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18O isotopes) allowed us to conclude that the product with the carboxamide-terminus (16a) was in large excess compared to the one with the carboxyterminus upon incubation of 14a in rat brain homogenate, but it was formed in a small proportion in rat plasma and rat liver homogenate. The ratio of the amide (16a, monoisotopic nominal m/z 547) to the acid (m/z 548) after 30 min of incubation in brain homogenate was calculated to be 4.3:1, while the ratio in plasma and liver homogenate after 60 min of incubation was 1:6.3 and 1:6.7, respectively.
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See Supporting Information (Figure S-2) for details.
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Yoo, O.1
Powell, C.2
Argarwal, K.3
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Characterization of cDNA for precursor of human luteinizing hormone releasing hormone
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Seeburg, P.; Adelman, J. Characterization of cDNA for precursor of human luteinizing hormone releasing hormone. Nature 1984, 311, 666-668.
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(1984)
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Seeburg, P.1
Adelman, J.2
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0344476488
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note
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See Supporting Information (Figure S-3) for chromatogram and mass spectra.
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54
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0344045348
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note
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It has been noted that a significant difference in analeptic activity was obtained between the targeting system containing both Pro and Ala as spacer (13c and 13f). Although this observation could not be rationalized based on our prior knowledge about the affinity of the enzymes possibly involved in the cleavage toward the peptide bonds of the respective substrates, its detailed study was beyond the scope of this paper.
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55
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Proposal for a common nomenclature for sequence ions in mass-spectra of peptides
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Roepstorff, P.; Fohlman, J. Proposal for a common nomenclature for sequence ions in mass-spectra of peptides. Biomed. Mass Spectrom. 1984, 11, 601.
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Roepstorff, P.1
Fohlman, J.2
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