Enzymatic synthesis of oligopeptides in mixed reverse micelles

Tetrahedron Letters

Volumn 40, Issue 10, 1999, Pages 1971-1974

  Xing, Guo wen ^a   Liu, Dao jun ^a   Ye, Yun hua ^a   Ma, Ji ming ^a  

^a PEKING UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CHYMOTRYPSIN A; HEPTANE; OLIGOPEPTIDE; WATER;

ARTICLE; MICELLE; PEPTIDE ANALYSIS; PEPTIDE SYNTHESIS; REACTION ANALYSIS; REACTION TIME; STRUCTURE ANALYSIS;

EID: 0033525662     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(99)00126-4     Document Type: Article

References (12)

1. Martinek, K.; Klyachko, N.L.; Kabanov, A.V.; Khmelnitsky, Y.L.; Levashov, A.V. Biochim. Biophys. Acta. 1989, 981, 161-172.
2. Menger, F.M.; Yamada, K. J. Am. Chem. Soc. 1979, 101, 6731-6734.
3. Barbaric, S.; Luisi, P.L. J. Am. Chem. Soc. 1981, 103, 4239-4244.
4. Luthi, P.; Luisi, P.L. J. Am. Chem. Soc. 1984, 106, 7285-7286.
5. Jorba, X.; Clapes, P.; Torres, J.L.; Valencia, G.; Mata-Alvarez, J. Colloids Surf. A 1995, 96, 47-52.
6. Chen, Y.X.; Zhang, X.Z.; Zheng, K.; Chen, S.M.; Wang, Q.C.; Wu, X.X. Enzyme Microb. Technol. 1998, 23, 243-248.
7. Tian, G.L.; Liu,Y.; Wang, H.; Ye, Y.H. Chem. J. Chin. Univ. 1996, 17, 55-59.
8. Xing, G.W.; Tian, G.L.; Lu, Y.; Ye, Y.H. In Peptides: Biology and Chemistry (Proceedings of the 1996 Chinese Peptide Symposium); Xu, X.J.; Ye, Y.H.; Tam, J.P. Eds.; Kluwer Academic Publishers: The Netherlands, 1998; pp. 50-51.
9. Ye, Y.H.; Tian, G.L.; Xing, G.W.; Dai, D.C.; Chen, G.; Li, C.X. Tetrahedron, 1998, 54, 12585-12596.
10. AOT: bis(2-ethylhexyl) sodium sulfosuccinate, Brij30: polyoxyethylene(4) lauryl ether.
11. Khmelnitsky, Y.L.; Kabanov, A.V.; Klyachko, N.L.; Levashov, A.V.; Martinek, K. Enzymatic catalysis in reversed micelles. In Structure and Reactivity in Reverse Micelles; Pileni, M.P., Ed.; Eisevier: Amsterdam, 1989; pp. 230-261.
12. 3N and 54μ1 water solution of α-chymotrypsin (10mg/ml) were added. After the substrate was completely dissolved by shaking on a vibromixer, 0.25mmol (85mg) ZTyrOEt was immediately added. The observed pH value of the solution was about 10.0 which was determined by dropping a small amount of the mixture on a damp pH paper. The mixture was stirred at room temperature for 24 hours. At the end of the reaction, the precipitate was filtered and washed with 1M hydrochloric acid (4ml × 2) and water (4ml × 2). The product was obtained in 86% yield.