Differing roles for the diiron clusters of ribonucleotide reductase from aerobically grown Escherichia coli in the generation of the Y122 radical [5]

Journal of the American Chemical Society

Volumn 121, Issue 5, 1999, Pages 1096-1097

  Miller, Marcia A ^a   Gobena, Feben T ^a   Kauffmann, Karl ^b   Münck, Eckard ^b   Que Jr , Lawrence ^a   Stankovich, Marian T ^a  

^a University of Minnesota   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; RIBONUCLEOTIDE REDUCTASE;

BACTERIAL GROWTH; CATALYSIS; DNA SYNTHESIS; ENZYME ACTIVITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; LETTER; NONHUMAN; STOICHIOMETRY;

EID: 0033540715     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9826845     Document Type: Letter

References (24)

1. Stubbe, J. Adv. Enymol. 1990, 63, 349-419.
2. Reichard, P. Annu. Rev. Biochem. 1995, 64, 1-28.
3. Sjöberg, B.-M. Struct. Bonding 1997, 88, 140-173.
4. Uhlin, U.; Eklund, H. Nature 1994, 370, 533-539.
5. Nordlund, P.; Sjöberg, B.-M.; Eklund, H. Nature 1990, 345, 393-398.
6. Nordlund, P.; Eklund, H. J. Mol. Biol. 1993, 232, 123-164.
7. Larsson, A.; Sjöberg, B.-M. EMBO J. 1986, 5, 2037-2040.
8. Elgren, T. E.; Lynch, J. B.; Juarez-Garcia, C.; Münck, E.; Sjöberg, B.-M.; Que, L., Jr. J. Biol. Chem. 1991, 266, 19265-19268.
9. Bollinger, J. M., Jr.; Tong, W. H.; Ravi, N.; Huynh, B. H.; Edmondson, D. E.; Stubbe, J. J. Am. Chem. Soc. 1994, 116, 8015-8023.
10. Ochiai, E.-I.; Mann, G. J.; Gräslund, A.; Thelander, L. J. Biol. Chem. 1990, 265, 15758-15761.
11. Bollinger, J. M., Jr.; Edmondson, D. E.; Huynh, B. H.; Filley, J.; Norton, J.; Stubbe, J. Science 1991, 253, 292-298.
12. Lynch, J. B.; Juarez-Garcia, C.; Münck, E.; Que, L., Jr. J. Biol. Chem. 1989, 264, 8091-8096.
13. Scarrow, R. C.; Maroney, M. J.; Palmer, S. M.; Roe, A. L.; Que, L., Jr.; Salowe, S. P.; Stubbe, J. J. Am. Chem. Soc. 1987, 109, 7857-7864.
14. Atkin, C.; Thelander, L.; Reichard, P.; Lang, G. J. Biol. Chem. 1973, 248, 7464-7472.
15. Petersson, L.; Gräslund, A.; Ehrenberg, A.; Sjöberg, B.-M.; Reichard, P. J. Biol. Chem. 1980, 255, 6706-6712.
16. Paulsen, K. E.; Liu, Y.; Fox, B. B.; Lipscomb, J. D.; Münck, E.; Stankovich, M. T. Biochemistry 1994, 33, 713-722.
17. slow ∼ 5-7). Han, J.-Y.; Swarts, J. C.; Sykes, A. G. Inorg. Chem. 1996, 35, 4629-4634.
18. 1/2 = 2300 s). This suggests that the rates of the fast and slow phases differ by about 2 orders of magnitude.
19. II.
20. Bollinger, J. M., Jr.; Tong, W. H.; Ravi, N.; Huynh, B. H.; Edmondson, D. E.; Stubbe, J. J. Am. Chem. Soc. 1994, 116, 8024-8032.
21. Parkin, S. E.; Chen, S.; Ley, B. A.; Mangravite, L.; Edmondson, D. E.; Huynh, B. H.; Bollinger, J. M., Jr. Biochemistry 1998, 37, 1124-1130.
22. Tong, W.; Burdi, D.; Riggs-Gelasco, P.; Chen, S.; Edmondson, D.; Huynh, B. H.; Stubbe, J.; Han, S.; Arvai, A.; Tainer, J. Biochemistry 1998, 37, 6086-6094.
23. Electron transfer may occur between diiron clusters in the same dimer via a transfer pathway across subunits yet to be uncovered or between clusters of R2 dimers via the proposed pathway from the active site to the surface of the protein, (a) Sjöberg, B.-M. Structure 1994, 2, 793-796. (b) Schmidt, P. P.; Rova, U.; Katterles, B.; Thelander, L.; Gräslund, A. J. Biol. Chem. 1998, 273, 21463-21472.
24. Electron transfer may occur between diiron clusters in the same dimer via a transfer pathway across subunits yet to be uncovered or between clusters of R2 dimers via the proposed pathway from the active site to the surface of the protein, (a) Sjöberg, B.-M. Structure 1994, 2, 793-796. (b) Schmidt, P. P.; Rova, U.; Katterles, B.; Thelander, L.; Gräslund, A. J. Biol. Chem. 1998, 273, 21463-21472.