Epitope mapping of LB509, a monoclonal antibody directed against human α-synuclein

Neuroscience Letters

Volumn 269, Issue 1, 1999, Pages 13-16

  Jakes, Ross ^a   Crowther, R Anthony ^a   Lee, Virginia M Y ^b   Trojanowski, John Q ^c   Iwatsubo, Takeshi ^c   Goedert, Michel ^a  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b UNIVERSITY OF PENNSYLVANIA   (United States)

^c UNIVERSITY OF TOKYO   (Japan)

Author keywords

Synuclein; Dementia with Lewy bodies; Epitope mapping; Multiple system atrophy; Parkinson's disease; Transgenic mice

Indexed keywords

ALPHA SYNUCLEIN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY LB 509; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL INCLUSION; DEMENTIA; EPITOPE MAPPING; HUMAN; IMMUNOBLOTTING; IMMUNOREACTIVITY; LEWY BODY; NONHUMAN; PARKINSON DISEASE; PRIORITY JOURNAL; RAT; SEQUENCE ANALYSIS; SHY DRAGER SYNDROME;

ALPHA-SYNUCLEIN; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; EPITOPE MAPPING; HUMANS; LEWY BODIES; MICE; NERVE TISSUE PROTEINS; PEPTIDE FRAGMENTS; RATS; SEQUENCE HOMOLOGY, AMINO ACID; SYNUCLEINS;

EID: 0033516587     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(99)00411-5     Document Type: Article

References (15)

1. Baba M., Nakajo S., Tu P.H., Tomita T., Nakaya K., Lee V.M.Y., Trojanowski J.Q., Iwatsubo T. Aggregation of α-synuclein in Lewy bodies of sporadic Parkinson's disease and dementia with Lewy bodies. Am. J. Pathol. 152:1998;879-884.
2. Crowther R.A., Jakes R., Spillantini M.G., Goedert M. Synthetic filaments assembled from C-terminally truncated α-synuclein. FEBS Lett. 436:1998;309-312.
3. George J.M., Jin H., Woods W.S., Clayton D.F. Characterization of a novel protein regulated during the critical period for song learning in the zebra finch. Neuron. 15:1995;361-372.
4. Hong L., Ko H.W., Gwag B.J., Joe E., Lee S., Kim Y.T., Suh Y.H. The cDNA cloning and ontogeny of mouse α-synuclein. NeuroReport. 9:1998;1239-1246.
5. Jakes R., Spillantini M.G., Goedert M. Identification of two distinct synucleins in human brain. FEBS Lett. 345:1994;27-32.
6. Krüger R., Kuhn W., Müller T., Woitalla D., Graeber M., Kösel S., Przuntek H., Epplen J.T., Schöls L., Riess O. Ala30Pro mutation in the gene encoding α-synuclein in Parkinson's disease. Nat. Genet. 18:1998;106-108.
7. Maroteaux L., Scheller R.H. The rat brain synucleins; family of proteins transiently associated with neuronal membrane. Mol. Brain Res. 11:1991;335-343.
8. Polymeropoulos M.H., Lavedan C., Leroy E., Ide S.E., Dehejia A., Dutra A., Pike B., Root H., Rubenstein J., Boyer R., Stenroos E.S., Chandrasekharappa S., Athanassiadou A., Papapetropoulos T., Johnson W.G., Lazzarini A.M., Duvoisin R.C., Di Iorio G., Golbe L.I., Nussbaum R.L. Mutation in the α-synuclein gene identified in families with Parkinson's disease. Science. 276:1997;2045-2047.
9. Spillantini M.G., Schmidt M.L., Lee V.M.Y., Trojanowski J.Q., Jakes R., Goedert M. α-Synuclein in Lewy bodies. Nature. 388:1997;839-840.
10. Spillantini M.G., Crowther R.A., Jakes R., Hasegawa M., Goedert M. α-Synuclein in filamentous inclusions of Lewy bodies from Parkinson's disease and dementia with Lewy bodies. Proc. Natl. Acad. Sci. USA. 95:1998;6469-6473.
11. Spillantini M.G., Crowther R.A., Jakes R., Cairns N.J., Lantos P.L., Goedert M. Filamentous α-synuclein inclusions link multiple system atrophy with Parkinson's disease and dementia with Lewy bodies. Neurosci. Lett. 251:1998;205-208.
12. Tu P.H., Galvin J.E., Baba M., Giasson B., Tomita T., Leight S., Nakajo S., Iwatsubo T., Trojanowski J.Q., Lee V.M.Y. Glial cytoplasmic inclusions in white matter oligodendrocytes of multiple system atrophy brains contain insoluble α-synuclein. Ann. Neurol. 44:1998;415-422.
13. Uéda K., Fukushima H., Masliah E., Xia Y., Iwai A., Yoshimoto M., Otero D.A.C., Kondo J., Ihara Y., Saitoh T. Molecular cloning of cDNA encoding an unrecognized component of amyloid in Alzheimer disease. Proc. Natl. Acad. Sci. USA. 90:1993;11282-11286.
14. Wakabayashi K., Yoshimoto M., Tsuji S., Takahashi H. α-Synuclein immunoreactivity in glial cytoplasmic inclusions in multiple system atrophy. Neurosci. Lett. 249:1998;180-182.
15. Wakabayashi K., Hayashi S., Kakita A., Yamada M., Toyoshima Y., Yoshimoto M., Takahashi H. Accumulation of α-synuclein/NACP is a cytopathological feature common to Lewy body disease and multiple system atrophy. Acta Neuropathol. 96:1998;445-452.