Identification of the RNA binding domain of T4 RegA protein by structure-based mutagenesis

Journal of Biological Chemistry

Volumn 274, Issue 45, 1999, Pages 32265-32273

  Gordon, Johnthan ^a   Sengupta, Tapas K ^a   Phillips, Christine A ^a,c   O'Malley, Shawn M ^b   Williams, Kenneth R ^a,b   Spicer, Eleanor K ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b YALE UNIVERSITY   (United States)

^c PPDI Parmaco Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MUTANT PROTEIN; PROTEIN;

ARTICLE; BINDING AFFINITY; BINDING SITE; MOLECULAR MODEL; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN RNA BINDING; PROTEIN STRUCTURE; RNA BINDING;

BACTERIOPHAGE T4; BINDING SITES; CIRCULAR DICHROISM; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; RNA; STRUCTURE-ACTIVITY RELATIONSHIP; VIRAL PROTEINS;

EID: 0033527557     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.274.45.32265     Document Type: Article

References (35)

1. Winter, R. B., Morrissey, L., Gauss, P., Gold, L., Hsu, T., and Karam, J. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 7822-7826
2. Webster, K. W., Adari, H. Y., and Spicer, E. K. (1989) Nucleic Acids Res. 17, 10047-10068
3. Unnithan, S., Green, L., Morrissey, L., Binkley, J., Singer, B., Karam, J., and Gold, L. (1990) Nucleic Acids Res. 18, 7083-7092
4. Webster, K. R., and Spicer, E. K. (1990) J. Biol. Chem. 285, 19007-19014
5. Karam, J., Gold, L., Singer, B. S., and Dawson, M. (1981) Proc. Natl. Acad. Sci. U. S. A. 78, 4669-4673
6. Kang, C.-H., Chan, R., Berger, L, Lockshin, C., Green, L., Gold, L., and Rich, A. (1995) Science 268, 1170-1173
7. Phillips, C. A., Gordon, J., and Spicer, E. K. (1996) Nucleic Acids Res. 24, 4319-4326
8. Webster, K. R., Keill, S., Konigsberg, W., Williams, K. R., and Spicer, E. K. (1992) J. Biol. Chem. 267, 26097-26103
9. O'Malley, S. M., Sattar, A. K. M., Williams, K. R., and Spicer, E. K. (1995) J. Biol. Chem. 270, 5107-5114
10. Rould, M. A., Perona, J. J., Söll, D., and Steitz, T. A. (1989) Science 246, 1135-1142
11. Valegard, K., Murray, J. B., Stockley, P. G., Stonehouse, N. J., and Liljas, L. (1994) Nature 371, 623-626
12. Oubridge, C., Ito, N., Evans, P. R., Teo, C-H., and Nagai, K. (1994) Nature 372, 432-438
13. Birney, E., Kumar, S., and Krainer, A. R. (1993) Nucleic Acids Res. 21, 5803-5816
14. Burd, C. G., and Dreyfuss, G. (1994) Science 265, 615-621
15. Shamoo, Y, Krueger, U., Rice, L. M., Williams, K. R., and Steitz, T. A. (1997) Nat. Struct. Biol. 4, 215-222
16. Webster, K. W., Shamoo, Y., Konigsberg, W. H., and Spicer, E. K. (1991) BioTechniques 11, 658-661
17. Adari, H. Y., Rose, K., Williams, K. W., Konigsberg, W. H., Lin, T.-C., and Spicer, E. K. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 1901-1905
18. McSwiggen, J. A., Bear, D. G., and Von Hippel, P. H. (1988) J. Mol. Biol. 199, 609-622
19. Johnson, B. H., and Hecht, M. H. (1994) Bio/Technology 12, 114-117
20. Sambrook, J., Fritach, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., p. 5.86, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
21. Rebar, E. J., and Pabo, C. O. (1994) Science 263, 671-673
22. Fried, M. G., and Crothers, D. M. (1983) Nucleic Acids Res. 11, 141-158
23. Jessen, T. H., Oubridge, C., Teo, C. H., Pritchard, C., and Nagai, K. (1991) EMBO J. 10, 3447-3456
24. Jozwik, C. E., and Miller, E. S. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 5053-5057
25. Allen, S. V., and Miller, E. S. (1999) Anal. Biochem. 269, 32-37
26. Nagai, K., Oubridge, C., Jessen, T. H., Li, J., and Evans, P. R. (1990) Nature 348, 515-520
27. Fersht, A. R., Shi, J.-P., Knill-Jones, J., Lowe, D. M., Wilkinson, A. J., Blow, D. M., Brick, P., Carter, P., Waye, M. M. Y., and Winter, G. (1985) Nature 314, 235-238
28. Brandon, C., and Tooze, J. (1998) Introduction to Protein Structure, 2nd Ed., pp. 129-147, Garland Publishing, New York
29. Tan, R., Chen, L., Buettner, J. A., Hudson, D., and Frankel, A. D. (1993) Cell 73, 1031-1040
30. Jain, C., and Belasco, J. G. (1996) Cell 87, 115-125
31. Draper, D. E. (1995) Annu. Rev. Biochem. 64, 593-620
32. Hall, K. B. (1994) Biochemistry 33, 10076-10088
33. Allain, F. H.-T., Gubser, C. C., Howe, P. W. A., Nagai, K., Neuhaus, D., and Varani, G. (1996) Nature 380, 646-650
34. Battiste, J. L., Mao, H., Rao, N. S., Tan, R., Muhandiram, D. R., Kay, L. E., Frankel, A. D., and Williamson, J. R. (1996) Science 273, 1547-1551
35. Szewczak, A. A., Webster, K. R, Spicer, E. K., and Moore, P. B. (1991) J. Biol. Chem. 266, 17832-17837