The kinetics of acylation and deacylation of penicillin acylase from Escherichia coli ATCC 11105: Evidence for lowered pK(a) values of groups near the catalytic centre

Biochemical Journal

Volumn 338, Issue 1, 1999, Pages 235-239

  Morillas, Manuel ^a   Goble, Martin L ^a   Virden, Richard ^a  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

Author keywords

Pre steady state kinetics; Stopped flow spectrophotometry

Indexed keywords

ACETIC ACID DERIVATIVE; AMIDASE; BACTERIAL ENZYME; PENICILLIN G;

ACYLATION AND DEACYLATION; ARTICLE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DISSOCIATION CONSTANT; ENZYME KINETICS; ESCHERICHIA COLI; IONIZATION; NONHUMAN; PRIORITY JOURNAL;

ACYLATION; CATALYSIS; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; NITROPHENOLS; PENICILLIN AMIDASE; SPECTROPHOTOMETRY; TIME FACTORS;

ARRHENIUS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; FELIS CATUS;

EID: 0033558174     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3380235     Document Type: Article

References (24)

1. Böck, A., Wirth, R., Schmid, G., Schumacher, G., Lang, G. and Buckel, P. (1983) FEMS Microbiol. Lett. 20, 141-144
2. Schumacher, G., Sizmann, D., Haug, H., Buckel, P. and Böck, A. (1986) Nucleic Acids Res. 14, 5713-5727
3. Kutzbach, C. and Rauenbusch, E. (1974) Hoppe-Seyler's Z. Physiol. Chem. 354, 45-53
4. Slade, A., Horrocks, A. J., Lindsay, C. D., Dunbar, B. and Virden, R. (1991) Eur. J. Biochem. 197, 75-80
5. Martín, J., Slade, A., Aitken, A., Arche, R. and Virden, R. (1991) Biochem. J. 280, 659-662
6. Choi, K. S., Kim, J. A. and Kang, H. S. (1992) J. Bacteriol. 174, 6270-6276
7. Duggleby, H. J., Tolley, S. P., Hill, C. P., Dodson, E. J., Dodson, G. and Moody, P. C. E. (1995) Nature (London) 373, 264-268
8. Brannigan, J. A., Dodson, G., Duggleby, H. J., Moody, P. C. E., Smith, J. L., Tomchick, D. R. and Murzin, A. G. (1995) Nature (London) 378, 416-419; erratum p. 644
9. Roa, A., Castillon, M. P., Goble, M. L., Virden, R. and García, J. L. (1995) Biochem. Biophys. Res. Commun. 206, 629-636
10. Roa, A., Goble, M. L., García, J. L., Acebal, C. and Virden, R. (1996) Biochem. J. 316, 409-412
11. Ellis, K. J. and Morrison, J. F. (1987) Methods Enzymol. 87, 405-426
12. Gill, S. C. and von Hippel, P. H. (1989) Anal. Biochem. 182, 319-326
13. Huggins, C. and Lapides, J. (1947) J. Biol. Chem. 170, 467-482
14. Duggleby, R. G. (1985) Biochem. J. 228, 55-60
15. Gutfreund, H. and Sturtevant, J. M. (1956) Biochem. J. 63, 656-661
16. Margolin, A. L., Svedas, V. K. and Berezin, I. V. (1980) Biochim. Biophys. Acta 616, 283-289
17. Cleland, W. W. (1967) Adv. Enzymol. 29, 1-32
18. Shvyadas, V. Y., Margolin, A. L., Sherstyuk, S. F., Klesov, A. A. and Berezin, I. V. (1977) Bioorg. Khim. 3, 546-554
19. Fersht, A. R. (1974) Proc. R. Soc. London Ser. B 187, 397-407
20. Martín, J., Prieto, I., Barbero, J. L., Perez-Gil, J., Mancheño, J. M. and Arche, R. (1990) Biochim. Biophys. Acta 1037, 133-139
21. Wang, C.-L., Calvo, K. C. and Klapper, M. H. (1981) Biochemistry 20, 1401-1408
22. Martín, J., Prieto, I., J., Mancheño, J. M., Barbero, J. L., and Arche, R. (1993) Biotechnol. Appl. Biochem. 17, 311-325
23. Tanford, C. (1962) Adv. Protein Chem. 17, 69-165
24. Gruen, L., Laskowski, Jr., M. and Scheraga, H. A. (1959) J. Am. Chem. Soc. 81, 3891-3901