Rapid purification of membrane extrinsic F1-domain of chloroplast ATP synthase in monodisperse form suitable for 3D-crystallization

European Journal of Biochemistry

Volumn 260, Issue 1, 1999, Pages 15-21

  Groth, Georg ^a   Schirwitz, Katja ^b  

^a HEINRICH HEINE UNIVERSITY   (Germany)

^b NONE

Author keywords

Crystal; F ATPase; Perfusion chromatography; Purification

Indexed keywords

AMMONIUM SULFATE; CALCIUM ION; DITHIOTHREITOL; MAGNESIUM ION; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; RIBULOSEBISPHOSPHATE CARBOXYLASE;

AMINO ACID SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CATALYSIS; CHLOROPLAST; CONTROLLED STUDY; CRYSTALLIZATION; ENZYME ACTIVITY; ENZYME PURIFICATION; IMMUNOBLOTTING; ISOELECTRIC FOCUSING; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; SILVER STAINING; SPINACH; THYLAKOID MEMBRANE;

ADENOSINE DIPHOSPHATE; CALCIUM; CHLOROPLASTS; CHROMATOGRAPHY, ION EXCHANGE; CRYSTALLIZATION; DITHIOTHREITOL; ENZYME ACTIVATION; ISOELECTRIC POINT; KINETICS; MAGNESIUM; MEMBRANE PROTEINS; MOLECULAR WEIGHT; NUCLEOTIDES; PLANT PROTEINS; PROTON-TRANSLOCATING ATPASES; SPINACIA OLERACEA;

SPINACIA OLERACEA;

EID: 0033557433     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00101.x     Document Type: Article

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