S.L. & Krzycki, J.A. (1998) Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine. J. Bacteriol. 180, 3432-3440.
5. 5. Harms, U. & Thauer, R.K. (1996) Methylcobalamin: coenzyme M methyltransferase isoenzymes MtaA and MtbA from Methanosarcina barkeri. Cloning, sequencing and differential transcription of the encoding genes, and functional overexpression of the mtaA gene in Escherichia coli. Eur. J. Biochem. 235, 653-639.
6. 6. Ferguson, D.J. Jr, Krzycki, J.A. & Grahame, D.A. (1996) Specific roles of methylcobamide: coenzyme M methyltransferase isozymes in metabolism of methanol and methylamines in Methanosarcina barkeri. J. Biol. Chem. 271, 5189-5194.
7. 7. Sauer, K., Harms, U. & Thauer, R.K. (1997) Methanol: coenzyme M methyltransferase from Methanosarcina barkeri. Purification, properties and encoding genes of the corrinoid protein MTI. Eur. J. Biochem. 243, 670-677.
8. 8. Kaufmann, F., Wohlfarth, G. & Diekert, G. (1998) O-demethylase from Acetobacterium dehalogenans: substrate specificity and function of the participating proteins. Eur. J. Biochem. 253, 706-711.
9. 9. Vorholt, J.A., Chistoserdova, L., Lidstrom, M.E. & Thauer, R.K. (1998) The NADP-dependent methylene tetrahydromethanopterin dehydrogenase in Methylobacterium extorquens AMI. J. Bacteriol. 180, 5351 -5356.
10. 10. Wohlfarth, G. & Diekert, G. (1997) Anaerobic dehalogenases. Curr. Opin. Biotechnol. 8, 290-295.
11. 11. Studier, F.W., Rosenberg, A.H., Dunn, J.J. & Dubendorff, J.W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185, 60-89.
12. 12. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (1999) Current Protocols in Molecular Biology. Greene Publishing Associates, New York.
13. 13. Wohlfarth, G., Geerligs, G. & Diekert, G. (1990) Purification and properties of a NADH-dependent 5,10-methylenetetrahydrofolate reductase from Peptostreptococcus productus. Eur. J. Biochem. 192, 411-417.
14. 14. Kreft, J.U. & Schink, B. (1994) O-demethylation by the homoacetogenic anaerobe Holophaga foetida studied by a new photometric methylation assay using electrochemically produced cob(I)alamin. Eur. J. Biochem. 226, 945-951.
15. 15. Messmer, M., Wohlfarth, G. & Diekert, G. (1993) Methyl chloride metabolism of strictly anaerobic, methyl chloride-utilizing homoacetogen strain MC. Arch. Microbiol. 160, 383-387.
16. 16. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
17. 17. Chistoserdova, L., Vorholt, J.A., Thauer, R.K. & Lidstrom, M.E. (1998) C1 transfer enzymes and coenzymes linking methylotrophic bacteria and methanogenic Archaea. Science 281, 99-102.
18. 18. Hogenkamp, H.P.C., Bratt, G.T. & Sun, S. (1985) Methyl transfer from methylcobalamin to thiols. A reinvestigation. Biochemistry 24, 6428-6432.
19. 19. LeClerc, G.M. & Grahame, D.A. (1996) Methylcobamide: coenzyme M methyltransferase isoenzymes from Methanosarcina barkeri. J. Biol. Chem. 271, 18725-18731.
20. 20. Corpet, F., Gouzy, J. & Kahn, D. (1999) Recent improvements of the ProDom database of protein domain families. Nucleic Acids Res. 27, 263-267.
21. 5-methyltetrahydromethanopterin: coenzyme M methyltransferase complex from Methanobacterium thermoautotrophicum is composed of eight different subunits. Eur. J. Biochem. 228, 640-648.
22. 12-Proteins (Kräutler, B., Arigoni, D. & Golding, B.T., eds), pp. 157-165. Wiley-VCH, Weinheim.
23. 23. Zhang, Z., Schäffer, A.A., Miller, W., Madden, T.L., Lipman, D.J., Koonin, E.V. & Altschul, S.F. (1999) Protein sequence similarity searches using patterns as seeds. Nucleic Acids Res. 26, 3986-3990.
24. 24. Goulding, C.W., Postigo, D. & Matthews, R.G. (1997) Cobalamin-dependent methionine synthase is a modular protein with distinct regions for binding homocysteine, methyltetrahydrofolate, cobalamin and adenosylmethionine. Biochemistry 36, 8082-8091.
25. 25. Roberts, D.L., Zhao, S., Doukov, T. & Ragsdale, S.W. (1994) The reductive acetyl coenzyme A pathway: sequence and heterologous expression of active methyltetrahydrofolate: corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum. J. Bacteriol. 176, 6127-6130.
26. 26. Zhao, S., Roberts, D.L. & Ragsdale, S.W. (1995) Mechanistic studies of the methyltransferase from Clostridium thermoaceticum: origin of the pH dependence of the methyl group transfer from methyltetrahydrofolate to the corrinoid/iron-sulfur protein. Biochemistry 34, 15075-15083.
27. 5-methyltetrahydromethanopterin: coenzyme M methyltransferase studied with cob(I)alamin as methyl acceptor and methylcob(III)alamin as methyl donor. Eur. J. Biochem. 226, 799-809.
28. 28. Cornish-Bowden, A. (1995) Fundamentals of Enzyme Kinetics. Portland Press, London.
29. 29. Hofmann, K., Bucher, P., Falquet. L. & Bairoch, A. (1999) The PROSITE database, its status in 1999. Nucleic Acids Res. 27, 215-219.