A pair of related genes with antagonistic roles in mediating flowering signals

Science

Volumn 286, Issue 5446, 1999, Pages 1960-1962

  Kobayashi, Yasushi ^a   Kaya, Hidetaka ^a   Goto, Koji ^a   Iwabuchi, Masaki ^a,b   Araki, Takashi ^a  

^a KYOTO UNIVERSITY   (Japan)

^b Research Institute for Biological Science OKAYAMA   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA;

ARABIDOPSIS; ARTICLE; GENE EXPRESSION; GENE MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PHOTOPERIODICITY; PLANT; PLANT GROWTH; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARABIDOPSIS PROTEINS; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; MADS DOMAIN PROTEINS; MOLECULAR SEQUENCE DATA; PHENOTYPE; PHOTOPERIOD; PLANT COMPONENTS; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTORS; UP-REGULATION;

EID: 0033520985     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5446.1960     Document Type: Article

References (31)

1. Y. Y. Levy and C. Dean, Plant Cell 10, 1973 (1998); M. Koornneef, C. Alonso-Blanco, A. J. M. Peeters, W. Soppe, Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 345 (1998).
2. Y. Y. Levy and C. Dean, Plant Cell 10, 1973 (1998); M. Koornneef, C. Alonso-Blanco, A. J. M. Peeters, W. Soppe, Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 345 (1998).
3. L. Ruiz-García et al., Plant Cell 9, 1921 (1997).
4. A T-DNA insertion line (vTAAT26C51) with a 75.8-kb deletion in the FT-FAS1 region was identified. The bacterial artificial chromosome (BAC) clone F5I14 (GenBank accession number AC001229) was found to cover the deleted region. One candidate gene (FSI14.3) with similarity to TFL1 (4) was examined in six ft alleles, including three new ones (ft-4, ft-5, and ft-6 from ecotype Nossen) [this study and (16)], and a nucleotide substitution was found in all cases. The entire FSI14.3 was deleted in vTAAT26C51. On the basis of these results, we concluded that F5I14.3 is the FT gene. Sequences of cONA were deposited in GenBank (accession numbers AB027504 and AB027505).
5. D. Bradley, O. Ratcliffe, C. Vincent, R. Carpenter, E. Coen, Science 275, 80 (1997); S. Oshima, M. Murata, W. Sakamoto, Y. Ogura, F. Motoyoshi, Mol. Gen. Genet. 254, 166 (1997).
6. D. Bradley, O. Ratcliffe, C. Vincent, R. Carpenter, E. Coen, Science 275, 80 (1997); S. Oshima, M. Murata, W. Sakamoto, Y. Ogura, F. Motoyoshi, Mol. Gen. Genet. 254, 166 (1997).
7. Other members are: TWIN SISTER OF FT (TSF, GenBank accession number AB027506), ARABIDOPSIS THALIANA CENTRORADIALIS (ATC, GenBank accession number AB024715), BROTHER OF FT AND TFL1 (BFT = MTG10.5, GenBank accession number AB016880), and MOTHER OF FT AND TFL1 (MFT, GenBank accession number AF147721). The nomenclature was decided through agreement between D. Weigel's group and ours.
8. The putative orthologs are a Citrus unshiu expressed sequence tag clone for CiFT (GenBank accession number AB027456), and a rice BAC clone, nbxb0035E07r (GenBank accession number AQ289409), containing a part of OsFT.
9. The period of floral commitment was determined by expression of pAP1::GUS and pAP3::GUS reporter genes.
10. J. Putterill, F. Robson, K. Lee, R. Simon, C. Coupland, Cell 80, 847 (1995).
11. R. Simon, M. I. Igeño, C. Coupland, Nature 384, 59 (1996).
12. H. Guo, H. Yang, T. C. Mockler, C. Lin, Science 279, 1360 (1998).
13. R. Macknight et al., Cell 89, 737 (1997).
14. D. Weigel and O. Nilsson, Nature 377, 495 (1995).
15. M. A. Mandel and M. F. Yanofsky, Nature 377, 522 (1995).
16. For additional data, see Science Online (www. sciencemag.org/feature/data/1044707.shl).
17. M. I. Igeño and G. Coupland, personal communication.
18. M. Koornneef, C. J. Hanhart, J. H. van der Veen, Mol. Gen. Genet. 229, 57 (1991).
19. S. Shannon and D. R. Meeks-Wagner, Plant Cell 3, 877 (1991).
20. J. Alvarez, C. L. Guli, X.-H. Yu, D. R. Smyth, Plant J. 2, 103 (1992).
21. O. J. Ratcliffe et al., Development 125, 1609 (1998).
22. Y. Kobayashi and T. Araki, unpublished data.
23. S. J. Liljegren, C. Gustafson-Brown, A. Pinyopich, G. S. Ditta, M. F. Yanofsky, Plant Cell 11, 1007 (1999).
24. F. Schoentgen and J. Jollès, FEBS Lett. 369, 22 (1995);
25. M. J. Banfield, J. J. Barker, A. C. F. Perry, R. L. Brady, Structure 6, 1245 (1998);
26. L. Serre, B. Vallée, N. Bureaud, F. Schoentgen, C. Zelwer, Structure 6, 1255 (1998).
27. N. Tohdoh, S. Tojo, H. Agui, K. Ojika, Brain Res. Mol. Brain Res. 30, 381 (1995).
28. Single-letter abbreviations for amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
29. J. Kyozuka, personal communication.
30. 35S::CO:GR; co-2 plants were grown on MS medium (3% sucrose, 0.8% agar) under LD (16 hours light/8 hours dark) conditions. On day 14, 5 ml of 10 μM dex solution was applied to the medium (25 ml) to give a final concentration of 1.7 μM.
31. We particularly thank D. Weigel for exchange of unpublished results and materials. We thank G. Coupland and Plant Bioscience Limited, M. Koornneef, M. Omura, M. Yanofsky, and the NSF-supported Arabidopsis Biological Resource Center for materials, and G. Coupland, M. Koornneef, and J. Kyozuka for communicating unpublished results. Supported by grants from the Ministry of Education, Science, Culture and Sports of Japan, and an RFTF grant from the Japan Society for the Promotion of Science (JSPS). Y.K. is a JSPS Research Fellow.