Induction of CD69 antigen on normal CD4+ and CD8+ lymphocyte subsets and its relationship with the phenotype of responding T-cells

Communications in Clinical Cytometry

Volumn 38, Issue 3, 1999, Pages 95-101

  Rutella, Sergio ^a,c   Rumi, C ^a   Lucia, M B ^a   Barberi, T ^a,b   Puggioni, P L ^a   Lai, M ^a   Romano, A ^a   Cauda, R ^a   Leone, G ^a  

^a CATHOLIC UNIVERSITY   (Italy)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^c CATHOLIC UNIVERSITY   (Italy)

Author keywords

Apoptosis; CD69; CD95; Lymphocyte activation

Indexed keywords

CD38 ANTIGEN; CD4 ANTIGEN; CD69 ANTIGEN; CD8 ANTIGEN; FAS ANTIGEN; INTERLEUKIN 2 RECEPTOR; LIPOCORTIN 5; PERFORIN; PHYTOHEMAGGLUTININ; T LYMPHOCYTE RECEPTOR;

ANTIGEN EXPRESSION; APOPTOSIS; ARTICLE; BLAST TRANSFORMATION; CELL PROLIFERATION; CELL SUBPOPULATION; CYTOTOXICITY; HUMAN; HUMAN CELL; LYMPHOCYTE ACTIVATION; MEMBRANE PERMEABILITY; PHENOTYPE; PRIORITY JOURNAL; T LYMPHOCYTE SUBPOPULATION;

ANNEXIN A5; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, T-LYMPHOCYTE; CD4-POSITIVE T-LYMPHOCYTES; CD8-POSITIVE T-LYMPHOCYTES; CELL MEMBRANE PERMEABILITY; CELLS, CULTURED; HUMANS; IMMUNOPHENOTYPING; MITOGENS; PHYTOHEMAGGLUTININS;

EID: 0033564671     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19990615)38:3<95::AID-CYTO1>3.0.CO;2-L     Document Type: Article

References (18)

1. Ziegler SF, Ramsdell F, Alderson MR. The activation antigen CD69. Stem Cells 1994;12:456-465.
2. Testi R, Phillips JH, Lanier LL. T cell activation via Leu-23 (CD69). J Immunol 1989;143:1123-1128.
3. Maino VC, Suni MA, Ruitenberg JJ. Rapid flow cytometric method for measuring lymphocyte subset activation. Cytometry 1995;20:127-133.
4. Perfetto SP, Hickey TE, Blair PJ, Maino VC, Wagner KF, Zhou S, Mayers DL, Louis DS, June CH, Siegel JN. Measurement of CD69 induction in the assessment of immune function in asymptomatic HIV-infected individuals. Cytometry 1997;30:1-9.
5. + peripheral blood progenitors are myeloperoxidase-negative and non-cycling irrespective of CD33 or CD13 coexpression. Exp Hematol 1997;25:246-251.
6. Bloemena E, Roos MTL, Van Heijst JLAM, Vossen JMJJ, Schellekens PTA. Whole-blood lymphocyte cultures. J Immunol Methods 1989;122: 161-167.
7. 8+ natural killer cell subset during HIV infection. Cytometry 1995;22:10-15.
8. Rutella S, Rumi C, Lucia MB, Sica S, Cauda R, Leone G. Serum of healthy donors receiving granulocyte colony-stimulating factor induces T-cell unresponsiveness. Exp Hematol 1998;26:1024-1033.
9. + cells of the CD4 and CD8 lineages. AIDS 1993;7:613-624.
10. Rumi C, Rutella S, Bonini S, Bonini St, Lambiase A, Sica S, Puggioni PL, Salutari P, Etuk B, Leone G. Immunophenotypic profile of peripheral blood eosinophils in acute graft-vs.-host disease. Exp Hematol 1998;26: 170-178.
11. Rutella S, Rumi C, Lucia MB, Etuk B, Cauda R, Leone G. Flow cytometric detection of perform in normal human lymphocyte subpopulations defined by expression of activation/differentiation antigens, Immunol Lett 1998;60:51-55.
12. Schmid I, Uittenbogaart CH, Keld B, Giorgi JV. A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser by flow cytometry. J Immunol Methods 1994;170:145-157.
13. van Engeland M, Nieland LJW, Ramaekers FCS, Schutte B, Reuteling-sperger CPM. Annexin V-affinity assay: a review on an apoptosis detection system based on phosphatidylserine exposure. Cytometry 1998;31:1-9.
14. Mahalingam M. Analysis of surface density of expression of molecules by flow cytometry [letter]. Cytometry 1996;24:190.
15. McGuinness D, Bennett S, Riley E. Statistical analysis of highly skewed immune response data. J Immunol Methods 1997;201:99-114.
16. Hamann J, Fiebig H, Strauss M. Expression cloning of the early activation antigen CD69, a type II integral membrane protein with a C-type lectin domain. J Immunol 1993;150:4920-4927.
17. Krowka JF, Cuevas B, Maron DC, Steimer KS, Ascher MA, Sheppard HW. Expression of CD69 after in vitro stimulation: a rapid method for quantitating impaired lymphocyte responses in HIV-infected individuals. J AIDS Hum Retrov 1996;11:95-104.
18. Kishimoto H, Surh CD, Sprent J. Upregulation of surface markers on dying thymocytes. J Exp Med 1995;181:649-655.