Analysis of capsid portal protein and terminase functional domains: Interaction sites required for DNA packaging in bacteriophage T4

Journal of Molecular Biology

Volumn 289, Issue 2, 1999, Pages 249-260

  Lin, Hsingchi ^a   Rao, Venigalla B ^a   Black, Lindsay W ^a  

^a UNIVERSITY OF MARYLAND   (United States)

Author keywords

DNA packaging; Portal protein; Terminase; Viral capsid; Virus assembly

Indexed keywords

CAPSID PROTEIN; POLYCLONAL ANTIBODY; PROTEIN SUBUNIT; VIRUS ENZYME;

AMINO ACID SEQUENCE; ARTICLE; BACTERIOPHAGE LAMBDA; BACTERIOPHAGE T3; BACTERIOPHAGE T4; BINDING AFFINITY; BIOCHEMISTRY; COLUMN CHROMATOGRAPHY; COMPARATIVE STUDY; COMPETITIVE INHIBITION; CONTROLLED STUDY; DNA PACKAGING; GENE LOCATION; GENE MUTATION; GENE SEQUENCE; HYDROPHOBICITY; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN DOMAIN; SUPPRESSOR GENE; VIRUS CAPSID; VIRUS RECOMBINANT;

EID: 0033523081     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.2781     Document Type: Article

References (40)

1. Black L. W. In vitro packaging of bacteriophage T4 DNA. Virology. 113:1981;336-344.
2. Black L. W. DNA packaging in dsDNA bacteriophages. Annu. Rev. Microbiol. 43:1989;267-292.
3. Black L. W. DNA packaging and cutting by phages terminases: control in phage T4 by a synaptic mechanism. Bioessays. 17:1995;1025-1030.
4. Casjens S., Hendrix R. Control mechanisms in dsDNA bacteriophage assembly. Calendar R. The Bacteriophages. 1988;15-91 Plenum Press, New York.
5. Catalano C. E., Tomka M. A. Role of gpFI protein in DNA packaging by bacteriophage lambda. Biochemistry. 34:1995;10036-10042.
6. Cox J. H., Strack H. B. Cold-sensitive mutants of bacteriophage λ Genetics. 67:1971;5-17.
7. Davidson A., Gold M. A novel in vitro DNA packaging system demonstrating a direct role for the bacteriophage λ F1 gene product. Virology. 161:1987;305-314.
8. Donate L. E., Carrascosa J. L. Characterization of a versatile in vitro DNA-packaging system based on hybrid λ/φ29 proheads. Virology. 182:1991;534-544.
9. Driedonks R. A., Caldentey J. Gene 20 product of bacteriophage T4. II. Its structural organization in prohead and bacteriophage. J. Mol. Biol. 166:1983;341-360.
10. Earnshaw W., Casjens S. DNA packaging by the double-stranded DNA bacteriophages. Cell. 21:1980;319-331.
11. Eppler K., Wyckoff E., Goates J., Parr R., Casjens S. Nucleotide sequence of the bacteriophage P22 genes required for DNA packaging. Virology. 183:1991;519-538.
12. Golz S., Kemper B. Association of Holliday-structure resolving endonuclease VII with gp20 from the packaging machine of phage T4. J. Mol. Biol. 285:1999;1131-1144.
13. Guo P., Peterson C., Anderson D. The DNA packaging protein gp16 of bacteriophage Phi29 is a prohead- and DNA-gp3-dependent ATPase. J. Mol. Biol. 197:1987;229-236.
14. Hong Y. R., Black L. W. An expression- packaging-processing vector which selects and maintains 7-kb DNA inserts in the blue T4 phage genome. Gene. 136:1993;193-198.
15. Hsiao C. L., Black L. W. DNA packaging and the pathway of bacteriophage T4 assembly. Proc. Natl Acad. Sci. USA. 74:1977;3652-3656.
16. Karam J. D., Trojanowska M., Baucom M. Interactions of T4-induced enzymes in vivo: inferences from intergenic suppression. Mathews C. K., Kutter E. M., Mosig G., Berget P. B. Bacteriophage T4. 1983;334-341 ASM Press, Washington.
17. Kuebler D., Rao V. B. Functional analysis of the DNA packaging/terminase protein gp17 from bacteriophage T4. J. Mol. Biol. 281:1998;803-814.
18. Kutter E., Stridham T., Guttman B., Kutter E., Batts D., Peterson S., Djavakhishvili T., Arisaka F., Mesyanzhinov V., Ruger W., Mosig G. Genomic map of bacteriophage T4. Karam J. D. Molecular Biology of Bacteriophage T4. 1994;491-519 ASM Press, Washington.
19. Lin H. The mechanism of the phage T4 terminase complex, gp16 and gp17, in DNA packaging. PhD thesis. 1996;University of Maryland at Baltimore.
20. Lin H., Simon M. N., Black L. W. Purification and characterization of the small subunit of phage T4 terminase, gp16, required for DNA packaging. J. Biol. Chem. 272:1997;3495-3501.
21. Michaud G., Zachary A., Rao V. B., Black L. W. Membrane-associated assembly of a phage T4 DNA entrance vertex structure studied with expression vectors. J. Mol. Biol. 209:1989;667-681.
22. Morita M., Tasaka M., Fujisawa H. DNA packaging ATPase of bacteriophage T3. Virology. 193:1993;748-752.
23. Morita M., Tasaka M., Fujisawa H. Analysis of functional domains of the packaging proteins of bacteriophage T3 by site-directed mutagenesis. J. Mol. Biol. 235:1994;248-250.
24. Morita M., Tasaka M., Fujisawa H. Analysis of the fine structure of the prohead binding domain of the packaging protein of bacteriophage T3 using a hexapeptide, an analog of a prohead binding site. Virology. 211:1995;516-524.
25. Mosig G., Ghosal D., Bock S. Interactions between the maturation protein gp17 and the single-stranded DNA binding protein gp32 initiate DNA packaging and compete with initiation of secondary DNA replication forks in phage T4. duBow M. S. Bacteriophage Assembly. 1981;139-150 Alan R. Liss Inc. New York.
26. Murialdo H. Bacteriophage lambda DNA maturation and packaging. Annu. Rev. Biochem. 60:1991;125-153.
27. Rao V. B., Black L. W. DNA packaging of bacteriophage T4 proheads invitro: evidence that prohead expansion is not coupled to DNA packaging. J. Mol. Biol. 185:1985;565-578.
28. Rao V. B., Black L. W. Cloning, overexpression and purification of the terminase protein gp16 and gp17 of bacteriophage T4: construction of a defined invitro system using purified terminase protein. J. Mol. Biol. 200:1988;475-488.
29. Ren Z. J., Black L. W. Phage T4 SOC and HOC display of biologically active, full-length proteins on the viral capsid. Gene. 215:1998;439-444.
30. Ren Z. J., Lewis G. K., Wingfield P. T., Locke E. G., Steven A. C., Black L. W. Phage display of intact domains at high copy number: a system based on SOC, the small outer capsid protein of bacteriophage T4. Protein Sci. 5:1996;1833-1843.
31. Ren Z. J., Baumann R., Black L. W. Cloning of linear DNAs in vivo by over expressed T4 DNA ligase: construction of a T4 phage hoc gene display vector. Gene. 195:1997;303-311.
32. Robey F. A., Fields R. L. Automated synthesis of N-bromoacetyl modified peptides for the preparation of synthetic peptide polymers, peptide-protein conjugates, and cyclic peptides. Anal. Biochem. 177:1989;373-377.
33. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
34. Valpuesta J. M., Carrascosa J. L. The structure and function of viral connectors. Quart. Rev. Biophys. 27:1994;107-155.
35. Volker T. A., Showe M. K. Induction of mutants in specific genes of bacteriophage T4 using cloned restriction fragments and marker rescue. Mol. Gen. Genet. 177:1980;447-452.
36. Wu C. H. H., Lin H., Black L. W. Bacteriophage T4 gene 17 amplification mutants: evidence for initiation by the T4 terminase subunit gp16. J. Mol. Biol. 247:1995;523-528.
37. Wunderli H., van den Broek J., Kellenberger E. Studies related to the head-maturation pathway of bacteriophages T4 and T2: I. morphology and kinetics of intracellular particles produced by mutants in the maturation genes. J. Supramol. Struct. 7:1977;135-161.
38. Xin W., Cai Z.-H., Feiss M. Function of IHF in λ DNA packaging II. Effects of mutations altering the IHF binding site and the intrinsic bend in cosB on λ development. J. Mol. Biol. 230:1993;505-515.
39. Yanagida M., Suzuki Y., Toda T. Molecular organization of the head of bacteriophage Teven: underlying design and principles. Adv. Biophys. 17:1984;97-146.
40. Yeo A., Feiss M. Specific interaction of terminase, the DNA packaging enzyme of bacteriophage λ, with the portal protein of the prohead. J. Mol. Biol. 245:1995;142-150.