Suppression of an actin-binding protein, drebrin, by antisense transfection attenuates neurite outgrowth in neuroblastoma B104 cells

Developmental Brain Research

Volumn 114, Issue 2, 1999, Pages 193-200

  Toda, Masahiro ^a   Shirao, Tomoaki ^a,b   Uyemura, Keiichi ^a  

^a KEIO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b GUNMA UNIVERSITY   (Japan)

Author keywords

Antisense; Drebrin; Neurite outgrowth; Stable transfectant; Suppression

Indexed keywords

ACTIN BINDING PROTEIN; COMPLEMENTARY DNA; CYTOSKELETON PROTEIN; DREBRIN; GANGLIOSIDE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; GENETIC TRANSFECTION; NERVE FIBER GROWTH; NEUROBLASTOMA CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SERUM;

ANIMALS; CELL DIVISION; DNA, COMPLEMENTARY; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; NEURITES; NEUROBLASTOMA; NEUROPEPTIDES; OLIGODEOXYRIBONUCLEOTIDES, ANTISENSE; RATS; TRANSFECTION; TUMOR CELLS, CULTURED;

ANIMALIA;

EID: 0033553537     PISSN: 01653806     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-3806(99)00030-9     Document Type: Article

References (34)

1. Asada H., Uyemura K., Shirao T. Actin-binding protein, drebrin accumulates in submembranous regions in parallel with neuronal differentiation. J. Neurosci. Res. 38:1994;149-159.
2. Bernhardt R., Huber G., Matus A. Differences in the developmental patterns of three microtubule-associated proteins in the rat cerebellum. J. Neurosci. 5:1985;977-991.
3. Bernstein B.W., Bamburg J.R. Tropomyosin binding to F-actin protects the F-actin from disassembly by brain actin-depolymerizing factor (ADF). Cell Motil. 2:1982;1-8.
4. Fan J., Mansfield S.G., Redmond T., Gordon-Weeks P.R., Raper J.A. The organization of F-actin and microtubules in growth cones exposed to brain-derived collapsing factor. J. Cell Biol. 121:1993;867-878.
5. Faulstich H., Trischmann H., Mayer D. Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosis. Exp. Cell Res. 144:1983;73-82.
6. Ferreira A., Busciglio J., Caceres A. An immunocytochemical analysis of the ontogeny of the microtubule associated proteins MAP-2 and tau in the nervous system of the rat. Dev. Brain Res. 34:1987;9-31.
7. Fifkova E. A possible mechanism of morphometric changes in dendritic spines induced by stimulation. Cell. Mol. Neurobiol. 5:1985;47-63.
8. Horio T., Hotani H. Visualization of the dynamic instability of individual microtubules by dark-field microscopy. Nature. 321:1986;605-607.
9. Ikeda K., Shirao T., Toda M., Asada H., Toya S., Uyemura K. Effect of neuron-specific actin-binding protein, drebrin A, on cell-substratum adhesion. Neurosci. Lett. 194:1995;197-200.
10. Inouye M. Antisense RNA: its function an application in gene regulation - a review. Gene. 72:1988;25-34.
11. Ishikawa R., Hayashi K., Shirao T., Xue Y., Takagi T., Sasaki Y.et al. Drebrin, a development-associated brain protein from rat embryo, causes the dissociation of tropomyosin from actin filaments. J. Biol. Chem. 269:1994;29928-29933.
12. Ishikawa R., Yamashiro S., Matsumura F. Annealing of gelsolin-severed actin fragments by tropomyosin in the presence of Ca2+. Potentiation of the annealing process by caldesmon. J. Biol. Chem. 264:1989;12764-16770.
13. Katoh K., Takahashi Y., Hayashi S., Kondoh H. Improved mammalian vectors for high expression of G418 resistance. Cell Struct. Funct. 12:1987;575-580.
14. Knecht D.A., Loomis W.F. Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum. Science. 236:1987;1081-1086.
15. Kojima N., Kato Y., Shirao T., Obata K. Nucleotide sequences of two embryonic drebrins, developmental change in their mRNAs. Mol. Brain Res. 464:1988;207-215.
16. Kojima N., Shirao T., Obata K. Molecular cloning of a developmentally regulated brain protein, chicken drebrin A and its expression by alternative splicing of the drebrin gene. Mol. Brain Res. 19:1993;101-114.
17. Legrand C., Ferraz C., Clavel M.C., Rabie A. Immunocytochemical localization of gelsolin in oligodendroglia of the developing rabbit central nervous system. Brain Res. 395:1986;231-235.
18. Liu T., Williams J.G., Clarke M. Inducible expression of calmodulin antisense RNA in Dictyostelium cells inhibits the completion of cytokinesis. Mol. Biol. Cell. 3:1992;1403-1413.
19. Morgan J.I., Seifert W. Growth factors and gangliosides: a possible new perspective in neuronal growth control. J. Supramol. Struct. 10:1979;111-124.
20. Moriyama K., Nishida E., Yonezawa N., Sakai H., Matsumoto S., Iida K.et al. Destrin, a mammalian actin-depolymerizing protein, is closely related to cofilin. Cloning and expression of porcine brain destrin cDNA. J. Biol. Chem. 265:1990;5768-5773.
21. Neely M.D., Gesemann M. Disruption of microfilaments in growth cones following depolarization and calcium influx. J. Neurosci. 14:1994;7511-7520.
22. Sasaki Y., Hayashi K., Shirao T., Ishikawa R., Kohama K. Inhibition by drebrin of the actin-bundling activity of brain fascin, a protein localized in filopodia of growth cones. J. Neurochem. 66:1996;980-988.
23. Schubert D., Heinemann S., Carlisle W., Tarikas H., Kimes B., Patrick et al J. Clonal cell lines form the rat central nervous system. Nature. 249:1974;224-227.
24. Shirao T. The role of microfilament-associated proteins, drebrins, in brain morphogenesis: a review. J. Biochem. 117:1995;231-236.
25. Shirao T., Hayashi K., Ishikawa R., Isa K., Asada H., Ikeda et al K. Formation of thick, curving bundles of actin by drebrin A expressed in fibroblasts. Exp. Cell Res. 215:1994;145-153.
26. Shirao T., Kojima N., Obata K. Cloning of drebrin A and induction of neurite-like processes in drebrin-transfected cells. NeuroReport. 3:1992;109-112.
27. Shirao T., Obata K. Two acidic proteins associated with brain development in chick embryo. J. Neurochem. 44:1985;1210-1216.
28. Shirao T., Obata K. Immunochemical homology of 3 developmentally regulated brain proteins and their developmental change in neuronal distribution. Dev. Brain Res. 29:1986;233-244.
29. Toda M., Miura M., Asou H., Toya S., Uyemura K. Cell growth suppression of astrocytoma C6 cells by glial fibrillary acidic protein cDNA transfection. J. Neurochem. 63:1994;1975-1978.
30. Toda M., Shirao T., Minoshima S., Shimizu S., Toya S., Uyemura K. Molecular cloning of cDNA encoding human drebrin E and chromosomal mapping of its gene. Biochem. Biophys. Res. Commun. 196:1993;468-472.
31. Tucker R.P. The roles of microtubule-associated proteins in brain morphogenesis: a review. Brain Res. Rev. 15:1990;101-120.
32. Varnum-Finney B., Reichardt L.F. Vinculin-deficient PC12 cell lines extend unstable lamellipodia and filopodia and have a reduced rate of neurite outgrowth. J. Cell Biol. 127:1994;1071-1084.
33. Weinstein D.E., Shelanski M., Liem R.K.H. Suppression by antisense mRNA demonstrates a requirement for the glial fibrillary acidic protein in the formation of stable astrocytic processes in response to neurons. J. Cell Biol. 112:1991;1205-1213.
34. Wigler M., Pellicer A., Silverstein S., Axel R., Urlaub G., Chasin L. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 76:1979;1373-1376.