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The term oligomer refers to monodisperse compounds. Material with a molecular weight distribution is designated as polymer.
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Single-channel activity in patch-clamp experiments with OHBs could only be observed when a voltage of ca. 100 mV was applied (see ref 8b).
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note
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In 1, the molar ratio of 1 to POPC used during the liposome preparation was 1:250. After gel permeation chromatography, 1/POPC in the liposome fractions was 1:240 (corresponding to an incorporation yield of ca. 95%). For 2, liposomes were prepared with 2/POPC = 1:500, yielding 1:430 in the liposome fractions (corresponding to 85%). Based on these observations with 1 and 2, a mean incorporation yield of 90% was assumed and taken into account for 3-6.
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0346292907
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note
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Please note that for all vesicle preparations used, gel permeation chromatography followed the ultrasound treatment.
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54
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0348183601
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note
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This procedure was performed at room temperature or 50°C and also in the presence of 10% (v/v) methanol. The incorporation rate could not be increased by using these different experimental conditions.
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This type of interaction has been discussed before in work on synthetic flux-promoting compounds
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0346923006
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In this context, the term pore refers to a geometrical arrangement, without implying any classification of transport mechanisms.
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note
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In analogy to the postulation that at least three 32-mers form a pore by arranging six helices (see Figure 8), we suppose that two 64-mers (corresponding to eight helices) were involved in the active carrier species. It might be that not all "transmembrane helices" are involved in the pore formation.
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68
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Cantor, C. R., Ed.; Springer-Verlag: New York
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For example, see: (a) Osman, P.; Cornell, B. Biochim. Biophys. Acta 1994, 1195, 197. (b) Ludtke, S. J.; He, K.; Heller, W. T.; Harroun, T. A.; Yang, L.; Huang, H. W. Biochemistry 1996, 35, 13723.
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Cornell, B.2
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For example, see: (a) Osman, P.; Cornell, B. Biochim. Biophys. Acta 1994, 1195, 197. (b) Ludtke, S. J.; He, K.; Heller, W. T.; Harroun, T. A.; Yang, L.; Huang, H. W. Biochemistry 1996, 35, 13723.
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