Compounds capable of generating singlet oxygen represent a source of artifactual data in scintillation proximity assays measuring phosphopeptide binding to SH2 domains

Analytical Biochemistry

Volumn 270, Issue 1, 1999, Pages 33-40

  Pai, James J K ^a   Kirkup, Michael P ^a   Frank, Emily A ^a   Pachter, Jonathan A ^a   Bryant, Robert W ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOTOSENSITIZERS; PROTEINS; SCINTILLATION; SODIUM AZIDE;

FALSE POSITIVE; FUSION PROTEINS; GLUTATHIONE S-TRANSFERASES; INDOLOCARBAZOLES; INHIBITORY ACTIVITY; PHOSPHOPEPTIDES; SINGLET OXYGEN; SRC HOMOLOGY 2 DOMAINS;

LIGHT;

CARBAZOLE DERIVATIVE; DITHIOTHREITOL; GLUTATHIONE TRANSFERASE; INDOLE DERIVATIVE; PHOSPHOPEPTIDE; PHOTOSENSITIZING AGENT; PROTEIN A; PROTEIN INHIBITOR; PYRAZOLONE DERIVATIVE; QUINOLONE DERIVATIVE; SINGLET OXYGEN; SODIUM AZIDE;

ANALYTIC METHOD; ANALYTICAL ERROR; ARTICLE; ARTIFACT REDUCTION; CHEMICAL STRUCTURE; CONCENTRATION RESPONSE; INCUBATION TIME; INHIBITION KINETICS; PHOTOSENSITIVITY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; SEQUENCE HOMOLOGY; VALIDATION PROCESS;

LETHRINIDAE;

EID: 0033562555     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4062     Document Type: Article

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