Altered substrate selectivity in a mutant of an intrahelical salt bridge in UhpT, the sugar phosphate carrier of Escherichia coli

Journal of Biological Chemistry

Volumn 274, Issue 10, 1999, Pages 6148-6153

  Hall, Jason A ^a   Fann, Mon Chou ^a   Maloney, Peter C ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; CARRIER PROTEIN; CYSTEINE; GLUCOSE 6 PHOSPHATE; LYSINE; PROTEIN UHPT; SUGAR PHOSPHATE; SUGAR PHOSPHATE CARRIER PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CARBOHYDRATE TRANSPORT; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CARRIER PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MOLECULAR SEQUENCE DATA; MONOSACCHARIDE TRANSPORT PROTEINS; MUTATION; SUBSTRATE SPECIFICITY; SUGAR PHOSPHATES;

EID: 0033525543     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.274.10.6148     Document Type: Article

References (52)

1. Maloney, P. C., Ambudkar, S. V., Anantharam, V., Sonna, L. A., and Varadhachary, A. (1990) Microbiol. ReV. 54, 1-17
2. Maloney, P. C., and Wilson, T. H. (1996) in Escherichia coli and Salmonella: Cellular and Molecular Biology (Neidhardt, F. C., Curtiss, R., Ingraham, J. L., Lin, E. C. C., Low, K. B., Magasanik, B., Reznikoff, W. S., Riley, M., Schaechter, M., and Umbarger, H. E., eds) pp. 1130-1148, American Society of Microbiology, Washington, D. C.
3. Sonna, L. A., Ambudkar, S. V., and Maloney, P. C. (1988) J. Biol. Chem. 263, 6625-6630
4. Pao, S. S., Paulsen, I. T., and Saier, M. H. (1998) Microbiol. Mol. Biol. Rev. 62, 1-34
5. Marger, M. D., and M. H. Saier (1993) Trends Biochem. Sci. 18, 13-20
6. Henderson, P. J. F. (1993) Curr. Opin. Cell Biol. 5, 708-721
7. Goswitz, V. C., and Brooker, R. J. (1995) Protein Sci. 4, 534-537
8. Venkatesan, P., and Kaback, H. R. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 9802-9807
9. Varela, M. F., and Wilson, T. H. (1996) Biochim. Biophys. Acta 1276, 21-34
10. Rashin, A. A., and Honig, B. (1984) J. Mol. Biol. 173, 515-521
11. Honig, B. H., and Hubbell, W. L. (1984) Proc. Natl. Acad. Sci. U. S. A. 81, 5412-5416
12. Scholtz, J. M., Qian, H., Robbins, V. H., and Baldwin, R. L. (1993)Biochemistry 32, 9668-9676
13. Marqusee, S., and Sauer, R. T. (1994) Protein Sci. 3, 2217-2225
14. Stern, L. J., and Khorana, H. G. (1989) J. Biol. Chem. 264, 14202-14208
15. Lounnas, V., and Wade, R. C. (1997) Biochemistry 36, 5402-5417
16. Lee, J.-I., Varela, M. F., and Wilson, T. H. (1996) Biochim. Biophys. Acta 1278, 111-118
17. Cain, B. D., and Simoni, R. D. (1988) J. Biol. Chem. 263, 6606-6612
18. Kaback, H. R., Frillingos, S., Jung, H., Jung, K., Prive, G. G., Ujwal, M. L., Weitzman, C., Wu, J., and Zen, K. (1994) J. Exp. Biol. 196, 183-195
19. Weston, L. A., and Kadner, R. J. (1987) J. Bacteriol. 169, 3546-3555
20. Tamai, E., Fann, M.-C., Tsuchiya, T., and Maloney, P. C. (1997) Protein Expression Purif. 10, 275-282
21. Yan, R.-T., and Maloney, P. C. (1993) Cell 75, 37-44
22. Ho, S. N., Hunt, H. D., Morton, R. M., Pullen, J. K., and Pease, L. R. (1989) Gene (Amst.) 77, 51-59
23. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 488-492
24. Miller, J. H. (1972) Experiments in Molecular Genetics, p. 431, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
25. Laemmli, U. K. (1970) Nature 227, 680-685
26. Harlow, E., and Lane, D. (1988) Antibodies: A Laboratory Manual, pp. 486-510, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
27. Matos, M., Fann, M.-C., Yan, R.-T., and Maloney, P. C. (1996) J. Biol. Chem. 271, 18571-18575
28. Fann, M.-C., Davies, A. H., Varadhachary, A., Kuroda, T., Sevier, C., Tsuchiya, T., and Maloney, P. C. (1998) J. Membr. Biol. 164, 187-195
29. Maloney, P. C., Ambudkar, S. V., Thomas, J., and Schiller L. (1984) J. Bacteriol. 158, 238-245
30. Friedrich, M. J., and Kadner, R. J. (1987) J. Bacteriol. 169, 3556-3563
31. Island, M. D., Wei, B.-Y., and Kadner, R. J. (1992) J. Bacteriol. 174, 2754-2762
32. Gott, P., and Boos, W. (1988) Mol. Microbiol. 2, 655-663
33. Cairns, J., Overbaugh, J., and Miller, S. (1988) Nature 335, 142-145
34. Foster, P. L. (1993) Annu. Rev. Microbiol. 47, 467-504
35. Saier, M. H., Jr., Wentzel, D. L., Feucht, B. U., and Judice, J. J. (1975) J. Biol. Chem. 250, 5089-5096
36. Varadhachary, A., and Maloney, P. C. (1991) J. Biol. Chem. 266, 130-135
37. Ambudkar, S. V., Larson, T. J., and Maloney, P. C. (1986) J. Biol. Chem. 261, 9083-9086
38. Elvin, C. M., Hardy, C. M., and Rosenberg, H. (1985) J. Bacteriol. 161, 1054-1058
39. Hofstee, B. H. J. (1959) Nature 184, 1296-1298
40. Dixon, M. (1953) Biochem. J. 55, 170-171
41. Wilson, D. M., and Wilson, T. H. (1992) J. Bacteriol. 174, 3083-3086
42. Panayotova-Heiermann, M., Loo, D. D. F., Lam, J. T., and Wright, E. M. (1998) Biochemistry 37, 10522-10528
43. King, S. C., Hansen, C. L., and Wilson, T. H. (1991) Biochim. Bipophys. Acta 1062, 177-186
44. Ambudkar, S. V., Sonna, L. A., and Maloney, P. C. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 280-284
45. Brooker, R. J., and Wilson, T. H. (1985) Proc. Natl. Acad. Sci. U. S. A. 82, 3959-3963
46. King, S. C., and Wilson, T. H. (1990) J. Biol. Chem. 265, 9638-9644
47. Olsen, S. G., Greene, K. M., and Brooker, R. J. (1993) J. Bacteriol 175, 6269-6275
48. Niiya, S., Yamasaki, K., Wilson, T. H., and Tsuchiya, T. (1982) J. Biol. Chem. 257, 8902-8906
49. Botfield, M. C., and Wilson, T. H. (1988) J. Biol. Chem. 263, 12909-12915
50. Merino, G., and Shuman, H. A. (1997) J. Bacteriol. 179, 7687-7694
51. Yan, R.-T., and Maloney, P. C. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 5973-5976
52. Andersson, S. G. E., Zomorodipour, A., Andersson, J. O., Sicheritz-Ponten, T., Alsmark, U. C. M., Podowski, R. M., Nauslund, A. K., Eriksson, A.-S., Winkler, H. H., and Kurland, C. G. (1998) Nature 396, 133-140