Wheat-germ aspartate transcarbamoylase: Revised purification, stability and re-evaluation of regulatory kinetics in terms of the Monod-Wyman-Changeux model

European Journal of Biochemistry

Volumn 259, Issue 1-2, 1999, Pages 71-78

  Khan, Ahsan I ^a   Chowdhry, Babur Z ^a   Yon, Robert J ^a  

^a UNIVERSITY OF GREENWICH   (United Kingdom)

Author keywords

Allosteric; Enzyme kinetics; Purification; Uridine monophosphate; Wheat germ aspartate transcarbamoylase

Indexed keywords

ASPARTATE CARBAMOYLTRANSFERASE; CARBAMOYL PHOSPHATE; GLYCEROL; URIDINE PHOSPHATE;

ALLOSTERISM; ARTICLE; CONTROLLED STUDY; DISSOCIATION CONSTANT; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STABILITY; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; TEMPERATURE; WHEAT GERM;

ALLOSTERIC REGULATION; ASPARTATE CARBAMOYLTRANSFERASE; CARBAMYL PHOSPHATE; ENZYME STABILITY; KINETICS; MODELS, CHEMICAL; SEEDS; TRITICUM; URIDINE MONOPHOSPHATE;

TRITICUM AESTIVUM;

EID: 0033555734     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00005.x     Document Type: Article

References (31)

1. Gerhart, J.C. & Pardee, A.B. (1964) Aspartate transcarbamylase, an enzyme designed for feedback inhibition. Fed. Proc. Am. Soc. Exp. Biol. 23, 727-735.
2. Allewell, N.M. (1989) Escherichia coli aspartate transcarbamoylase: structure, energetics, and catalytic and regulatory mechanisms. Annu. Rev. Biophys. Chem. 18, 72-91.
3. Hervé, G. (1989) Aspartate transcarbamylase from E. coli. In Allosteric Enzymes (Hervé, G. ed.), pp. 61-79, CRC Press, Boca Raton, FL.
4. Stevens, R.C. & Lipscomb, W.N. (1992) A molecular mechanism for pyrimidine and purine nucleotide control of aspartate transcarbamoylase. Proc. Natl. Acad. Sci. USA 89, 5281-5285.
5. Lipscomb, W.N. (1994) Aspartate transcarbamylase from Escherichia coli- activity and regulation. Advan. Enzymol. 68, 67-151.
6. Hervé, G., Nagy, M., Le Gouar, M., Penveme, B. & Ladjimi, M. (1993) The carbamoyl phosphate synthetase aspartate transcarbamoylase complex of Saccharomyces cerevisiae: molecular and cellular aspects. Biochem. Soc. Trans. 24, 195-198.
7. Wild, J.R. & Wales, M.E. (1990) Molecular evolution and genetic-engineering of protein domains involving aspartate transcarbamoylase. Annu. Rev. Microbiol. 44, 193-218.
8. Davidson, J.N., Chen, K.C., Jamison, R.S. & Musmanno, L.A. & Kern, C.B. (1993) The evolutionary history of the first three enzymes in pyrimidine biosynthesis. Bioessays 15, 157-164.
9. Williamsun, C.L. & Slocum, R.D. (1994) Molecular cloning and chracterization of the pyrB1 and pyrB2 genes encoding aspartate transcarbamoylase in pea (Pisum sativum L.). Plant Physiol. 105, 377-384.
10. Monod, J., Wyman, J. & Changeux, J.-P. (1965) On the nature of allosteric transitions: a plausible model. J. Mol. Biol. 12, 88-118.
11. Yon, R.J. (1989) A contrast in allostery: aspartate transcarbamoylases from wheat and from Escherichia coli. Biochem. Soc. Trans. 17, 564-565.
12. Wiley, D.C. & Lipscomb, W.N. (1968) Crystallographic determination of symmetry of aspartate transcarbamylase. Nature 218, 1119-1121.
13. Yon, R.J., Grayson, J.E., Chawda, A. & Butterworth, P.J. (1982) The quarternary structure of wheat-germ aspartate transcarbamoylase. Biochem. J. 203, 413-417.
14. Cole, S.C.J. & Yon, R.J. (1989) Preliminary comparison of catalytic chains of aspartate transcarbamoylases from wheat and Escherichia coli. Biochem. Soc. Trans. 17, 565-566.
15. Bartlett, T.J., Aibangbee, A., Bruce, I.J & Donovan, P.J. (1994) Endogenous polypeptide chain length and partial sequence of aspartate transcarbamoylase from wheat, characterized by immunochemical and cDNA methods. Biochim. Biophys. Acta 1207, 187-193.
16. Yon, R.J. (1981) Versatility of mixed-function adsorbents in biospecific protein desorption: accidental affinity and an improved purification of aspartate transcarbamoylase from wheat germ. Anal. Biochem. 113, 219-228.
17. Yon, R.J. (1984) Regulatory kinetics of wheat-germ aspartate transcarbamoylase. Adaptation of the concerted model to account for complex kinetic effects of uridine 5′-monophosphate. Biochem. J. 221, 281-287.
18. Khan, A., Chowdhry, B.Z. & Yon, R.J. (1996) Effects of lipids on nucleotide inhibition of wheat-germ aspartate transcarbamoylase: an additional level of control? Biochem. J. 313, 669-673.
19. Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
20. Drake, A.F. (1994) Measurements of electron absorption spectra in the UV and visible. In Methods in Molecular Biology (Jones, C., Mulloy, B. & Thomas, A.H., eds), Vol. 22, pp. 173-182, Humana Press, Totowa, NJ.
21. Kohn, J. & Wilchek, M. (1982) A new approach (cyano-transfer) for cyanogen-bromide activation of Sepharose at neutral pH, which yields activated resins, free of interfering nitrogen derivatives. Biochem. Biophys. Res. Commun. 107, 878-884.
22. Yon, R.J. & Simmonds, R.J. (1975) Protein chromatography on adsorbents with hydrophobic and ionic groups. Some properties of N-(3-carboxypropionyl) aminodecyl-Sepharose and its interaction with wheat-germ aspartate transcarbamoylase, Biochem. J. 151, 281-290.
23. Grayson, J.E., Yon, R.J. & Butterworth, P.J. (1979) Wheat-germ aspartate transcarbamoylase - purification and cold-lability. Biochem. J. 183, 239-245.
24. Cornish-Bowden, A. (1976) Principles of Enzyme Kinetics, pp. 175-180. Butterworths, London and Boston.
25. Rubin, M.M, & Changeux, J.-P. (1966) On the nature of allosteric transitions: implications of non-exclusive ligand-binding. J. Mol. Biol. 21, 265-274.
26. Wyman, J. & Gill, S.J. (1990) Binding and Linkage: Functional Chemistry of Biological Molecules, pp. 134-137, University Science Books, Mill, Valley, CA.
27. Yon, R.J. (1972) Wheat-germ aspartate transcarbamoylase - kinetic behavior suggesting an allosteric mechanism of regulation. Biochem. J. 128, 311-320.
28. Grayson, J.E. & Yon, R.J. (1978) Wheat germ aspartate transcarbamoylase: reversible, ligand-dependent aggregation behaviour in vivo. Biochem. Soc. Trans. 6, 197-200.
29. Porter, R.W., Modebe, M.O. & Stark, G.R. (1969) Aspartate transcarbamylase: kinetic studies of the catalytic subunit. J. Biol. Chem. 244, 1846-1859.
30. Suter, P. & Rosenbusch, J.P. (1977) Asymmetry of binding and physical assignments of CTP and ATP sites in aspartate transcarbamoylase. J. Biol. Chem. 252, 8136-8141.
31. Gibbons, I., Ritchey, J.M. & Schachman, H.K. (1976) Concerted allosteric transition in hybrids of aspartate transcarbamoylase containing different arrangements of active and inactive sites. Biochemistry 15, 1324-1330.