A p85 subunit-independent p110α PI 3-kinase colocalizes with p70 s6 kinase on actin stress fibers and regulates thrombin-stimulated stress fiber formation in: Swiss 3T3 cells

Experimental Cell Research

Volumn 248, Issue 1, 1999, Pages 223-233

  Johanson, Sven O ^a   Naccache, Paul A ^b   Crouch, Michael F ^a  

^a Australian National University   (Australia)

^b LAVAL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

2 MORPHOLINO 8 PHENYLCHROMONE; GROWTH FACTOR; PHOSPHATIDYLINOSITOL 3 KINASE; S6 KINASE; THROMBIN; WORTMANNIN;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CELL STRUCTURE; CONTROLLED STUDY; ENZYME ACTIVITY; GOLGI COMPLEX; MICROTUBULE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

ANIMALIA;

EID: 0033541441     PISSN: 00144827     EISSN: None     Source Type: Journal    
DOI: 10.1006/excr.1999.4405     Document Type: Article

References (30)

1. Crouch M. F., Hendry I. A. Growth factor second messenger systems: Oncogenes and the heterotrimeric GTP-binding protein connection. Med. Res. Rev. 13:1993;105-123.
2. 2. Curr. Opin. Cell Biol. 7:1995;183-189.
3. Ridley A. J. Signal transduction through the GTP-binding proteins Rac and Rho. J. Cell Sci. Suppl. 18:1994;127-131.
4. Ridley A. J., Hall A. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell. 70:1992;389-399.
5. Ridley A. J., Hall A. Signal transduction pathways regulating Rho-mediated stress fibre formation: Requirements for a tyrosine kinase. EMBO J. 13:1994;2600-2610.
6. Higaki M., Sakaue H., Ogawa W., Kasuga M., Shimokado K. Phosphatidylinositol 3-kinase-dependent signal transduction pathway for platelet-derived growth factor-induced chemotaxis. J. Biol. Chem. 271:1996;29342-29346.
7. v3. J. Invest Dermatol. 107:1996;597-602.
8. γ1. Circ. Res. 78:1996;312-321.
9. Nobes C. D., Hawkins P., Stephens L., Hall A. Activation of the small GTP-binding proteins rho and rac by growth factor receptors. J. Cell Sci. 108:1995;225-233.
10. Martin S. S., Rose D. W., Saltiel A. R., Klippel A., Williams L. T., Olefsky J. M. Phosphatidylinositol 3-kinase is necessary and sufficient for insulin-stimulated stress fiber breakdown. Endocrinology. 137:1996;5045-5054.
11. Crouch M. F. Regulation of thrombin-induced stress fibre formation in Swiss 3T3 cells by the 70-kDa S6 kinase. Biochem. Biophys. Res. Commun. 233:1997;193-199.
12. S6k. Nature. 370:1994;71-75.
13. Vanhaesebroeck B., Leevers S. J., Panayotou G., Waterfield M. D. Phosphoinositide 3-kinases: A conserved family of signal transducers. Trends Biochem. Sci. 22:1997;267-272.
14. Carpenter C. L., Cantley L. C. Phosphoinositide kinases. Curr. Opin. Cell Biol. 8:1996;153-158.
15. Stoyanov B., Volinia S., Hanck T., Rubio L., Loubtchenkov M., Malek D., Stoyanova S., Vanhaesebroeck B., Dhand R., Nürnberg B., Gierschik P., Seedorf K., Hsuan J. J., Waterfield M. D., Wetzker R. Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase. Science. 269:1995;690-693.
16. βγ. Cell. 89:1997;105-114.
17. iα. FASEB J. 5:1991;200-206.
18. Endemann G., Dunn S. N., Cantley L. C. Bovine brain contains two types of phosphatidylinositol kinase. Biochemistry. 26:1987;6845-6852.
19. Rodriguez-Viciana P., Warne P. H., Khwaja A., Marte B. M., Pappin D., Das P., Waterfield M. D., Ridley A., Downward J. Role of phosphoinositide 3-OH kinase in cell transformation and control of the actin cytoskeleton by Ras. Cell. 89:1997;457-467.
20. Monfar M., Lemon K. P., Grammer T. C., Cheatham L., Chung J., Vlahos C. J., Blenis J. Activation of pp70/85 S6 kinases in interleukin-2-responsive lymphoid cells is mediated by phosphatidylinositol 3-kinase and inhibited by cyclic AMP. Mol. Cell Biol. 15:1995;326-337.
21. Kilgour E., Gout I., Anderson N. G. Requirement for phosphoinositide 3-OH kinase in growth hormone signalling to the mitogen-activated protein kinase and p70s6k pathways. Biochem. J. 315:1996;517-522.
22. Wilson M., Burt A. R., Milligan G., Anderson N. G. Wortmannin-sensitive activation of p70s6k by endogenous and heterologously expressed Gi-coupled receptors. J. Biol. Chem. 271:1996;8537-8540.
23. Kucera G. L., Rittenhouse S. E. Human platelets form 3-phosphorylated phosphoinositides in response to α-thrombin, U46619, or GTPγS. J. Biol. Chem. 265:1990;5345-5348.
24. Domin J., Dhand R., Waterfield M. D. Binding to the platelet-derived growth factor receptor transiently activates the p85α-p110α phosphoinositide 3-kinase complexin vivo. J. Biol. Chem. 271:1996;21614-21621.
25. Zhang J., Benovic J. L., Sugai M., Wetzker R., Gout I., Rittenhouse S. E. Sequestration of a G-protein βγ subunit or ADP-ribosylation of Rho can inhibit thrombin-induced activation of platelet phosphoinositide 3-kinases. J. Biol. Chem. 270:1995;6589-6594.
26. IIb3. J. Biol. Chem. 271:1996;6265-6272.
27. Woscholski R., Dhand R., Fry M. J., Waterfield M. D., Parker P. J. Biochemical characterization of the free catalytic p110α and the complexed heterodimeric p110α · p85α forms of the mammalian phosphatidylinositol 3-kinase. J. Biol. Chem. 269:1994;25067-25072.
28. Klippel A., Reinhard C., Kavanaugh W. M., Apell G., Escobedo M. A., Williams L. T. Membrane localization of phosphatidylinositol 3-kinase is sufficient to activate multiple signal-transducing kinase pathways. Mol. Cell Biol. 16:1996;4117-4127.
29. Otsu M., Hiles I., Gout I., Fry M. J., Ruiz L. F., Panayotou G., Thompson A., Dhand R., Hsuan J., Totty N. Characterization of two 85 kd proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI3-kinase. Cell. 65:1991;91-104.
30. Pons S., Asano T., Glasheen E., Miralpeix M., Zhang Y., Fisher T. L., Myers-MG J., Sun X. J., White M. F. The structure and function of p55PIK reveal a new regulatory subunit for phosphatidylinositol 3-kinase. Mol. Cell Biol. 15:1995;4453-4465.