Flow cytometric analysis of the molecular mechanisms of immunosuppressive action of the active metabolite of leflunomide and its malononitrilamide analogues in a novel whole blood assay

Immunology Letters

Volumn 67, Issue 3, 1999, Pages 179-183

  Slauson, Stephen D ^a   Silva, Helio T ^a   Sherwood, Steven W ^a   Morris, Randall E ^a  

^a Dept Cardiothorac Surg S ^*  (United States)

Author keywords

CD134; CD25; Flow cytometry; Leflunomide; Malononitrilamide; Pyrimidine biosynthesis; Whole blood assay

Indexed keywords

2 CYANO 3 HYDROXY N [4 (TRIFLUOROMETHYL)PHENYL] 6 HEPTYNAMIDE; 2 CYANO N (4 CYANOPHENYL) 3 CYCLOPROPYL 3 OXOPROPIONAMIDE; BREQUINAR; CYCLOSPORIN; IMMUNOSUPPRESSIVE AGENT; LEFLUNOMIDE; MALONONITRILAMIDE DERIVATIVE; TERIFLUNOMIDE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONCENTRATION RESPONSE; CONTROLLED STUDY; FLOW CYTOMETRY; IMMUNOMODULATION; IMMUNOSUPPRESSIVE TREATMENT; LYMPHOCYTE PROLIFERATION; MALE; NONHUMAN; PRIORITY JOURNAL; PYRIMIDINE SYNTHESIS; RAT; T LYMPHOCYTE ACTIVATION;

ACRYLAMIDES; ALKYNES; ANILINE COMPOUNDS; ANIMALS; ANTIGENS, CD27; BIPHENYL COMPOUNDS; CYCLOSPORINE; DOSE-RESPONSE RELATIONSHIP, DRUG; FLOW CYTOMETRY; HEXANOIC ACIDS; HYDROXYBUTYRATES; IMMUNOSUPPRESSIVE AGENTS; ISOXAZOLES; LYMPHOCYTE ACTIVATION; LYMPHOCYTES; MALE; NITRILES; RATS; RATS, INBRED LEW; RECEPTORS, INTERLEUKIN-2; RECEPTORS, OX40; RECEPTORS, TUMOR NECROSIS FACTOR;

EID: 0033560704     PISSN: 01652478     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-2478(99)00007-3     Document Type: Article

References (13)

1. Silva H.T., Morris R.E. Am. J. Med. Sci. 313:1997;289-301.
2. Cherwinski H.M., McCarley D., Schatzman R., Devens B., Ransom J.T. J. Pharmacol. Exp. Ther. 272:1995;460-468.
3. Cherwinski H.M., Byars N., Ballaron S.J., Nakano G.M., Young J.M., Ransom J.T. Inflamm. Res. 44:1995;317-322.
4. Dimitrijevic M., Bartlett R.R. Inflamm. Res. 45:1996;550-556.
5. Kurrle R., Bartlett R., Ruuth E., Laufler L., Schorlemmer H.U. Transplant. Proc. 28:1996;3053-3056.
6. Bloemena E., Koopmans R.P., Weinreich S., van Boxtel C.J., Schellekens P.T.A. Clin. Immunol. Immunopathol. 57:1990;374-386.
7. Romeu M.A., Mestre M., Gonzales L., Valls A., Verdaguer J., Corominas M., Bas J., Massip E., Buendia E. J. Immunol. Methods. 154:1992;7-10.
8. De Groote D., Gevaert Y., Lopez M., Gathy R., Fauchet F., Dehart I., Jadoul M., Radoux D., Franchimont P. J. Immunol. Methods. 163:1993;259-267.
9. Metcalfe S., Sveesen R., Calne R. Transplant. Int. 5:1992;S514-S515.
10. Paterson D.J., Jefferies W.A., Green J.R., Brandon M.R., Corthesy P., Puklavek M., Williams A.F. Mol. Immunol. 24:1987;1281-1290.
11. Godfrey W.R., Fagoni F.F., Harara M.A., Buck D., Engleman E.G. J. Exp. Med. 180:1994;757-762.
12. Calderhead D.M., Buhlmann J.E., van den Eatwegh A.J.M., Claassen E., Noelle R.J., Fell H.P. J. Immunol. 151:1993;5261-5271.
13. Slauson S., Silva H., Shorthouse R., Cao W., Morris R.E. FASEB J. 12:1998;621.