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Volumn 9, Issue 13, 1999, Pages 1745-1750

Substrate-selective mechanisms in biocatalysis demonstrated with a versatile and efficient aldolase antibody

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; FRUCTOSE BISPHOSPHATE ALDOLASE;

EID: 0033526885     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(99)00292-9     Document Type: Article
Times cited : (6)

References (38)
  • 1
    • 0003905731 scopus 로고
    • Morrison, J. D., Ed.; Academic: New York
    • 1. For reviews on the aldol reaction, see: (a) Heathcock, C. H. In Asymmetric Synthesis; Morrison, J. D., Ed.; Academic: New York, 1984; Vol. 3.
    • (1984) Asymmetric Synthesis , vol.3
    • Heathcock, C.H.1
  • 6
  • 8
    • 0000851696 scopus 로고
    • Trost, B. M., Ed.; Pergamon: Oxford
    • (h) Heathcock, C. H. In Comprehensive Organic Synthesis; Trost, B. M., Ed.; Pergamon: Oxford, 1991; Vol. 2, pp 133-319.
    • (1991) Comprehensive Organic Synthesis , vol.2 , pp. 133-319
    • Heathcock, C.H.1
  • 20
    • 0013605060 scopus 로고    scopus 로고
    • Submitted for publication
    • 5. Shulman, A.; Keinan, E., Submitted for publication.
    • Shulman, A.1    Keinan, E.2
  • 22
    • 0013631291 scopus 로고    scopus 로고
    • note
    • 7. Cinnamaldehydes (R = Cl, OMe, CF3) were prepared from the appropriately substituted cinnnamic acid in a three-step sequence: esterification, DIBAL-H reduction to the corresponding alcohol, and PCC oxidation to the desired aldehydes.
  • 23
    • 0013582153 scopus 로고    scopus 로고
    • note
    • 8. All antibody-catalyzed reactions were performed in phosphate buffered saline (50 mM phosphate, 100 mM, NaCl pH 7.4) containing 10% acetonitrile. The progress of the reactions was monitored by HPLC and initial rates were calculated by regressional analysis. Antibody concentrations were between 4-8 uM active sites. Initial rates were measured within the first 1 h of the reaction. The uncatalyzed reactions were found to be too slow to be determined under these reaction times and conditions.
  • 29
    • 0013628735 scopus 로고    scopus 로고
    • note
    • 13. All reactions were carried out in carbonate buffer (25 mM, pH 10.05) containing 10% acetonitrile. The progress of the reactions was monitored by HPLC. Substrate, relative rate: 1a, 1.6; 1b, 1.3; 1c, 1.5; 1d, 1.1; 1e, 1.4; 1f, 1.0.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.