Monitoring of intracellular ribonucleotide pools is a powerful tool in the development and characterization of mammalian cell culture processes

Biotechnology and Bioengineering

Volumn 64, Issue 3, 1999, Pages 357-367

  Grammatikos, Stefanos I ^a   Tobien, Kai ^a   Noé, Wolfgang ^a   Werner, Rolf G ^a  

^a BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

Author keywords

Cell physiology; Chinese hamster ovary; Large scale cell culture; NTP U ratio; Nucleotide triphosphates

Indexed keywords

BIOREACTORS; CYTOLOGY; FERMENTATION; GROWTH KINETICS; PHOSPHATES; PHYSIOLOGY; RNA;

NUCLEOTIDE TRIPHOSPHATES; RIBONUCLEOTIDE;

ANIMAL CELL CULTURE;

RIBONUCLEOTIDE; URIDINE DIPHOSPHATE; URIDINE TRIPHOSPHATE;

ANIMAL CELL; ARTICLE; BIOREACTOR; BIOTECHNOLOGY; CELL CULTURE; CELL VIABILITY; CHO CELL; FERMENTATION; GENETIC RECOMBINATION; NONHUMAN; NUCLEOTIDE METABOLISM;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; HYBRIDOMAS; RIBONUCLEOTIDES; TIME FACTORS;

ANIMALIA; CRICETINAE; CRICETULUS GRISEUS; MAMMALIA;

EID: 0033527086     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990805)64:3<357::AID-BIT12>3.0.CO;2-R     Document Type: Article

References (16)

1. Al-Rubeai M, Emery AN, Chalder S, Jan DC. 1992. Specific monoclonal antibody productivity and the cell-cycle comparisons of batch, continuous and perfusion cultures. Cytotechnol 9:85-97.
2. Atkinson DE. 1969. Regulation of enzyme function. Annu Rev Microbiol 23:47-68.
3. Barnabé N, Butler M. 1994. Effects of temperature on nucleotide pools and monoclonal antibody production in a mouse hybridoma. Biotechnol Bioeng 44:1235-1245.
4. Constantinidis I, Sambanis A. 1998. Noninvasive monitoring of tissue-engineered constructs by nuclear magnetic resonance methodologies. Tissue Eng 4:9-17.
5. Grammatikos SI, Valley U, Nimtz M, Conradt HS, Wagner R. 1998. Intracellular UDP-N-acetylhexosamine pool affects N-glycan complexity: A mechanism of ammonium action on protein glycosylation. Biotechnol Prog 14:410-419.
6. Jenkins N, Hovey A. 1993. Temperature control of growth and productivity in mutant Chinese hamster ovary cells synthesizing a recombinant protein. Biotechnol Bioeng 42:1029-1036.
7. Marlin SD, Staunton DE, Springer TA, Stratowa C, Sommergruber W, Merluzzi VJ. 1990. A soluble form of intercellular adhesion molecule-1 inhibits rhinovirus infection. Nature 344:70-74.
8. Moore A, Mercer J, Dutina G, Donahue CJ, Bauer KD, Mather JP, Etcheverry T, Ryll T. 1997. Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultures. Cytotechnol 23:47-54.
9. 13C NMR studies. Biochim Biophys Acta 1052:255-263.
10. Ryll T, Jäger V, Wagner R. 1991a. Intracellular concentration of ATP and other nucleotides during continuous cultivation of hybridoma cells. In: Spier RE, Griffiths JB, Meignier B, editors. Production of biologicals from animal cells in culture. London: Butterworths. p 236-242.
11. Ryll T, Jäger V, Wagner R. 1991b. Variation of ratios and concentrations of nucleotide triphosphate and UDP-sugars during a perfused batch cultivation of hybridoma cells. In: Sasaki R, Ikura K, editors. Animal cell culture and production of biologicals. Dordrecht, The Netherlands: Kluwer. p 307-317.
12. Ryll T, Wagner R. 1991. Improved ion-pair high-performance liquid chromatographic method for the quantification of a wide variety of nucleotides and sugar-nucleotides in animal cells. J Chromatogr 570:77-88.
13. Ryll T, Wagner R. 1992. Intracellular ribonucleotide pools as a tool for monitoring the physiological state of in vitro cultivated mammalian cells during production processes. Biotechnol Bioeng 40:934-946.
14. Ryll T, Valley U, Wagner R. 1994. Biochemistry of growth inhibition by ammonium ions in mammalian cells. Biotechnol Bioeng 44:184-193.
15. Schwabe JO, Pörtner R. 1998. Process control and on-line feeding strategies for fed-batch and dialysis cultures of hybridoma cells. In: Merten O-W, Perrin P, Griffiths B, editors. New Developments and new applications in animal cell technology. Dordrecht, The Netherlands: Kluwer. p 347-349.
16. Staunton DE, Merluzzi VJ, Rothlein R, Barton R, Marlin SD, Springer TA. 1989. A cell adhesion molecule, ICAM-1, is the major surface receptor for rhinoviruses. Cell 56:849-856.