Increased helix and protein stability through the introduction of a new tertiary hydrogen bond

Journal of Molecular Biology

Volumn 286, Issue 5, 1999, Pages 1609-1619

  Peterson, Ronald W ^a   Nicholson, Eric M ^a   Thapar, Roopa ^b   Klevit, Rachel E ^b   Scholtz, J Martin ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Hydrogen bonds; N capping; NMR; Protein engineering; Protein stability

Indexed keywords

CARRIER PROTEIN; GLUTAMIC ACID; LYSINE; SERINE;

ALPHA HELIX; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CHEMICAL PROCEDURES; CONTROLLED STUDY; HYDROGEN BOND; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; THERMAL EXPOSURE;

EID: 0033548543     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.2574     Document Type: Article

References (24)

1. Anderson J. W., Bhanot P., Georges F., Klevit R. E., Waygood E. B. Involvement of the carboxy-terminal residue in the active site of the histidine-containing protein, HPr, of the phosphoenolpyruvate:sugar transport system of Escherichia coli. Biochemistry. 30:1991;9601-9607.
2. 15N with side-chain H beta resonances in larger proteins. J. Magn. Reson. 95:1991;636-641.
3. Aurora R., Rose G. D. Helix capping. Protein Sci. 7:1998;21-38.
4. Bai Y., Milne J. S., Mayne L., Englander S. W. Primary structure effects on peptide group hydrogen exchange. Proteins: Struct. Funct. Genet. 17:1993;75-86.
5. Becktel W. J., Schellman J. A. Protein stability curves. Biopolymers. 26:1987;1859-1877.
6. Blaber M., Baase W. A., Gassner N., Matthews B. W. Alanine scanning mutagenesis of the alpha-helix 115-123 of phage T4 lysozyme: effects on structure, stability and the binding of solvent. J. Mol. Biol. 246:1995;317-330.
7. Byrne M. P., Manuel R. L., Lowe L. G., Stites W. E. Energetic contribution of side-chain hydrogen bonding to the stability of staphylococcal nuclease. Biochemistry. 34:1995;13949-13960.
8. 1H coupling constants. J. Magn. Reson. 107:1995;172-178.
9. Cavanagh J., Palmer A. G. III, Wright P. E., Rance M. J. Sensitivity improvement in proton-detected two-dimensional heteronuclear relay spectroscopy. J. Magn. Reson. 91:1991;429.
10. elMasry N. F., Fersht A. R. Mutational analysis of the N-capping box of the alpha-helix of chymotrypsin inhibitor 2. Protein Eng. 7:1994;777-782.
11. Harper E. T., Rose G. D. Helix stop signals in proteins and peptides: the capping box. Biochemistry. 32:1993;7605-7609.
12. Ho S. N., Hunt H. D., Horton R. M., Pullen J. K., Pease L. R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene. 77:1989;51-59.
13. Loh S. N., Markley J. L. Hydrogen bonding in proteins as studied by amide hydrogen D/H fractionation factors: application to staphylococcal nuclease. Biochemistry. 33:1994;1029-1036.
14. Marqusee S., Sauer R. T. Contributions of a hydrogen bond/salt bridge network to the stability of secondary and tertiary structure in λ repressor. Protein Sci. 3:1994;2217-2225.
15. Myers J. K., Pace C. N. Hydrogen bonding stabilizes globular proteins. Biophys. J. 71:1996;2033-2039.
16. Nicholson E. M., Scholtz J. M. Conformational stability of the Escherichiacoli HPr protein: test of the linear extrapolation method and a thermodynamic characterization of cold denaturation. Biochemistry. 35:1996;11369-11378.
17. 15N spectroscopy. J. Magn. Reson. 87:1990;488-501.
18. Pace C. N. Determination and analysis of urea and guanidine hydrochloride denaturation curves. Methods Enzymol. 131:1986;266-280.
19. Palmer A. G. III, Cavanagh J., Wright P. E., Rance M. J. Sensitivity improvement in proton-detected two-dimensional heteronuclear correlation NMR spectroscopy. J. Magn. Reson. 93:1991;151-170.
20. Scholtz J. M. Conformational stability of HPr: the histidine-containing phosphocarrier protein from Bacillus subtilis. Protein Sci. 4:1995;35-43.
21. Spera S., Ikura M., Bax A. Measurement of exchange rates of rapidly exchanging amide protons: application to the study of calmodulin and its complex with myosin light chain kinase fragment. J. Biomol. NMR. 1:1991;155-165.
22. Thapar R., Nicholson E. M., Waygood E. B., Scholtz J. M., Klevit R. E. Influence of N-cap mutations on the structure and stability of E. coli HPr. Biochemistry. 35:1996;11268-11277.
23. Wishart D. S., Sykes B. D., Richards F. M. Relationship between nuclear magnetic resonance chemical shift and protein secondary structure. J. Mol. Biol. 222:1991;311-333.
24. Zhukovsky E. A., Mulkerrin M. G., Presta L. G. Contribution to global protein stabilization of the N-capping box in human growth hormone. Biochemistry. 33:1994;9856-9864.