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Volumn 38, Issue 1-2, 1999, Pages 135-137

Differences between 4'-RNA and 4'-DNA radicals during anaerobic and aerobic strand cleavage

Author keywords

DNA cleavage; Nucleotides; Radical ions; RNA

Indexed keywords

DNA; RNA;

EID: 0033555210     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1521-3773(19990115)38:1/2<135::AID-ANIE135>3.0.CO;2-U     Document Type: Article
Times cited : (22)

References (18)
  • 8
    • 0345143273 scopus 로고    scopus 로고
    • note
    • The stereochemistry at C4′ of nucleotide 11 was determined by NOE experiments. A medium-sized NOE effect was observed between 5′-H and 3′-H, a small NOE effect between C4′-(acetoxymethyl) and 1′-H, as well as between the methyl group of the acetate and one of the methyl groups of the isopropylidene protecting group.
  • 9
    • 0344280908 scopus 로고    scopus 로고
    • note
    • Phosphite 13 was activated with pivaloyl chloride, subsequently coupled with the oligonucleotide bound to the solid phase, and the oligonucleotide synthesis was finally performed with a synthesizer.
  • 12
    • 0344712844 scopus 로고    scopus 로고
    • These experiments were carried out as described in ref. [8]
    • These experiments were carried out as described in ref. [8].
  • 13
    • 0031048783 scopus 로고    scopus 로고
    • In model studies with noncharged phosphate triesters in alcoholic solutions the effect of the 2′-OH group is considerably larger: D. Crich, X. Mo, J. Am. Chem. Soc: 1997, 119, 245. This shows how risky it is to extrapolate results obtained from model reactions with small molecules in organic solvents to explain biomolecules in water.
    • (1997) J. Am. Chem. Soc , vol.119 , pp. 245
    • Crich, D.1    Mo, X.2
  • 16
    • 0345143272 scopus 로고    scopus 로고
    • note
    • 2. After photolyzing for 5-10 min (Osram 500 W, 320 nm filter, 25°C) the products were separated by HPLC on a reversed-phase column and the peaks were analyzed by MALDI-TOF MS. The compounds 5′-phosphate 2 and 3′-phosphoglycolate 7 were obtained in all experiments together with 2-8% of 3′-phosphate.
  • 17
    • 11544259897 scopus 로고    scopus 로고
    • The preferred formation of 3′-phosphoglycolate 7 can also be explained by the Burger mechanism in which the cleavage of intermediate 6a in DNA leads preferentially to 7, see R. M. Burger, Chem. Rev. 1998, 98, 1153.
    • (1998) Chem. Rev. , vol.98 , pp. 1153
    • Burger, R.M.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.