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note
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7. Cinnamaldehydes (R = Cl, OMe, CF3) were prepared from the appropriately substituted cinnnamic acid in a three-step sequence: esterification, DIBAL-H reduction to the corresponding alcohol, and PCC oxidation to the desired aldehydes.
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23
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0013582153
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note
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8. All antibody-catalyzed reactions were performed in phosphate buffered saline (50 mM phosphate, 100 mM, NaCl pH 7.4) containing 10% acetonitrile. The progress of the reactions was monitored by HPLC and initial rates were calculated by regressional analysis. Antibody concentrations were between 4-8 uM active sites. Initial rates were measured within the first 1 h of the reaction. The uncatalyzed reactions were found to be too slow to be determined under these reaction times and conditions.
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29
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0013628735
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note
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13. All reactions were carried out in carbonate buffer (25 mM, pH 10.05) containing 10% acetonitrile. The progress of the reactions was monitored by HPLC. Substrate, relative rate: 1a, 1.6; 1b, 1.3; 1c, 1.5; 1d, 1.1; 1e, 1.4; 1f, 1.0.
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